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71.
Vanessa Harumi Kiyan Flávia Pires Rodrigues Ricardo Elgul Samad Denise Maria Zezell Marco Antonio Bottino Nelson Batista De Lima Cintia Helena Coury Saraceni 《Ceramics International》2021,47(4):4455-4465
Femtosecond pulses from a Ti:Sapphire laser were used to irradiate specimens of yttria-stabilised (35% mol) tetragonal zirconia (Y-TZP) with the purpose of studying the effects of the irradiations on their surface properties and morphology after ageing. Zirconia disks were divided into eight groups (n = 32) according to their surface treatment and subsequent ageing: Control: no treatment; sandblasting: Al2O3 sandblasting 50 μm; and ultrashort laser pulses irradiation with 25 μJ pulses, considering two different scanning steps based on the width between two grooves. These groups were duplicated and submitted to ageing. The surfaces were analysed using scanning electron microscopy (SEM), and X-ray diffraction. A finite element analysis, a biaxial flexure test, as well as fractographic and Weibull analyses, were performed. The strengths of the disks were statistically different for the treatment factor, and the principal stresses seemed to be concentrated at the centre of the specimens, as predicted by the computer simulations. Ageing decreased the strengths for all groups and increased the Weibull modulus for the laser group with the 40 μm-width between two grooves. The sandblasting group presented the highest monoclinic phase peak. Although the most significant strength was found within the sandblasting group, the phase transformation was favourable to the laser groups. The Weibull modulus was higher for the laser group with the 60 μm-width between two grooves, confirming the highest homogeneity of its failure distribution. Regardless of the surface treatment, strength was decreased with ageing in all groups. The femtosecond Ti:Sa ultra-short pulse laser irradiation can be suggested as an alternative to the gold standard sandblasting in long-term Y-TZP zirconia rehabilitations, such as crowns and veneers. 相似文献
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Morena Petrini Tania Vanessa Pierfelice Emira DAmico Natalia Di Pietro Assunta Pandolfi Camillo DArcangelo Francesco De Angelis Domitilla Mandatori Valeria Schiavone Adriano Piattelli Giovanna Iezzi 《International journal of molecular sciences》2021,22(18)
Current research on dental implants has mainly focused on the influence of surface roughness on the rate of osseointegration, while studies on the development of surfaces to also improve the interaction of peri-implant soft tissues are lacking. To this end, the first purpose of this study was to evaluate the response of human gingival fibroblasts (hGDFs) to titanium implant discs (Implacil De Bortoli, Brazil) having different micro and nano-topography: machined (Ti-M) versus sandblasted/double-etched (Ti-S). The secondary aim was to investigate the effect of the macrogeometry of the discs on cells: linear-like (Ti-L) versus wave-like (Ti-W) surfaces. The atomic force microscopy (AFM) and scanning electron microscopy (SEM) analysis showed that the Ti-S surfaces were characterized by a significantly higher micro and nano roughness and showed the 3D macrotopography of Ti-L and Ti-W surfaces. For in vitro analyses, the hGDFs were seeded into titanium discs and analyzed at 1, 3, and 5 days for adhesion and morphology (SEM) viability and proliferation (Cck-8 and MTT assays). The results showed that all tested surfaces were not cytotoxic for the hGDFs, rather the nano-micro and macro topography favored their proliferation in a time-dependent manner. Especially, at 3 and 5 days, the number of cells on Ti-L was higher than on other surfaces, including Ti-W surfaces. In conclusion, although further studies are needed, our in vitro data proved that the use of implant discs with Ti-S surfaces promotes the adhesion and proliferation of gingival fibroblasts, suggesting their use for in vivo applications. 相似文献
75.
Taisa Venäläinen Ursula Schwab Jyrki Ågren Vanessa de Mello Virpi Lindi Aino-Maija Eloranta Sanna Kiiskinen David Laaksonen Timo A. Lakka 《Lipids》2014,49(5):467-479
Plasma fatty acid (FA) composition is known to be an indicator of dietary fat quality, but the associations of other dietary factors with plasma FA composition remain unknown in children. We investigated the cross-sectional associations of food consumption with the proportions of FA and estimated desaturase activities in plasma cholesteryl esters (CE) and phospholipids (PL) among children. The subjects were a population sample of 423 children aged 6–8 years examined at baseline of The Physical Activity and Nutrition in Children (PANIC) Study. We assessed food consumption by food records and plasma FA composition by gas chromatography. We used linear regression models adjusted for age, sex, physical activity and total energy intake to analyze the associations. A higher consumption of vegetable oil-based margarine (fat 60–80 %) was associated with a higher proportion of linoleic and α-linolenic acids in plasma CE and PL. A higher consumption of high-fiber grain products was related to a lower proportion of oleic acid in CE and PL. The consumption of candy was directly associated with the proportion of palmitoleic and oleic acid in plasma CE. The consumption of vegetable oil-based margarine was inversely associated with estimated stearoyl-CoA-desaturase activity in plasma CE and PL and the consumption of candy was directly related to it in plasma CE. The results of our study suggest that plasma FA composition is not only a biomarker for dietary fat quality but also reflects the consumption of high-fiber grain products and foods high in sugar among children. 相似文献
76.
