Bioprocess Scale-up for Acetohydroxamic Acid Production by Hyperactive Acyltransferase of Immobilized Rhodococcus Pyridinivorans

Catalysis Letters - In this study, Rhodococcus pyridinivorans cells containing hyperactive acyltransferase was immobilized on various macromolecules based-polymeric matrices and used to improve...     

Effects of Post-Weld Heat Treatment on the Microstructure and Toughness of Flash Butt Welded High-Strength Low-Alloy Steel

Metallurgical and Materials Transactions A - Effect of post-weld heat treatment on the weld microstructure, texture, and its correlation to the toughness of flash butt welded joints were...     

IPS-MAC: an informative preamble sampling MAC protocol for wireless sensor networks

Radio transceivers are the main source of energy consumption in wireless sensor networks (WSNs) where the source of energy supply is non-rechargeable battery. Several MAC protocols have been proposed in order to efficiently conserve energy in the link layer via duty-cycling. Low power listening (LPL) methods have been shown to outperform other schemes in lightly loaded situations which are common in environment monitoring applications. Nonetheless, as the network becomes dense, in LPL protocols such as BMAC a large number of nodes stay awake for each transmission, resulting in high levels of energy consumption. This paper introduces the informative preamble sampling (IPS) protocol in which a transmitter implicitly embeds information about its intended receiver via the power at which the preamble is transmitted. This results in far fewer nodes staying awake for each preamble. Upon hearing the preamble, a receiver executes a decision-making algorithm to decide whether to stay awake. If the decision-making algorithm is too lax, then more nodes stay awake following the preamble. On the other hand if the algorithm is too strict, it is likely that the intended receiver misses the preamble. In this paper we derive the optimal operating points for the IPS protocol. We show analytically that the IPS protocol can achieve a gain in energy by at least a factor of 2 over BMAC. We also conduct extensive simulations to show that IPS can achieve significant energy gains compared to BMAC.     

Enhanced electrocatalytic performance of cyano groups containing conducting polymer supported catalyst for oxidation of formic acid

A new palladium (Pd) based catalyst was developed using poly(diphenylamine-co-3-aminobenzonitrile) (P(DPA-co-3ABN)) as the new catalyst support. The sizes, distribution and stability of Pd nanoparticles (Pd NPs) are strongly influenced by the cyano group (–CN) present in P(DPA-co-3ABN). Field emission scanning electron microscopy image and energy dispersive x-ray analysis revealed good dispersion of Pd NP onto P(DPA-co-3ABN) matrix. The electrocatalytic activity of P(DPA-co-3ABN)/Pd catalyst electrode (CE) was investigated in terms of formic acid (FA) electro oxidation. The onset potential and catalytic current for the electro oxidation of FA are higher at P(DPA-co-3ABN)/Pd-CE as compared to PdNPs loaded pristine PDPA catalyst electrode (PDPA/Pd-CE). P(DPA-co-3ABN)/Pd-CE exhibited 18 time higher electrocatalytic current than PDPA/Pd-CE for oxidation of FA.     

ARL1 and membrane traffic in Saccharomyces cerevisiae

To examine the functions of the Arf-like protein, Arl1p, in Saccharomyces cerevisiae, a null allele, arl1delta::HIS3, was constructed in two strains. In one background only, loss of ARL1 resulted in temperature-sensitive (ts) growth (suppressed on high-osmolarity media). Allelic variation at the SSD1 locus accounted for differences between strains. Strains lacking ARL1 exhibited several defects in membrane traffic. First, arl1delta strains secreted less protein as measured by TCA-precipitable radioactivity found in the media of [(35)S]-labelled cells. A portion of newly synthesized carboxypeptidase Y (CPY) was secreted rather than correctly targeted to the vacuole. Uptake of the fluid-phase marker, lucifer yellow, was reduced. All these phenotypes were exacerbated in an ssd1 background. The ts phenotype of the arl1deltassd1 strain was suppressed by YPT1, the yeast Rab1a homologue, suggesting that ARL1 and YPT1 have partially overlapping functions. These findings demonstrate that ARL1 encodes a regulator of membrane traffic.     

Emergent properties of networks of biological signaling pathways

Many distinct signaling pathways allow the cell to receive, process, and respond to information. Often, components of different pathways interact, resulting in signaling networks. Biochemical signaling networks were constructed with experimentally obtained constants and analyzed by computational methods to understand their role in complex biological processes. These networks exhibit emergent properties such as integration of signals across multiple time scales, generation of distinct outputs depending on input strength and duration, and self-sustaining feedback loops. Feedback can result in bistable behavior with discrete steady-state activities, well-defined input thresholds for transition between states and prolonged signal output, and signal modulation in response to transient stimuli. These properties of signaling networks raise the possibility that information for "learned behavior" of biological systems may be stored within intracellular biochemical reactions that comprise signaling pathways.     

Huffman encoding of test sets for sequential circuits

Sequential circuits are hard to test because they contain a large number of internal states that are difficult to control and observe. Scan design is often used to simplify testing; however, scan is not always applicable because of area and performance penalties. Recent advances in sequential circuit testing have led to techniques and tools that provide test sets with high coverage of single stuck-line (SSL) faults for nonscan circuits. However, these test sets contain a large number of patterns and require a tester with considerable pattern depth. We investigate the application of Huffman codes to pattern encoding. This allows the use of low-cost testers that do not require excessive memory. Our method is especially applicable to nonscan and partial-scan embedded core circuits. We demonstrate the feasibility of our approach by applying it to SSL test sets for the ISCAS'89 benchmarks     

Gbeta subunit interacts with a peptide encoding region 956-982 of adenylyl cyclase 2. Cross-linking of the peptide to free Gbetagamma but not the heterotrimer

The region encoded by amino acids 956-982 of adenylyl cyclase 2 is important for Gbetagamma stimulation. Interactions of a peptide encoding the 956-982 region of adenylyl cyclase 2 (QEHAQEPERQYMHIGTMVEFAYALVGK (QEHA peptide)) with Gbetagamma subunits were studied. QEHA peptide was covalently attached to beta subunit of free Gbetagamma by the cross-linker N-succinimidyl(4-iodoacetyl)aminobenzoate. Cross-linking was proportional to the amount of QEHA peptide added; other control peptides cross-linked minimally. When Go was used, very little cross-linking was observed with GDP and EDTA, but upon activation by guanosine 5'-3-O-(thio)triphosphate and Mg2+, specific cross-linking of the QEHA peptide to Gbeta was observed. We conclude that beta subunits of G proteins contain effector interaction domains that are occluded by Galpha subunits in the heterotrimer. Molecular modeling studies used to dock the QEHA peptide on to Gbeta indicate that amino acids 75-165 of Gbeta may be involved in effector interactions.     

Vapour phase crystal growth under microgravity environment

Vapour phase crystal growth experiments performed in the Skylab and ASTP missions are reviewed. The basic vapour phase crystal growth technique is described and effect of gravity is discussed. The multipurpose furnace specially designed to carry out various experiments in flight conditions is described. Ge Se, Ge Te and GeS as well as ternary GeSe_0·99 Te_0·01 and GeS_0·98 Se_0·02 crystals have been grown in space showing improvement over similarly grown crystals on ground as determined by x-ray diffraction, chomical homogeneity and surface morphology studies. Mass flux rates under microgravity conditions have been found to be up to 10 times larger than expected indicating need for better theoretical and experimental understanding of the effect of gravity on crystal growth.