Ceramics possess osteoconductive properties but exhibit no intrinsic osteoinductive capacity. Consequently, they are unable to induce new bone formation in extra osseous sites. In order to develop bone substitutes with osteogenic properties, one promising approach consists of creating hybrid materials by associating in vitro biomaterials with osteoprogenitor cells. With this aim, we have developed a novel strategy of biomimetic modification to enhance osseointegration of hydroxyapatite (HA) implants. RGD-containing peptides displaying different conformations (linear GRGDSPC and cyclo-DfKRG) were grafted onto HA surface by means of a three-step reaction procedure: silanisation with APTES, cross-linking with N-succinimidyl-3-maleimidopropionate and finally immobilisation of peptides thanks to thiol bonding. Whole process was performed in anhydrous conditions to ensure the reproducibility of the chemical functionalisation. The three-step reaction procedure was characterised by high resolution X-ray photoelectron spectroscopy. Efficiency of this biomimetic modification was finally demonstrated by measuring the adhesion of osteoprogenitor cells isolated from HBMSC onto HA surface. 相似文献
Screen-printed n+–p–p+ solar cells were fabricated on Cz single crystalline Si material, with a 45 Ω/sq emitter and PECVD SiNx antireflective coating with a thickness of 700 Å, using different Ag pastes and commercial leaded reference paste (CN33-462, Ferro Corp.). Ag and Al contacts were co-fired using a mass-production line equipped with mesh belt conveyer furnace systems (Centrotherm thermal solution GmbH & Co. KG). The average results for single crystalline Si solar cells (156 cm2) are: Isc=5.043 A, Voc=0.621 V, Rs=0.0087 Ω, Rsh=15.3 Ω, FF=0.773, and Eff=16.45%. Rsh and fill factor values of fabricated cells were slightly higher when compared with the commercial leaded Ag paste, although cells were fabricated by metallizing the lead-free silver pastes. For the lead-free Ag paste used in this study, the line pattern continuity is retained with improved edge definition in sharp contrast to that of reference Ag paste. Average value of Rs was also equivalent approximately to that of the leaded Ag paste. 相似文献
Although land managers and policy-makers generally have a good experience of what result can be expected from their decisions,
they are often faced with difficulty when trying to communicate the visual impact of a management option to stakeholders,
particularly when the landscape exhibits a high cultural value. Three-dimensional visualization of the landscape is often
used for communicating with the stakeholders. A challenge in participatory methods for integrated assessment and policy planning
is to view future changes in land use, according to scenarios. A 3-D landscape visualization component, SLE (“Seamless Landscape
Explorer”), has been developed, which is launched after a scenario simulation to allow for exploration of landscape changes.
Pressures causing such changes are translated into changes in the spatial configuration of the landscape. The different types
of land-use are visualized thanks to a library of detailed textures, and vegetation can be added. This has been applied to
a study of four scenarios in the French Mediterranean region, which were set up as part of a participatory process for discussing
the planning of the regional peri-urban and agricultural policy, in an area dominated by the typical culturally sensitive
Mediterranean matorral, (“garrigue” shrubland) surrounding the Pic Saint-Loup mountain. Examples of visualization are shown
and discussed here. 相似文献
Non‐digestible carbohydrate fraction (NDCF) consists of a range of bioactive compounds that escape digestion in the small intestine. NDCF is mainly composed of dietary fibre (DF) and non‐digestible oligosaccharides (NDO). The objective of this work was to analyse directly and compare the NDCF in two Brazilian cultivars of soybean seed with commercial flour. Brazil is the second major soybean producer in the world. The seed cultivars showed on average a NDCF value of 32.80 g per 100 g dry weight (dw). Regarding DF, insoluble DF (IDF) was the main fraction amounting on average to 98%. Uronic acids were significantly higher than neutral sugar (NS) in IDF and soluble DF (SDF) fractions. There was a considerable amount of Klason lignin – on average 84.14% of DF. Regarding NS profile, mannose was the most important sugar in SDF, whereas galactose and arabinose were predominant in IDF. Stachyose was the main oligosaccharide in NDO. DF and NDO were in the same proportion (94.2:5.8) in tested cultivars. No differences in swelling, water retention, or oil retention capacities between seed cultivars were detected. This study on non‐digestible carbohydrate components in soybean seed gives a different approach to the current nutritional knowledge on protein and oil constituents. 相似文献
Using a longitudinal field design, this study tested the dynamics of an integrative model of social identity theory (SIT) and relative deprivation theory (RDT) with regard to relations between perceived sociostructural characteristics, perceived in-group identification, perceived fraternal deprivation (i.e., resentment) and identity-management-strategy preference. Trait-state analyses revealed that the dynamic relationship among constructs in the model can best be explained in terms of trait-dependent variation rather than sequentially ordered processing. The trait components of the variables replicate previous findings concerning SIT and RDT. However, stable functional relations between variables and their traitlike character contradict the notion that their underlying processes are linear and sequential. Rather, variables and their relations can be regarded as the product of parallel processes. The authors discuss the results as a challenge to core assumptions of SIT. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
The purpose of this study was to verify the validity of a French version of the Positive and Negative Perfectionism Scale developed by L. A. Terry-Short (1995). This instrument measures the type as well as the level of perfectionism in adolescents. The instrument was first administered to 734 7th, 9th, and 11th grade students. Four weeks later, 596 Ss were invited to answer the questionnaire once more. The factorial structure, temporal stability and internal consistency of each subscales of the translated version of the instrument correspond to those of the original version. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
Objective: The aim of this study was to evaluate a formulation made of poly(lactide-co-glycolide) (PLGA) nanoparticles containing azelaic acid for potential acne treatment.
Methods: Azelaic acid-loaded PLGA nanoparticles were prepared by spontaneous emulsification processes using poloxamer 188 as stabilizer. Several manufacturing parameters such as stirring rate, concentration of stabilizer and different recovery methods were investigated. Nanoparticles were evaluated in terms of size, zeta potential, encapsulation efficiency, release kinetics and permeation kinetics in vitro. Furthermore, in vitro toxicological studies were performed in Saccharomyces cerevisiae model.
Results: The results showed that by adjusting some formulation conditions it was possible to obtain nanoparticles with high loading and a controlled drug release. Freeze-dried recovery altered the nanoparticles structure by enhancing porous structures and mannitol was required to control the mean particle size. The centrifugation recovery was found to be the best approach to nanoparticles recovery. Similar toxicity profiles were observed for both drug-free and azelaic acid-loaded nanoparticles, with concentration-dependent decreases in cell viability.
Conclusion: These results indicate a potential formulation for controlled release delivery of azelaic acid to the follicular unit. 相似文献
Mobilization and deposition of iron nano and sub-micrometer particles (INSMP) in a porous medium were investigated using a water-saturated glass micromodel. The deposition and detachment of INSMP in the micromodel were visualized by taking serial images and experimentally verified by analysis of breakthrough curves. This first visualization study of INSMP fate showed that there were dense aggregations at the pores as the concentration of INSMP increased. The presence of dissolved humic substances (>1 ppm) significantly reduced deposition of suspended particles and enhanced detachment of the deposited particles. The mobility of INSMP in the presence of Pahokee peat fulvic acid standard II (PPFA) was higher than for Pahokee peat humic acid standard I (PPHA) due to the presence of more aromatic groups and the molecular weight in PPFA. Interfacial energy estimation based on the DLVO theory revealed that the adsorption of humic substances onto the INSMP increased the energy barrier and reduced the depth of secondary minimum between particles. The “affinity transition” in the initial deposition of INSMP within the micromodel was observed in the presence of Pahokee peat humic substances. 相似文献