Carbohydrate moieties on the in vitro immunoreactivity of soy β-conglycinin

β-Conglycinin is a functional glycoprotein and one of the most important soybean allergens. The aim of the present research was to investigate the role of the N-glycans moieties of β-conglycinin on its in vitro immunoreactivity. The soy allergen was obtained by isoelectric precipitation from commercial soy protein isolate and was enzymatically deglycosylated by PNGase F (Peptide N-Glycosidase F EC 3.5.1.52). In order to optimize deglycosylation conditions different reaction times and allergen concentrations were tested. The extent of deglycosylation was estimated by SDS–PAGE, CZE, RP-HPLC, and MALDI-TOF MS analyses, which provided information related to changes in protein structure. The antigenicity of both native β-conglycinin and its deglycosylated form was evaluated by western-blotting and indirect ELISA employing polyclonal rabbit anti-soybean sera and horseradish peroxidase-labeled goat anti-rabbit IgG while the in vitro allergenicity was assessed by means of indirect competitive inhibition ELISA employing human sera (IgE) of soy allergics. β-Conglycinin was effectively deglycosylated by PNGase F. Data on immunological tests suggested that glycosyl moieties forming this glycoprotein might be involved in its immunoreactivity.     

A circulatory system approach for wireless sensor networks

One of the challenges in a military wireless sensor network is the determination of an information collection infrastructure which minimizes battery power consumption. The problem of determining the right information collection infrastructure can be viewed as a variation of the network design problem, with the additional constraints related to battery power minimization and redundancy. The problem in its generality is NP-hard and various heuristics have been developed over time to address various issues associated with it. In this paper, we propose a heuristic based on the mammalian circulatory system, which results in a better solution to the design problem than the state of the art alternatives.     

A regenerative multiple zone model for HCCI combustion

A new conserved scalar approach, the so-called regenerative multiple zone (RMZ) model, is introduced to simulate combustion in homogeneous charge compression ignition (HCCI) engines with significant products of combustion. In this approach, two conserved scalars are introduced, the mixture fraction Z and the initial exhaust gas fraction J, to determine uniquely the state of the reactive system as a function of the two conserved scalars and time. For the numerical solution of the HCCI combustion, the conserved scalar plane is divided into different zones, which represent homogeneous reactors with constant initial exhaust gas level. Particularly, the zones are created based on the distribution of the initial exhaust gases and are mixed and regenerated at every time step during combustion in order to account for the history effects which are due to the finite rate chemistry. A proper methodology to create and initialize the new zones during the combustion, the so-called zone creation strategy (ZCS), is also proposed. For validation, the RMZ model is implemented in the 2DRD code, which is a computational fluid dynamics code that solves the governing equations for a two-dimensional reaction-diffusion problem. Initially, the consistency of the new model is validated in a one-dimensional reaction-diffusion (RD) case. Subsequently, the necessity for a proper zone creation strategy is demonstrated by a two-dimensional RD case. Next, a parametric study is performed to investigate the sensitivity of the new model on the maximum number of zones that is used. Finally, the limitations as well as the advantages of the RMZ model are discussed.     

Choosing the right antibiotic

BACKGROUND: With the increasing complexity of the antibiotic armamentarium and the increasing prevalence of resistance, the choice of an appropriate antibiotic is more difficult than ever before. There is also increasing concern that overuse of broad spectrum antibiotics may help reduce their effectiveness in the future. In attempting to make rational antibiotic choices, competing interests must be balanced in order to preserve one of our most powerful medical tools. OBJECTIVE: The aim of this paper is to discuss some of the factors that should be considered when selecting an antibiotic and to provide general principles for use in day to day decision making. DISCUSSION: A knowledge of the patient combined with a working knowledge of the likely target organism and its predicted susceptibility profile is the basis of antibiotic selection.     

Comparative analysis of a twin-class M-QAM transmission system for wireless video applications

This paper presents a unique set of techniques to support reliable and efficient video transmission over mobile channels. The transmission system is comprised of an M level Quadrature Amplitude Modulation (QAM) technique. A twin class uniform and non-uniform partitioned M-QAM system is used to transport a compressed video bitstream which is partitioned to match the bit-error sensitivity of the transmitted symbol in terms of mapping in the constellation diagram and picture quality. Video partitioning based on a separation of the Variable Length Coded (VLC) Discrete Cosine Transforms (DCT) coefficients within each block is considered for constant bitrate transmission (CBR). Various scenarios for splitting the bitstream are investigated and their results are compared and analysed thoroughly. The performance of the transmission system is evaluated under Additive White Gaussian Noise (AWGN) conditions. The simulation results showed that the video partition strategy results in a significantly higher quality of the reconstructed video data.     

Calprotectin and Imbalances between Acute-Phase Mediators Are Associated with Critical Illness in COVID-19

The trajectory from moderate and severe COVID-19 into acute respiratory distress syndrome (ARDS) necessitating mechanical ventilation (MV) is a field of active research. We determined serum levels within 24 h of presentation of 20 different sets of mediators (calprotectin, pro- and anti-inflammatory cytokines, interferons) of patients with COVID-19 at different stages of severity (asymptomatic, moderate, severe and ARDS/MV). The primary endpoint was to define associations with critical illness, and the secondary endpoint was to identify the pathways associated with mortality. Results were validated in serial measurements of mediators among participants of the SAVE-MORE trial. Levels of the proinflammatory interleukin (IL)-8, IL-18, matrix metalloproteinase-9, platelet-derived growth factor (PDGF)-B and calprotectin (S100A8/A9) were significantly higher in patients with ARDS and MV. Levels of the anti-inflammatory IL-1ra and IL-33r were also increased; IL-38 was increased only in asymptomatic patients but significantly decreased in the more severe cases. Multivariate ordinal regression showed that pathways of IL-6, IL-33 and calprotectin were associated with significant probability for worse outcome. Calprotectin was serially increased from baseline among patients who progressed to ARDS and MV. Further research is needed to decipher the significance of these findings compared to other acute-phase reactants, such as C-reactive protein (CRP) or ferritin, for the prognosis and development of effective treatments.     

Effect of standard phenolic compounds and olive oil phenolic extracts on acrylamide formation in an emulsion system

In this work the effect of standard phenolic compounds such as trolox, phenolic acids (ferulic, gallic, protocatechuic and caffeic acid) and secoiridoids, extracted from virgin olive oil (VOO), on acrylamide formation in an emulsion system was investigated. The emulsion system was heated in an oil bath at different temperatures and for different periods of time. At 105 and 115 °C the generated amounts of acrylamide were negligible during the monitoring time. At 125 °C addition of standard phenolic compounds reduced acrylamide formation which was more pronounced at initial time of heating (20 min) reaching up to 70%. Protocatechuic and gallic acid were more effective than trolox, caffeic and ferulic acid in acrylamide reduction at the final time intervals of heating (30–60 min). On the contrary, addition of VOO extract in the emulsion system resulted in a remarkable increase of acrylamide level, which was ascribed to secoiridoids’ structure containing aldehydic groups.