准确计算摩擦系数

介绍一种非常准确的摩擦系数计算公式,它可以直接求解,而不用通常的试差法。     

分散剂在乳胶漆中的应用

综合实验结果和实际使用情况，讨论了涂料生产中聚磷酸盐与聚丙烯酸盐分散剂的应用情况，包括二者的配用以及不同涂料体系中分散剂的选择。     

Isolation and amino acid sequence of a serine proteinase inhibitor from common flax (Linum usitatissimum) seeds

LUTI (Linum usitatissimum trypsin inhibitor), a member of the potato inhibitor I family, has been isolated from seeds of flax by ethanol fractionation, ion exchange chromatography on CM-Sephadex C-25, affinity purification on immobilized methylchymotrypsin (alpha-chymotrypsin in which His57 has been converted to 3-methylhistidine) in the presence of 5M NaCl, and finally by reversed-phase HPLC. The 7655 Da inhibitor consists of a single polypeptide chain of 69 residues with one disulfide bridge. The molecule is acetylated at the N terminus. Its primary structure has been determined after limited proteolysis of the native molecule with trypsin at the reactive site, cleavage with cyanogen bromide or arginyl endopeptidase (Arg-gingipain), and alcoholytic deacetylation of the N-terminally blocked serine. The association constants (K(a)) of LUTI with bovine beta-trypsin and alpha-chymotrypsin are 3.58x10(10) M(-1) and 5.02x10(5) M(-1), respectively. High NaCl concentration (3M) increased the association constant of LUTI with alpha-chymotrypsin to 6.64x10(7) M(-1). To our knowledge, LUTI is the first serine-proteinase-type inhibitor isolated from a plant of the Linaceae family.     

High oleic acid oil suppresses lung tumorigenesis in mice through the modulation of extracellular signal-regulated kinase cascade

This study was undertaken to estimate the effect of dietary high oleic acid oil (OA) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis in mice. Diet containing 10% oil was fed to mice through experimental periods. On day 30 after NNK injection (100 mg/kg body weight, i.p.), the treatment increased the level of prostaglandin E₂ (PGE₂) as well as proliferating cell nuclear antigen, a marker of cell proliferation in a high linoleic acid oil (LA)-fed group but not in an OA-fed group. The NNK treatment also induced the activation of an extracellular signal-regulated kinase (Erk) cascade (Erk, Mek and Raf-1) in an LA-fed group. On the other hand, OA feeding abolished the NNK-induced activation of the Erk cascade. In conjugation with these events, OA feeding reduced lung tumor incidence and tumor multiplicity (percentage of mice with tumors) in mice compared with LA feeding at the 20th experimental week. These results suggest that OA suppresses lung tumorigenesis and that this suppression is correlated with the inhibition of PGE₂ production and inactivation of the Erk cascade.     

一种结构新颖的热交换器

<正> 最近,笔者研制了一种结构新颖的热交换器。这种热交换器可在很大程度上克服常规热交换设备所存在的两个不足之处,即换热表面的污染和密封垫圈的泄漏。新的换热器采用了新的结构和新的流体运动模式,相比现有的其他各种换热设备,它能向使用单位提供更多的便利。 在常规换热器使用过程中,被加热或冷     

Potential bile acid metabolites. 24. An efficient synthesis of carboxyl-linked glucosides and their chemical properties

A facile and efficient synthesis of the carboxyl-linked glucosides of bile acids is described. Direct esterification of unprotected bile acids with 2,3,4,6-tetra-O-benzyl-d-glucopyranose in pyridine in the presence of 2-chloro-1,3,5-trinitrobenzene as a coupling agent afforded a mixture of the α- and β-anomers (ca. 1∶3) of the 1-O-acyl-d-glucoside benzyl ethers of bile acids, which was separated effectively on a C₁₈ reversedphase chromatography column (isolated yields of α- and β-anomers are 4–9% and 12–19%, respectively). Subsequent hydrogenolysis of the α- and β-acyl glucoside benzyl ethers on a 10% Pd−C catalyst in acetic acid/methanol/EtOAc (1∶2∶2, by vol) at 35°C under atmospheric pressure gave the corresponding free esters in good yields (79–89%). Chemical specificities such as facile hydrolysis and transesterification of the acyl glucosides in various solvents were also discussed.     

Improvement of cyclodextrin glucanotransferase as an antistaling enzyme by error-prone PCR

In an effort to improve the properties of cyclodextrin glucanotransferase (CGTase) as an antistaling enzyme, error-prone PCR was used to introduce random mutations into a CGTase cloned from alkalophilic Bacillus sp. I-5 (CGTase I-5). A mutant CGTase[3-18] with the three mutations M234T, F259I and V591A was selected by agar plate assay. Sequence alignment of various CGTases indicated that M234 and F259 are located in the vicinity of the catalytic sites of the enzyme and V591 in the starch binding domain E. The cyclization activity of CGTase[3-18] was dramatically decreased by 10-fold, while the hydrolyzing activity was increased by up to 15-fold. These mutations near subsite +1 (M234T) and at subsite +2 (F259I) are likely to alter the enzyme activity in a concerted manner, promoting hydrolysis of substrate while retarding cyclization. The addition of CGTase[3-18] reduced the retrogradation rate of bread by as much as did the commercial antistaling enzyme Novamyl during 7-day storage at 4 degrees C. No cyclodextrin (CD) was detected in bread treated with CGTase[3-18], whereas 21 mg of CD per 10 g of bread was produced in bread treated with wild-type CGTase.     

现代生活的绘画——从马奈及其追随者的艺术看巴黎

本文是美国著名艺术理论家、批评家T.J.克拉克同名著作修订版的导论部分,探讨了印象派绘画与巴黎的关系.     

Nonlinear behavior of concrete beams with hybrid FRP and stainless steel reinforcements

The full-range behavior of partially bonded, together with partially prestressed concrete beams containing fiber reinforced polymer (FRP) tendons and stainless steel reinforcing bars was simulated using a simplified theoretical model. The model assumes that a section in the beam has a trilinear moment—curvature relationship characterized by three particular points, initial cracking of concrete, yielding of non-prestressed steel, and crushing of concrete or rupturing of prestressing tendons. Predictions from the model were compared with the limited available test data, and a reasonable agreement was obtained. A detailed parametric study of the behavior of the prestressed concrete beams with hybrid FRP and stainless steel reinforcements was conducted. It can be concluded that the deformability of the beam can be enhanced by increasing the ultimate compressive strain of concrete, unbonded length of tendon, percentage of compressive reinforcement and partial prestress ratio, and decreasing the effective prestress in tendons, and increasing in ultimate compressive strain of concrete is the most efficient one. The deformability of the beam is almost directly proportional to the concrete ultimate strain provided the failure mode is concrete crushing, even though the concrete ultimate strain has less influence on the load-carrying capacity. Foundation item: Project (50478502) supported by the National Natural Science Foundation of China