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排序方式: 共有287条查询结果,搜索用时 31 毫秒
281.
Yamawaki S Matsumoto T Ohnishi Y Kumada Y Shiomi N Katsuda T Lee EK Katoh S 《Journal of Bioscience and Bioengineering》2007,104(5):403-407
This study examined the effects of two methods of methanol feeding, DO-stat and methanol concentration control, in fed-batch and continuous cultures of Pichia pastoris on cell growth and single-chain variable fragment antibody (scFv) expression. By maintaining the methanol concentration at 3.9 g l(-1) in fed-batch culture, a scFv concentration of 198 mg l(-1) was obtained. In continuous culture using both methanol feeding methods, the scFv concentration in the fermentation broth increased with a decreasing dilution rate. A maximum scFv concentration of 810 mg l(-1) at a dilution rate of 0.0094 h(-1) was obtained by maintaining the methanol concentration at 3.9 g l(-1). Although the specific methanol consumption rate was the same for both methods, the specific productivity of scFv was higher in methanol concentration control from 0.0094 to 0.049 h(-1) than it was in DO-stat control. Therefore, continuous culture with methanol feeding by the concentration control method shows promise for the industrial scale production of recombinant proteins by Pichia pastoris. 相似文献
282.
283.
The mechanism by which a liquid may become arrested, forming a glass or gel, is a long-standing problem of materials science. In particular, long-lived (energetically) locally favoured structures (LFSs), the geometry of which may prevent the system relaxing to its equilibrium state, have long been thought to play a key role in dynamical arrest. Here, we propose a definition of LFSs which we identify with a novel topological method and directly measure with experiments on a colloidal liquid-gel transition. The population of LFSs is a strong function of (effective) temperature in the ergodic liquid phase, rising sharply approaching dynamical arrest, and indeed forms a percolating network that becomes the 'arms' of the gel. Owing to the LFSs, the gel is unable to reach equilibrium, crystal-gas coexistence. Our results provide direct experimental observation of a link between local structure and dynamical arrest, and open a new perspective on a wide range of metastable materials. 相似文献
284.
Utsunomiya T Yamane Y Watanabe M Sasaki K 《Journal of Bioscience and Bioengineering》2003,95(4):401-404
The effects of an external magnetic field on the production of porphyrin were investigated using Rhodobacter sphaeroides IF012203 under anaerobic-light conditions. Upon application of a 0.13-0.3-T magnetic field, the growth was slightly suppressed and porphyrin extracellular production was activated at both the N and S poles, particularly at the N pole up to about 5.3 times that in the control experiment (6.7 mg/1) (without magnetic filed application). The maximum production was 35.8 mg/1 at the N pole with a magnetic field of 0.3 T. At the same time, the 5-aminolevulinic acid dehydratase (ALAD) concentration was enhanced in the cells at the N and S poles, but particularly at the the N pole. This suggested the possibility that the magnetic field might activate ALAD gene expression in R. sphaeroides IF012203 and result in enhanced porphyrin production. 相似文献
285.
Takehiro Imura 《Electrical Engineering in Japan》2013,183(1):51-62
The demand for wireless power transfer via magnetic resonance coupling is increasing. Magnetic resonance coupling is a new technology that achieves power transfers across a large air gap by using transmitting and receiving antennas. However, repeater antennas can enable power transmission across an even larger distance. These repeater antennas without cross coupling can be expressed as a T‐type equivalent circuit. Equivalent circuits that include cross coupling and mutual inductance, which is related to the antenna position, have not been studied. In this paper, a novel way to represent a repeater antenna by an equivalent circuit and a way to determine the mutual inductance are proposed and veri?ed by performing an electromagnetic ?eld analysis and experiment. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 183(1): 51–62, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.22360 相似文献
286.
Keisuke Mine Takehiro Kawashiri Mizuki Inoue Daisuke Kobayashi Kohei Mori Shiori Hiromoto Hibiki Kudamatsu Mayako Uchida Nobuaki Egashira Satoru Koyanagi Shigehiro Ohdo Takao Shimazoe 《International journal of molecular sciences》2022,23(16)
(1) Background: Oxaliplatin is used as first-line chemotherapy not only for colorectal cancer but also for gastric and pancreatic cancers. However, it induces peripheral neuropathy with high frequency as an adverse event, and there is no effective preventive or therapeutic method. (2) Methods: The effects of omeprazole, a proton pump inhibitor (PPI), on oxaliplatin-induced peripheral neuropathy (OIPN) was investigated using an in vivo model and a real-world database. (3) Results: In a rat model, oxaliplatin (4 mg/kg, i.p., twice a week for 4 weeks) caused mechanical hypersensitivity accompanied by sciatic nerve axonal degeneration and myelin sheath disorder. Repeated injection of omeprazole (5–20 mg/kg, i.p., five times per week for 4 weeks) ameliorated these behavioral and pathological abnormalities. Moreover, omeprazole did not affect the tumor growth inhibition of oxaliplatin in tumor bearing mice. Furthermore, clinical database analysis of the Food and Drug Administration Adverse Event Reporting System (FAERS) suggests that the group using omeprazole has a lower reporting rate of peripheral neuropathy of oxaliplatin-treated patients than the group not using (3.06% vs. 6.48%, p < 0.001, reporting odds ratio 0.44, 95% confidence interval 0.32–0.61). (4) Conclusions: These results show the preventing effect of omeprazole on OIPN. 相似文献
287.