全文获取类型
收费全文 | 839篇 |
免费 | 32篇 |
国内免费 | 2篇 |
专业分类
电工技术 | 39篇 |
综合类 | 2篇 |
化学工业 | 244篇 |
金属工艺 | 21篇 |
机械仪表 | 8篇 |
建筑科学 | 6篇 |
能源动力 | 24篇 |
轻工业 | 45篇 |
无线电 | 70篇 |
一般工业技术 | 124篇 |
冶金工业 | 218篇 |
原子能技术 | 25篇 |
自动化技术 | 47篇 |
出版年
2023年 | 3篇 |
2022年 | 5篇 |
2021年 | 19篇 |
2020年 | 15篇 |
2019年 | 15篇 |
2018年 | 18篇 |
2017年 | 8篇 |
2016年 | 19篇 |
2015年 | 5篇 |
2014年 | 26篇 |
2013年 | 41篇 |
2012年 | 36篇 |
2011年 | 26篇 |
2010年 | 32篇 |
2009年 | 39篇 |
2008年 | 28篇 |
2007年 | 19篇 |
2006年 | 25篇 |
2005年 | 26篇 |
2004年 | 23篇 |
2003年 | 21篇 |
2002年 | 22篇 |
2001年 | 14篇 |
2000年 | 17篇 |
1999年 | 25篇 |
1998年 | 73篇 |
1997年 | 46篇 |
1996年 | 36篇 |
1995年 | 30篇 |
1994年 | 22篇 |
1993年 | 24篇 |
1991年 | 10篇 |
1990年 | 6篇 |
1989年 | 7篇 |
1988年 | 9篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1984年 | 8篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1980年 | 3篇 |
1979年 | 6篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
排序方式: 共有873条查询结果,搜索用时 0 毫秒
871.
Arisa Kawamukai Ayana Iwano Momoka Shibata Yuko Kishi Akira Matsuura 《Yeast (Chichester, England)》2023,40(2):59-67
Changes in extracellular pH affect the homeostasis and survival of unicellular organisms. Supplementation of culture media with amino acids can extend the lifespan of budding yeast, Saccharomyces cerevisiae, by alleviating the decrease in pH. However, the optimal amino acids to use to achieve this end, and the underlying mechanisms involved, remain unclear. Here, we describe the specific role of serine metabolism in the regulation of pH in a medium. The addition of serine to synthetic minimal medium suppressed acidification, and at higher doses increased the pH. CHA1, which encodes a catabolic serine hydratase that degrades serine into ammonium and pyruvate, is essential for serine-mediated alleviation of acidification. Moreover, serine metabolism supports extra growth after glucose depletion. Therefore, medium supplementation with serine can play a prominent role in the batch culture of budding yeast, controlling extracellular pH through catabolism into ammonium and acting as an energy source after glucose exhaustion. 相似文献
872.
Dr. Hiroshi Inaba Minamo Sakaguchi Soei Watari Dr. Shigesaburo Ogawa Dr. Arif Md. Rashedul Kabir Prof. Dr. Akira Kakugo Prof. Dr. Kazuki Sada Prof. Dr. Kazunori Matsuura 《Chembiochem : a European journal of chemical biology》2023,24(8):e202200782
Spatiotemporal modulation of microtubules by light has become an important aspect of the biological and nanotechnological applications of microtubules. We previously developed a Tau-derived peptide as a binding unit to the inside of microtubules. Here, we conjugated the Tau-derived peptide to spiropyran, which is reversibly converted to merocyanine by light, as a reversible photocontrol system to stabilize microtubules. Among the synthesized peptides with spiropyran/merocyanine at different positions, several peptides were bound to the inside of microtubules and stabilized the structures of microtubules. The peptide with spiropyran at the N-terminus induced polymerization and stabilization of microtubules, whereas the same peptide with the merocyanine form did not exert these effects. Reversible formation of microtubules/tubulin aggregates was achieved using the peptide with spiropyran conjugated at the N-terminus and irradiation with UV and visible light. Spiropyran-conjugated Tau-derived peptides would be useful for spatiotemporal modulation of microtubule stability through reversible photocontrol of binding. 相似文献
873.
Yingbing Liang Hiroto Furukawa Dr. Kentarou Sakamoto Dr. Hiroshi Inaba Prof. Dr. Kazunori Matsuura 《Chembiochem : a European journal of chemical biology》2022,23(15):e202200220
Ribonuclease S (RNase S) is an enzyme that exhibits anticancer activity by degrading RNAs within cancer cells; however, the cellular uptake efficiency is low due to its small molecular size. Here we generated RNase S-decorated artificial viral capsids with a size of 70–170 nm by self-assembly of the β-annulus-S-peptide followed by reconstitution with S-protein at neutral pH. The RNase S-decorated artificial viral capsids are efficiently taken up by HepG2 cells and exhibit higher RNA degradation activity in cells compared with RNase S alone. Cell viability assays revealed that RNase S-decorated capsids have high anticancer activity comparable to that of standard anticancer drugs. 相似文献