Small ligands that neither bind to nor alter the structure of d(GA x TC)n sequences in DNA

Three minor-groove binding ligands have been used to study the characteristics of two d(GA x CT)n DNAs embedded in longer DNA fragments. The binding of mithramycin, netropsin or Thia-Net to these sequences has been studied using DNAse I footprinting. None of these ligands appeared to bind to d(GA x CT)5 nor to d(GA x CT)22 extensively, although with mithramycin some protected bonds were detected at the very edge of these sequences. In general, these small ligands did not enhance the DNAse I cleavage patterns at the alternating d(GA x CT)n flanking sequences located near DNA regions where the drug was bound. The d(GA x CT)n sequences could act as a rigid block in which it is not easy to propagate structural changes, whereas other sequences flanking the binding sites showed cleavage enhancements.     

Post-design analysis for building and refining AI planning systems

The growth of industrial applications of artificial intelligence has raised the need for design tools to aid in the conception and implementation of such complex systems. The design of automated planning systems faces several engineering challenges including the proper modeling of the domain knowledge: the creation of a model that represents the problem to be solved, the world that surrounds the system, and the ways the system can interact with and change the world in order to solve the problem. Knowledge modeling in AI planning is a hard task that involves acquiring the system requirements and making design decisions that can determine the behavior and performance of the resulting system. In this paper we investigate how knowledge acquired during a post-design phase of modeling can be used to improve the prospective model. A post-design framework is introduced which combines a knowledge engineering tool and a virtual prototyping environment for the analysis and simulation of plans. This framework demonstrates that post-design analysis supports the discovery of missing requirements and can guide the model refinement cycle. We present three case studies using benchmark domains and eight state-of-the-art planners. Our results demonstrate that significant improvements in plan quality and an increase in planning speed of up to three orders of magnitude can be achieved through a careful post-design process. We argue that such a process is critical for the deployment of AI planning technology in real-world engineering applications.     

A Phase‐Based Approach for Animating Images Using Video Examples

We present a novel approach for animating static images that contain objects that move in a subtle, stochastic fashion (e.g. rippling water, swaying trees, or flickering candles). To do this, our algorithm leverages example videos of similar objects, supplied by the user. Unlike previous approaches which estimate motion fields in the example video to transfer motion into the image, a process which is brittle and produces artefacts, we propose an Eulerian phase‐based approach which uses the phase information from the sample video to animate the static image. As is well known, phase variations in a signal relate naturally to the displacement of the signal via the Fourier Shift Theorem. To enable local and spatially varying motion analysis, we analyse phase changes in a complex steerable pyramid of the example video. These phase changes are then transferred to the corresponding spatial sub‐bands of the input image to animate it. We demonstrate that this simple, phase‐based approach for transferring small motion is more effective at animating still images than methods which rely on optical flow.     

Automated GIS-Based System for Speed Estimation and Highway Safety Evaluation

Although geographic information systems (GISs) have been applied in different areas of transportation, full use of their potential in speed profile analysis and highway design consistency studies has yet to be made. The possibilities that GISs offer for analysis and the increasing number of highway inventories supported by such systems, including traffic data and crash rates, suggest that the implementation of a highway design consistency analysis module within a GIS will facilitate and improve road safety studies. It is with this aim that a GIS-based computer system that builds vehicle speed profiles and analyzes highway design consistency has been developed, in which an operating speed prediction model, acceleration/deceleration rates, and other features can be configured by the engineering user in order to obtain an analysis that is more representative of drivers and local conditions. Application of this system to the study of the design consistency of highways is described, using three examples.     

Copper bioavailability in rats fed unused palm olein and palm olein used in frying

The influence of the consumption of unused and used (in repeated potato frying operations) palm olein on copper bioavailability was investigated. Three diets containing respectively 8% olive oil (O), palm olein (P) and palm olein from 80 repeated potato frying operation without turnover (PF), which nearly reached the limit of 25% polar compounds allowed by law, were given to rats for 28 days. No differences were found in food intake and body weight. The liver mass index was higher in PF than in P rats. No significant differences were observed in copper apparent absorption or in liver, skin, erythrocyte, serum and carcass copper contents. Therefore consumption of palm olein, whether unused or used in frying, does not alter copper bioavailability in growing rats. Copyright © 2004 Society of Chemical Industry     

Influence of the Reduction of Graphene Oxide with Hydroiodic Acid on the Structure and Photoactivity of CdS–rGO Hybrids

