Cloning of the gene encoding the Actinobacillus actinomycetemcomitans serotype b OmpA-like outer membrane protein

The gene encoding an outer membrane protein A (OmpA)-like, heat-modifiable Omp of Actinobacillus actinomycetemcomitans ATCC 43718 (strain Y4, serotype b) was cloned by a PCR cloning procedure. DNA sequence analysis revealed that the gene encodes a protein of 346 amino acid residues with a molecular mass of 36.9 kDa. The protein expressed by the cloned gene reacted with a monoclonal antibody to the previously described 29-kDa Omp (Omp29) of strain Y4. This monoclonal antibody reacted specifically with Omp29 of A. actinomycetemcomitans (serotype b), but not with any Omp of Escherichia coli, including OmpA. This protein exhibited characteristic heat modifiability on sodium dodecyl sulfate-polyacrylamide gels, showing an apparent molecular mass of 29 kDa when unheated and a mass of 34 kDa when heated. The N-terminal amino acid sequence of the protein expressed in E. coli perfectly matched those deduced from the purified Omp29 of strain Y4. The deduced amino acid sequence of the gene coding for Omp29 from serotype b matched completely (except for valine at position 321) that of a recently reported omp34 gene described for A. actinomycetemcomitans serotype c (NCTC 9710). Because of the conserved nature of the gene within these serotypes, we designated the gene described herein from serotype b as omp34.     

Optimal erasure protection strategy for scalably compressed data with tree-structured dependencies.

This paper is concerned with the transmission of scalably compressed data sources over lossy channels. Specifically, this paper is concerned with packet networks or, more generally, erasure channels. Previous work has generally assumed that the source elements form linear dependencies. The contribution of this paper is an unequal erasure protection algorithm which is able to take advantage of scalable data with more general dependency structures. In particular, the proposed scheme is adapted to data with tree-structured dependencies. The source elements are allocated to clusters of packets according to their dependency structure, subject to constraints on packet size and channel code-word length. Given a packet cluster arrangement, source elements are assigned optimal channel codes subject to a constraint on the total transmission length. Experimental results confirm the benefit associated with exploiting the actual dependency structure of the data.     

Quantum cascade lasers: ultrahigh-speed operation, optical wirelesscommunication, narrow linewidth, and far-infrared emission

Following an introduction to the history of the invention of the quantum cascade (QC) laser and of the band-structure engineering advances that have led to laser action over most of the mid-infrared (IR) and part of the far-IR spectrum, the paper provides a comprehensive review of recent developments that will likely enable important advances in areas such as optical communications, ultrahigh resolution spectroscopy and applications to ultrahigh sensitivity gas-sensing systems. We discuss the experimental observation of the remarkably different frequency response of QC lasers compared to diode lasers, i.e., the absence of relaxation oscillations, their high-speed digital modulation, and results on mid-IR optical wireless communication links, which demonstrate the possibility of reliably transmitting complex multimedia data streams. Ultrashort pulse generation by gain switching and active and passive modelocking is subsequently discussed. Recent data on the linewidth of free-running QC lasers (~150 kHz) and their frequency stabilization down to 10 kHz are presented. Experiments on the relative frequency stability (~5 Hz) of two QC lasers locked to optical cavities are discussed. Finally, developments in metallic waveguides with surface plasmon modes, which have enabled extension of the operating wavelength to the far IR are reported     

Concurrency techniques for arithmetic coding in JPEG2000

JPEG2000 is a recently standardized image compression algorithm. The heart of this algorithm is the coding scheme known as embedded block coding with optimal truncation (EBCOT). This contributes the majority of processing time to the compression algorithm. The EBCOT scheme consists of a bit-plane coder coupled to a MQ arithmetic coder. Recent bit-plane coder architectures are capable of producing symbols at a higher rate than the existing MQ arithmetic coders can absorb. Thus, there is a requirement for a high throughput MQ arithmetic coder. We examine the existing MQ arithmetic coder architectures and develop novel techniques capable of absorbing the high symbol rate from high performance bit-plane coders, as well as providing flexible design choices.     

Modeling the behavior of novice young drivers during the first year after licensure

Novice young drivers suffer from increased crash risk that translates into over-representation in road injuries. In order to effectively confront this problem, a better understanding of the driving behavior of novice young drivers and of its determinants is needed. This study analyzes the behavior of novice young drivers within a Graduated Driver Licensing (GDL) program. Data on driving behavior of 62 novice drivers and their parents, who voluntarily participated in this experiment, were collected using in-vehicle data recorders that calculate compound risk indices as measures of the risk taking behavior of drivers. Data were used to estimate a negative binomial model to identify major determinants that affect the driving behavior of young drivers during the first year after licensure. Estimation results suggest that the risk taking behavior of young drivers is influenced by gender, sensation seeking tendency, driving behavior of their parents, amount of supervised driving and level of parental monitoring.     

Heat exchange during evaporation from a falling water film

Works devoted to the experimental and theoretical study of the heat-exchange coefficient during the evaporation of water in a falling film are briefly surveyed. The results of an estimate of the effect on of the heat flux, density of flow, etc.,are described.Translated from Inzhenerno-Fizicheskii Zhurnal, Vol. 21, No. 6, pp. 1040–1043, December, 1971.     

A flexible structure for fully scalable motion-compensated 3-D DWT with emphasis on the impact of spatial scalability.

We investigate the implications of the conventional "t+2-D" motion-compensated (MC) three-dimensional (3-D) discrete wavelet/subband transform structure for spatial scalability and propose a novel flexible structure for fully scalable video compression. In this structure, any number of levels of "pretemporal" spatial wavelet decomposition are performed on the original full resolution frames, followed by MC temporal decomposition of the subbands within each spatial resolution level. Further levels of "posttemporal" spatial decomposition may be performed on the spatiotemporal subbands to provide additional levels of spatial scalability and energy compaction. This structure allows us to trade energy compaction against the potential for artifacts at reduced spatial resolutions. More importantly, the structure permits extensive study of the interaction between spatial aliasing, scalability and energy compaction. We show that where the motion model fails, the "t+2-D" structure inevitably produces misaligned spatial aliasing artifacts in reduced resolution sequences. These artifacts can be removed by using pretemporal spatial decomposition. On the other hand, we also show that the "t+2-D" structure necessarily maximizes compression efficiency. We propose different schemes to minimize the loss of compression efficiency associated with pretemporal spatial decomposition.