Function of Graphene Oxide as the “Nanoquencher” for Hg2+ Detection Using an Exonuclease I-Assisted Biosensor

Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg²⁺ detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg²⁺–T when Hg²⁺ is present, and no fluorescence is observed. It is digested by exonuclease I without Hg²⁺, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg²⁺ concentration in the range of 0–250 nM (R² = 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg²⁺ detection, obtaining a recovery rate from 99.6% to 101.1%.     

实施汽轮机单阀改顺序阀运行效果分析

岱海发电厂Ⅰ期2台600MW汽轮机为上海汽轮机有限公司引进美国西屋技术生产的汽轮机,在单阀切顺序阀运行后存在瓦温高、轴振大、上下缸温差大等问题。针对这些问题,进行了单阀切换为顺序阀试验,对存在的问题采取了改进措施,有效地提高了机组效率。     

Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy

Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20–30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3′-untranslated regions (3′ UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation.     

负载氯喹的骨靶向纳米颗粒打破肿瘤细胞增殖与骨吸收之间的恶性循环

肿瘤细胞增殖与骨吸收之间的恶性循环加剧了骨肿瘤的进展和转移风险.为此,我们设计并制备了聚乙二醇-阿仑膦酸钠修饰的聚多巴胺(PPA)纳米粒子,并在其表面负载自噬抑制剂氯喹(CQ),期望利用该治疗载体(PPA/CQ)打破肿瘤细胞增殖与骨吸收之间的恶性循环,从而有效地治疗骨肿瘤.实验证明,PPA/CQ可以有效地富集到骨组织,...     

基于PIC16F946的CO气体检测仪

介绍了一种便携式CO气体检测仪的开发,论述了仪器的硬件原理图、一氧化碳气体传感器的参数模型分析以及软件的程序流程图,该仪器可实现检测空气中CO的含量,并可自动进行声、光以及震动报警,可通过按键设定二级报警值,并且可以对仪器进行标定,仪器以美国microchip公司的微控制器PIC16F946作为核心控制单元,分辨率为lppm;误差为士3%,采用3V的锂电池可以连续工作9000h以上,可广泛用于工业生产、环保、汽车、家庭等一氧化碳泄漏和不完全燃烧的检测报警.     

基于Moodle和PhoneGap的移动学习客户端的设计与应用研究

本文将结合用户移动学习现状及偏好,确定移动学习客户端的系统需求,进而基于Moodle和PhoneGap平台,实现对移动学习客户端的设计,并将移动学习客户端应用到实际教学过程中,确定应用成效。     

超长结构地下室外墙混凝土裂缝的控制

对超长结构地下室外墙板混凝土裂缝的控制,是施工中的一大难题。经实验研究,从"抗"、"放"二方面入手,即采用抗裂筋、混凝土掺外加剂以及加强保温、保湿养护等技术工艺后,使超长结构地下室外墙板混凝土裂缝问题得到了有效的控制。     

国家投资体制改革形势下的工程项目管理模式探索

上海市高级人民法院工程项目管理工作是运用建设、经济、技术、法律方面知识和经验实施管理的一项成功的工程咨询业务实例,同时也对国家投资体制改革形势下的工程咨询和项目管理模式进行了有益的探索。