控制棒钴吸收体的活度分布

应用单组中子微扰理论,对辐照后控制棒钴吸收体内活度分布进行理论分析,得到活度分布计算的解析表达式。对高通量工程试验堆(HFETR)首次出堆的2根控制棒钴吸收体进行数值计算。利用钴靶活度水下测量装置进行这2根钴吸收体活度分布的实验测量。计算与实验测量结果相符。通过理论计算所得到的活度分布可作为辐照后~(60)Co同位素分装依据。本文得到的控制棒相对价值曲线理论计算值与实验测量结果符合良好。     

PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation

Methylation is one of the many post-translational modifications that modulate protein function. Although asymmetric NG,NG-dimethylation of arginine residues in glycine-arginine-rich domains of eucaryotic proteins, catalyzed by type I protein arginine N-methyltransferases (PRMT), has been known for some time, members of this enzyme class have only recently been cloned. The first example of this type of enzyme, designated PRMT1, cloned because of its ability to interact with the mammalian TIS21 immediate-early protein, was then shown to have protein arginine methyltransferase activity. We have now isolated rat and human cDNA orthologues that encode proteins with substantial sequence similarity to PRMT1. A recombinant glutathione S-transferase (GST) fusion product of this new rat protein, named PRMT3, asymmetrically dimethylates arginine residues present both in the designed substrate GST-GAR and in substrate proteins present in hypomethylated extracts of a yeast rmt1 mutant that lacks type I arginine methyltransferase activity; PRMT3 is thus a functional type I protein arginine N-methyltransferase. However, rat PRMT1 and PRMT3 glutathione S-transferase fusion proteins have distinct enzyme specificities for substrates present in both hypomethylated rmt1 yeast extract and hypomethylated RAT1 embryo cell extract. TIS21 protein modulates the enzymatic activity of recombinant GST-PRMT1 fusion protein but not the activity of GST-PRMT3. Western blot analysis of gel filtration fractions suggests that PRMT3 is present as a monomer in RAT1 cell extracts. In contrast, PRMT1 is present in an oligomeric complex. Immunofluorescence analysis localized PRMT1 predominantly to the nucleus of RAT1 cells. In contrast, PRMT3 is predominantly cytoplasmic.     

Granular multiple kernel learning for identifying RNA-binding protein residues via integrating sequence and structure information

Neural Computing and Applications - RNA-binding proteins play an important role in the biological process. However, the traditional experiment technology to predict RNA-binding residues is...     

单片机在木板自动剪切机中的应用

由于某厂生产各种规格木板的十多台设备专机,全靠人工手动操作的问题。这些设备与现在日益发展的工业自动化设备相比存在着很大的缺陷。本文利用AT89C51单片机进行控制,并在原设备工作台上加装一台三相异步电动机和一条木板送料传送带,使得工人不用在车间工作就可以控制整个生产过程。显著提高了企业生产效率,达到降低生产成本的效果。     

RFID技术在智能交通管理中应用

本文主要介绍RFDI技术定义及系统工作原理,并重点关注RFID技术在智能交通领域的实际应用方式。同时RFID技术也成功解决了车辆自动识别和信息采集问题,为智能交通管理和服务系统的建立提供了强大的信息支撑和保障。     

Thermal conductivity of a Kondo superconductor in the gapless state

The thermal conductivity coefficient for a Kondo superconductor in the gapless state is obtained within the framework of the Matsuura, Ichinose, and Nagaoka theory. By expanding the heat current correlation function appearing in the Kubo formula for the thermal conductivity in powers of the order parameter, analytical expressions for the thermal conductivity to second power in the order parameter are obtained. The thermal conductivities for bothT _KT _c 0 andT _KT _c 0 superconductors are obtained.     

电子和β射线两用外推电离室的研制

本文研制了一台测量电子束和β射线吸收剂量的外推电离室,其空腔室壁材料可拆换并分别与空气、组织和硅等效,收集电极名义直径为1.5、3、10和30mm,空气空腔可变范围为0.2～15mm。该外推电离室具有在空腔内部测温,自动改变窗口上方材料厚度及采用微型计算机控制等特点。适用于测10 MeV能量以下,剂量率为10~5～10~(-3)Gy/h的电子或β射线吸收剂量。整个外推电离室自动测剂量系统在测~(204)TLβ放射源表面剂量率的不确定度为2.3％。测1.5 MeV电子束在硅和哈密瓜深部剂量率时,不确定度分别为4.0％和2.8％。     

THE EFFECT OF FURNACE ATMOSPHERE DUE TO COMBUSTION UPON HYDROGEN CONTENT IN LIQUID STEEL

<正> 在炼钢过程中,炉气中水汽是钢液中含氢的主要来源。就平炉而言,除了空气湿度和炼钢原材料带入炉中的水分以外,还有煤气燃烧生成的水汽。根据过去的研究结果,由于空气湿度直接或间接引起的水汽分压,一般波动于0.01—0.08个大气压之间,而煤气燃烧废气中的水汽分压则经常在0.1个大气压以上,有时甚至可以超过0.2     

From Natural Methylation to Versatile Alkylations Using Halide Methyltransferases

Halide methyltransferases (HMTs) enable the enzymatic synthesis of S-adenosyl-l -methionine (SAM) from S-adenosyl-l -homocysteine (SAH) and methyl iodide. Characterisation of a range of naturally occurring HMTs and subsequent protein engineering led to HMT variants capable of synthesising ethyl, propyl, and allyl analogues of SAM. Notably, HMTs do not depend on chemical synthesis of methionine analogues, as required by methionine adenosyltransferases (MATs). However, at the moment MATs have a much broader substrate scope than the HMTs. Herein we provide an overview of the discovery and engineering of promiscuous HMTs and how these strategies will pave the way towards a toolbox of HMT variants for versatile chemo- and regioselective biocatalytic alkylations.