The temperature used in the chemical reduction of graphene oxide (GO) with hydroiodic acid has a significant influence on the removal of surface oxygenated functional groups, on the residual iodine species and on the rupture, stacking and graphitization of the graphene sheets in the reduced graphene oxides. The modification in the characteristics of the reduced graphene oxides induces changes in the surface area, the exposition of reduced graphene oxide entities and in the concentration of small CdS nanocrystals with strong confinement effect on the CdS-reduced graphene oxide hybrids. The hybridization of the reduced graphene oxide with CdS modifies in different way their photocatalytic behavior for hydrogen production from aqueous solutions of Na₂S and Na₂SO₃ under simulated sunlight irradiation. Only the hybrid formed between the CdS and the reduced graphene oxide treated at higher temperature showed improved hydrogen production rate respect to the bare CdS reference associated with the better conductivity of the reduced graphene oxide and with the increase in the concentration of small CdS nanocrystals sith strong confinement effect observed in the hybrid.     

Analysis of telomeric length in epithelial carcinoma of the ovary

BACKGROUND: As a consequence of the high cell division rate, telomeric repeat reduction in human tumor cells, giving rise to genetic instability, has recently been described. The aim of this study was to analyze by Southern blot telomeric length alterations in a retrospective group of patients with ovarian epithelial carcinoma. PATIENTS AND METHODS: Tumor and corresponding normal DNA were isolated from paraffin-embedded tissue of 16 patients with ovarian epithelial carcinoma. Telomeric Restriction Fragments (TRF) were studied by Southern blot and densitometric analysis. RESULTS: No telomere alterations were detected in 37.5% of patients (6/16). Of the remaining ten, 5 were found to have telomere reduction and five telomere elongation. No significant correlation was found between clinicopathological variables, response to chemotherapy, survival rate or time to progression, and telomere length alterations. CONCLUSIONS: In ovarian epithelial carcinoma telomere elongation may be a marker of the presence of immortal cells within the tumor, but telomere or the absence of telomeric alterations do not rule out the presence of these cells. Although TRF analysis can be performed in paraffin-embedded tissues, it is not the best indicator of telomerase activity and thus of tumor aggressiveness in early stages of this carcinoma.     

Influence of the reduction of graphene oxide (rGO) on the structure and photoactivity of CdS-rGO hybrid systems

Solvothermal and chemical reduction of graphene oxide with N₂H₄ or HI affect the surface composition, rupture and delamination degree of reduced graphene oxide (rGO). Higher reduction and stacking of rGO was achieved by chemical reduction with HI, while solvothermal reduction and, especially, the chemical reduction with N₂H₄ lead to higher delamination of rGO. The incorporation of the different rGO to CdS implies changes in the characteristics and photoactivity of the CdS-rGO hybrids. A promoter effect was observed in all CdS-rGO hybrids respect to the photoactivity of bare CdS, observing the better photoactivity on the hybrid in which the graphene oxide was reduced with HI (CdS-rGO/HI). The variations in the photoactivity of CdS-rGO hybrids are analyzed in terms of changes in the structure, surface and light absorption ability of CdS and also analyzing the contact of CdS with rGO. The greater concentration of small CdS nanostructures with strong quantum confinement is in the origin of the enhancement in photoactivity observed in the CdS-rGO/HI hybrid.     

Pyrrolo[1,2-a]quinoxalines: Insulin Mimetics that Exhibit Potent and Selective Inhibition against Protein Tyrosine Phosphatase 1B

PTP1B dephosphorylates insulin receptor and substrates to modulate glucose metabolism. This enzyme is a validated therapeutic target for type 2 diabetes, but no current drug candidates have completed clinical trials. Pyrrolo[1,2-a]quinoxalines substituted at positions C1–C4 and/or C7–C8 were found to be nontoxic to cells and good inhibitors in the low- to sub-micromolar range, with the 4-benzyl derivative being the most potent inhibitor (0.24 μm ). Some analogues bearing chlorine atoms at C7 and/or C8 kept potency and showed good selectivity compared to TCPTP (selectivity index >40). The most potent inhibitors behaved as insulin mimetics by increasing glucose uptake. The 4-benzyl derivative inhibited insulin receptor substrate 1 and AKT phosphorylation. Molecular docking and molecular dynamics simulations supported a putative binding mode for these compounds to the allosteric α3/α6/α7 pocket, but inconsistent results in enzyme inhibition kinetics were obtained due to the high tendency of these inhibitors to form stable aggregates. Computational calculations supported the druggability of inhibitors.