Essential fatty acid restriction inhibits vitamin D-dependent calcium absorption

Essential fatty acid (EFA) restriction has been found to inhibit the action of vitamin D on the active transport of calcium in the intestine. This inhibition suggests EFAs are involved in facilitating the active transport of calcium across the mucosal membrane.     

Comparison of three behavioral techniques in the modification of smoking behavior.

Compared 3 behavioral techniques-relaxation, satiation, and combined relaxation-satiation-to determine relative effectiveness in reducing cigarette smoking behavior in 53 volunteer smokers. Results indicate that all 3 approaches were effective initially, but that only the combined relaxation-satiation technique effectively maintained a significant reduction in cigarette consumption over a 3-mo follow-up period. (22 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Localization of the gene encoding the human heat shock cognate protein, HSP73, to chromosome 11

The heat shock cognate protein HSP73 (or HSC70) is a member of the HSP70 multigene family. This protein has several functions, including binding to nascent polypeptides to facilitate correct folding and the uncoating of clathrin-coated vesicles. Analysis of somatic cell hybrids by two-dimensional protein gel electrophoresis revealed the presence of a 73-kDa protein in two hybrids containing human chromosomes 5, 6, 9, and 11 in common. Using Western blot analysis, we demonstrate that this protein is a member of the HSP70 family and, by Southern blot analysis, that the HSP73 gene is located on human chromosome 11. Fluorescence in situ hybridization further localized HSP73 to the region 11q23.3-q25. This region is involved in a number of genetic rearrangements and is associated with several well-characterized tumours.     

Quality aspects of heated-air drying of soybeans

Samples of 300 g of soybean seed, variety Forrest, conditioned to 14, 16 and 18% moisture content (m.c.; w.b.), were dried for 4 hr in a small batch fluidized-bed rig with a test section of 120 mm diameter and air flow rate set at 0.03 kg/s. Air inlet temperatures of 40, 50, 55, 60, 65, 70 and 80°C were used to determine the effects on quality. Conditioned and unconditioned samples, together with samples dried in the bed for 4 hr at room temperature, served separately as controls. Samples of 25 g of the conditioned seed, sealed in test tubes, were heated for 4 hr in an air oven at 40, 45, 50, 55 and 60°C to study the effect of heating at a constant m.c.Seed germination and seedling vigour clearly indicated the onset of heat damage and showed that no single criterion was more sensitive than any other. Safe drying air temperatures of 65, 60 and 55°C were obtained for initial moisture contents of 14, 16 and 18% respectively. Heating soybeans up to 60°C at a fixed m.c. increased the susceptibility to heat damage. Oil vield, free fatty acid content and fatty acid composition were not affected by any of the heat treatments. The peroxide value, however, showed some increase at temperatures above 50°C. Fluidizing at room temperature did not affect germination.The colour of crude oil, after heat bleaching, for 50–80°C heat treatments was paler than the controls, while 40°C gave a very dark colour. Degumming of oils followed by refining removed all detectable phospholipids from all samples and produced oils paler in colour, when heat bleached, except for 80°C at 16 and 18% m.c. which produced oils slightly but noticeably darker.Soybeans were found to dry slowly compared to sunflower seed and rapeseed, and the logarithmic drying model was fitted to the various drying curves. Computer program DRIER was modified to be applicable to soybeans and some runs were carried out for a farm batch radial-flow drier. Results indicated the advantage of using a high inlet air temperature provided that seed quality is not affected.     

Culture conditions affect photoreactivating enzyme levels in human fibroblasts

Photoreactivation of pyrimidine dimers in mammalian cells occurs under our experimental conditions but has not been observed under conditions used by others. We have tested three possible differences in experimental procedures including dimer separation and analysis methods, illumination conditions and cell culture techniques. We show that out methods of dimer separation and analysis indeed measure cis-syn pyrimidine dimers and give results in quantitative agreement with the methods of others. We find that while light pre-illumination of fibroblasts from the xeroderma pigmentosum line XP12BE or of normal cells does not affect the cellular capacity for dimer photoreactivation. However, we show that cell culture conditions can affect photoreactivating enzyme levels and thus, cellular dimer photoreactivation capacity. Cells grown in Eagle's minimal essential medium (supplemented with 15% fetal bovine serum) contain very low levels of photoreactivating enzyme and cannot photoreactivate dimers in their DNA; however, companion cultures maintained in Dulbecco's modified Eagle's minimal medium do contain photoreactivating enzyme and can photoreactivate cellular dimers.     

Simultaneous pancreas-kidney transplant in two children with hemolytic-uremic syndrome

Simultaneous pancreas-kidney (SPK) transplantation has rarely been performed in the pediatric population. This report describes successful SPK transplantation in a 12-year-old girl and a 14-year-old boy with renal and pancreatic insufficiency secondary to postdiarrheal hemolytic-uremic syndrome. All reported cases of pediatric SPK transplantation are reviewed. SPK transplantation is a feasible option in selected pediatric patients with combined pancreatic and renal insufficiency.     

Te Papa Tongarewa — The Museum of New Zealand: Electronic data transfer and large public projects

The organizational system has succeeded in producing bids for the construction contracts tendered thus far almost precisely in line with the original estimates - an unusual claim on large scale public projects. CAD has been used as an information transfer mechanism and the conclusion to date is that despite numerous problems, the project would probably not have been possible without it.     

Conversational ethics in psychological dialogues: Discursive and collaborative considerations.

With the linguistic turn in the social sciences have come increased sensitivities to language use. In this paper, we examine such sensitivities as they relate to the conversational practices of psychologists seeking collaborative relationships with clients. In particular, we link ethical practice with developments in discourse theory and research, presenting arguments and evidence for enhanced forms of collaboration and client-centred practice. We conclude with considerations for what we consider "conversational ethics" in psychological practice. (PsycINFO Database Record (c) 2010 APA, all rights reserved)