Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesis

Cdc7 kinase is essential for initiation of DNA replication. Cdc7(-/-) mouse embryonic stem (ES) cells are non-viable but their growth can be rescued by an ectopically expressed transgene (Cdc7(-/-)tg). Here we report that, despite the normal growth capability of Cdc7(-/-)tg ES cells, the mice with the identical genetic background exhibit growth retardation. Concomi tantly, Cdc7(-/-)tg embryonic fibroblasts (MEFs) display delayed S phase entry and slow S phase progression. Furthermore, spermatogenesis of Cdc7(-/-)tg mice is disrupted prior to pachytene stage of meiotic prophase I. The impairment in spermatogenesis correlates with the extremely low level of Cdc7 protein in testes, and is rescued by introducing an additional allele of transgene, which results in increase of Cdc7 expression. The increased level of Cdc7 also recovers the growth of Cdc7(-/-)tg MEFs and mice, indicating that the developmental abnormalities of Cdc7(-/-)tg mice are due to insufficiency of Cdc7 protein. Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals.     

Cross-phase-modulation-induced nonlinear phase noise in WDM direct-detection DPSK systems

In direct-detection differential-phase-shift-keying (DPSK) systems, the signal-spontaneous emission beat noise introduces phase noise due to Kerr nonlinearity. In wavelength-division-multiplexed (WDM) systems, this nonlinear phase noise comes not only from the channel itself through self-phase modulation (SPM) but also from the other channels through cross-phase modulation (XPM). In this paper, we theoretically and experimentally investigate XPM-induced nonlinear phase noise. We calculate the performance degradation of DPSK systems caused by XPM-induced nonlinear phase noise in addition to SPM-induced phase noise and experimentally verify it using a 600-km nonzero-dispersion-shifted-fiber link.     

Test Technology Technical Council Newsletter

This newsletter provides information on past and upcoming events related to the IEEE Computer Society's Test Technology Technical Council and the test community.     

Insight into the chemical reaction of CH2OH with NO: Formation of N-hydroxy formamide as an isocyanic acid precursor

This study has explored the potential energy surface on the chemical reaction of CH₂OH with NO by using ab initio calculation. We have found the new reaction pathway producing N-hydroxy formamide, which can further decompose to generate isocyanic acid as a reducing agent of hydrocarbons selective catalytic reduction.     

Radiative and conductive transfer for a real gas in a cylindrical enclosure with gray walls

The purpose of this study is to examine the interaction of radiative and conductive transfer for a radiatively participating real gas stagnant in a cylindrical enclosure with gray diffuse walls. Consideration of reflecting boundaries represents an extension of previous black wall studies. Examination of radiative transfer was made by the zone method with gas radiative properties furnished by the weighted sum of gray gases model. Directed flux areas are expressed as the weighted sum of gray gas total exchange areas which are evaluated using the matrix formulation method from direct exchange areas. Axial and radial gas temperatures are examined along with wall heat flux or temperature for respective cases of either specified wall temperatures or heat fluxes. Emphasis is placed on examining results to show the effects of wall emittance and duct diameter. Results for heat generation within the gas are also presented.     

Strategy for olive mill wastewater treatment and reuse with a sewage plant in an arid region.

This study was conducted to evaluate the treatability of OMW (olive mill wastewater) with sewage and sewage sludge, which could supplement nutrients and microbes required for OMW treatment and reduce its possible toxicity. The amount of OMW added to an aeration tank was based on the loading difference between the designed and actual COD loads, while the amount added to anaerobic digestion for energy recovery was determined by CH4 production. The COD removal efficiencies were 70-85% for both systems. Compost of OMW with dried sewage sludge also showed a similar temperature profile without OMW addition. This strongly suggested that OMW can be treated at a sewage plant without pretreatment and the treated effluent can be reused in irrigation for an arid region.     

Possibility of sewage and combined sewer overflow reuse with biological aerated filters.

The secondary effluent from municipal plants in Korea generally represents higher BOD with lower SS. Therefore, more soluble forms of organics and NH4-N need to be removed to improve its effluent for reuse. In this study reuse possibility of secondary effluent and CSO (combined sewer overflow) using BAF (biological aerated filter) was evaluated. The tertiary application with 1.2 h EBCT, SS, BOD and COD showed stable concentrations less than 1.3, 1.3 and 6.2 mg/L, respectively. Nitrogen could be nitrified even at 7 degrees suggesting BAF can be used for a water reuse method as well as an effective add-on facility in cold regions. However, BAF was not stable with CSO application at increased flow rates suggesting CSO must be equalised prior to application. Disinfection was necessary even during normal weather conditions.     

A new resource allocation scheme based on a PSNR criterion forwireless video transmission to stationary receivers over Gaussianchannels

A new resource allocation scheme is proposed for video service over a Gaussian channel. For video quality rating, we introduce a suitable metric: the average peak signal-to-noise ratio (PSNR) or the average channel-induced PSNR degradation. Then, we model the end-to-end distortion considering the error propagation effect caused by motion compensation. On the basis of this model, we propose an efficient resource allocation scheme for real video service. Unlike conventional schemes, the new scheme satisfies the PSNR requirement of each video user by adjusting the bit-error rate level with the changes of image characteristics. Simulation results show that the proposed scheme utilizes the bandwidth more efficiently than conventional schemes     

Noise bandwidth suppression for low noise readout circuit

A new noise reduction circuit that suppresses noise bandwidth of the output stage is proposed for the readout circuit of an infrared detector operating at a high pixel rate. Using this circuit, it is found that the rms noise voltage of the output stage is effectively reduced from 87 to 52 μV at a pixel rate of 10 pixel/μs