Ha-Kyung Kwon Vanessa E. Lopez Raleigh L. Davis So Youn KimAdam B. Burns Richard A. Register 《Polymer》2014
Anionic polymerization was employed to synthesize well-defined diblock copolymers of polystyrene and poly(2-ethylhexylmethacrylate), PS-PEHMA. Diblock morphologies in bulk and in substrate-supported thin films were characterized by small-angle X-ray scattering (SAXS) and atomic force microscopy (AFM), respectively. PS-PEHMA diblocks exhibited thermotropic order-disorder transitions; one diblock showed a thermoreversible transition between lamellae and a higher-temperature morphology assigned as perforated lamellae. Unlike PS-poly(alkylmethacrylate) diblocks where the alkyl group is n-butyl or n-pentyl, PS-PEHMA diblocks showed a typical decreasing Flory interaction parameter with increasing temperature. Thin films of PS-cylinder-forming PS-PEHMA diblocks showed a strong preference for the cylinders to lie in the plane of the film; films of incommensurate thickness readily formed terraces. Films of commensurate thickness were easily aligned over macroscopic areas through the application of mechanical shear. 相似文献
77.
Natural Abundance Carbon Isotopic Analysis Indicates the Equal Contribution of Local Synthesis and Plasma Uptake to Palmitate Levels in the Mouse Brain 下载免费PDF全文
R. J. Scott Lacombe Vanessa Giuliano Raphaël Chouinard‐Watkins Richard P. Bazinet 《Lipids》2018,53(5):481-490
Saturated fatty acids are the most abundant fatty acids in the brain, however, there has been some debate regarding the ability of intact dietary saturated fatty acids to be incorporated into the brain. In the present study, we use compound specific isotope analysis to measure the natural abundance carbon isotopic signature of brain, liver, and blood palmitic acid (PAM) and compare it to the dietary PAM and sugar isotopic signatures to calculate the relative contribution of both the incorporation of intact and endogenously synthesized PAM to these pools. Mice were equilibrated to the study diet, and extracted fatty acids were analyzed with gas chromatography isotope ratio mass spectrometry to determine the carbon isotopic signature of PAM (δ13CPAM). Liver, serum total, and serum unesterified fatty acid δ13CPAM ranged between ?20.6 and ?21.1 mUr and were approximately 8.5 mUr more enriched in 13C when compared to the dietary PAM signature. Brain δ13CPAM was found to be more enriched than liver or blood pools (?16.7 ± 0.2 mUr, mean ± SD). Two end‐member‐mixed modeling using the carbon isotopic signature of dietary PAM and dietary sugars determined the contribution of synthesis to the total tissue PAM pool to range between 44% and 48%. This suggests that endogenous synthesis and dietary PAM are near equal contributors to brain, liver, and blood PAM pools. In conclusion, our data provide evidence that brain PAM levels are maintained by both local endogenous synthesis and through the uptake of intact PAM from the blood. 相似文献
78.
Vanessa Schmidt Cristiano Giacomelli Alain R. Brisson Redouane Borsali 《Materials science & engineering. C, Materials for biological applications》2008,28(4):479-488
The formation of Annexin-A5 decorated (bio-functionalized) nanoparticles is of particular interest in micelle-mediated target drug delivery, in vivo magnetic resonance imaging, and controlled fabrication of biochips. This work describes an easy access to the synthesis and manipulation of block copolymer nano-objects exhibiting Annexin-A5 protein binding ability. Well-defined spherical micelles containing negatively charged phosphonic diacid groups – which are potential binding sites for Annexin-A5 proteins – at their hydrophilic periphery originate from the self-assembly of polystyrene-b-poly(2-phosphatethyl methacrylate-stat-2-hydroxyethyl methacrylate) (PS-b-P(PEMA-stat-HEMA)) amphiphilic macromolecules in aqueous media. PS-b-P(PEMA-stat-HEMA) can be prepared in a three-step phosphorylation/silylation/methanolysis procedure applied to PS-b-PHEMA precursors synthesized via Atom Transfer Radical Polymerization (ATRP). The herein discussed approach allows precise control over micellar dimensions and properties such as core radius (i.e., loading capacity), corona width, and density of phosphate groups at the micelle periphery. 相似文献
79.
Riparian plant communities are shaped by gradients of disturbance intensity and frequency and resource availability. Reservoir operation can alter the composition and abundance of riparian vegetation by changing the flood regime and by trapping fine sediments and associated nutrients within the reservoir system. We examined differences in herbaceous species richness, abundance and composition in Populus‐Salix stands along an unregulated and regulated reach of a river in semi‐arid Arizona, contrasted flood inundation frequency and edaphic conditions (soil moisture, nutrients and texture) between the reaches, and interpreted the vegetation differences in light of observed differences in environmental conditions. Flooding frequency was similar between reaches, but the proportion of fine textured soils in the unregulated reach was nearly double that of the regulated reach and soil nutrient levels were up to three times higher in the unregulated reach. Herbaceous cover and richness were consistently lower in the regulated reach, with between‐reach differences greatest during dry seasons. These patterns suggest that an edaphic‐based change in resource availability is the principal pathway by which river damming is altering herbaceous vegetation in this system. Our results demonstrate that sediment transport within riparian corridors is important for maintenance of herbaceous communities and that restoration of flow regimes alone may be insufficient to restore herbaceous flora on some regulated reaches. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
80.
Looking for evidence of learning: Assessment and analysis methods for online discourse 总被引:1,自引:0,他引:1
Discussion is a required component of many Web-based classes, but do we really know its value or contribution to learning? Students may be graded for participation, and number and length of posts may be counted by those evaluating or researching online classes, but all too often the assessment and analysis methods that we use fail to provide us with data that indicate learning took place through participation in online discussion. This paper explores what can be learned by currently popular assessment practices and analytic methods, as well as the limitations of these methods when used to measure learning, using two samples of discussion threads from online classes. 相似文献