The stability and unfolding of an IgG binding protein based upon the B domain of protein A from Staphylococcus aureus probed by tryptophan substitution and fluorescence spectroscopy

The stability and unfolding of an immunoglobulin (Ig) G bindingprotein based upon the B domain of protein A (SpAB) from Staphylococcusaureus were studied by substituting tryptophan residues at strategiclocations within each of the three a-helical regions (al-a3)of the domain. The role of the C-terminal helix, a3, was investigatedby generating two protein constructs, one corresponding to thecomplete SpAB, the other lacking a part of ct3; the Trp substitutionswere made in both one-and two-domain versions of each of theseconstructs. The fluorescence properties of each of the single-tryptophanmutants were studied in the native state and as a function ofguanidine-HCl-mediated unfolding, and their IgG binding activitieswere determined by a competitive enzyme-linked immunosorbentassay. The free energies of folding and of binding to IgG foreach mutant were compared with those for the native domains.The effect of each substitution upon the overall structure andupon the IgG binding interface was modelled by molecular graphicsand energy minimization. These studies indicate that (i) 3 contributesto the overall stability of the domain and to the formationof the IgG binding site in l and 2, and (ii) al unfolds first,followed by 2 and 3 together.     

Adhesion-Delamination Mechanics of a Prestressed Circular Film Adhered onto a Rigid Substrate

A thin circular film clamped at the periphery is adhered to the planar surface of a rigid cylindrical punch. An external tensile load is applied to the punch, causing the film to delaminate from the substrate and the circular contact edge to contract. The film spontaneously separates from the punch, or pulls off, when the contact radius reduces to a range between 0.1758 and 0.3651 of the film radius, depending on the magnitude of the residual membrane stress. The mechanical delamination process is derived by a thermodynamic energy balance based on a coupled interfacial adhesion and residual membrane stress. The theoretical model has significant implications in nanoforce measurement, microelectromechanical systems (MEMS) comprising active moveable films, and biological cell adhesion.     

Novel nitrogen‐containing epoxy resin. II. Cure kinetics by differential scanning calorimetry

The isothermal and nonisothermal cure behaviors of a novel nitrogen‐containing epoxy resin (XT resin) were studied by differential scanning calorimetry (DSC). Various kinetic parameters and details of cure process were obtained based on the Avrami theory. The results indicated that Avrami method is suitable for calculating the kinetic parameters up to the gel point at least. The apparent activation energy (E_a) for isothermal cure process was in agreement with that for nonisothermal cure process. E_a value in the early stage (78.5–81.0 KJ mol^?1) was about three times than that in the later stage (23.3–26.5 KJ mol^?1). The kinetic results from Avrami theory may present a combined effect of all factors, and which is helpful to understand the cure technique for XT–DDS system. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 3483–3489, 2006     

Charge/discharge characteristics of sulfur composite cathode materials in rechargeable lithium batteries

The charge and discharge characteristics of lithium batteries with sulfur composite cathodes have been investigated. The sulfur composites showed novel electrochemical characteristics. The analysis of the differential capacity indicated that the discharge process showed two voltage plateaus of 2.10 V and 1.88 V, and the charge process also presented two voltage plateaus of 2.22 V and 2.36 V. The overcharge test showed that the composite cannot be charged over 4.0 V, the voltage always stopped at about 3.9 V during charging, indicating that the composite presented the intrinsic safety for the overcharge of lithium batteries. The overcharge can cause serious safety problem for the conventional Li-ion batteries. The overcharge test also showed that the batteries with sulfur composite were destroyed when the upper cut-off voltage was over 3.6 V. However, the composite presented good reversible capacity after it was deep discharged even to 0 V. It showed stable cycleability and high cycling capacity of 1000 mAh g⁻¹ when cycling between 0.1 V and 3.0 V, indicative of the different characteristic from the conventional oxide cathode materials. The prototype polymer battery with the composite cathode material presented the energy density of 246 Wh kg⁻¹ and 401 Wh L⁻¹.     

探索新形势下军民融合式装备保障发展之路

从分析军民融合式装备保障的动因和存在的问题入手,阐述了构建完善军民融合式装备保障体系,必须要在思想观念、法规制度、体制机制、两用标准、军地人才、军用技术和民用技术等6个方面实现融合,为理解、贯彻装备保障走好军民融合式发展之路提供了一些思路和设想。     

抗日战争时期的青年心理探析

抗日战争是中华民族历史上的一次大浩劫,中国人民为此承受了太多的痛苦与磨难,特别是对于肩负着国家与民族振兴使命的青年更是如此.分析战时青年的心理变化及原因,指出政府和社会对青年人的心理没有采取恰当的方法进行有效的疏导,提出要总结和吸取历史的经验教训,为我们今天应对大灾难提供借鉴.     

浅谈土木工程专业毕业设计选题

结合土木工程专业毕业设计的具体情况,分析了毕业设计教学选题环节存在的问题,提出了相应的改进措施.     

不定积分中的几何背景和表格分部积分法

论述了数学背景对数学发展的重要性,提供了不定积分中的几何背景,介绍了不定积分的表格分部积分法及案例,对改革教学内容和改进教学方法进行了尝试。     

移动支付安全协议的研究

文章分析了在WAP环境下基于SET协议的移动支付的交易流程不满足商品原子性和确认发送原子性。当商家得到正确支付后,SET协议不能保证他一定会发货给持卡者,也不能保证发送的就是持卡者订购的商品。同时基于SET协议的安全性和不可否认性也存在着不足。基于这些局限性,本文通过对协议的改进,完善了移动支付的整个交易过程,切实保护了各方的利益。     

基于流固耦合的叶片动力特性分析

在考虑耦合流场与旋转预应力条件下,建立了对应的叶片流固耦合系统的动力特性方程.采用ANSYS与FLUENT对整体叶片的耦合模态进行了计算,并利用实验获得气流压力脉动数据,对叶片振动进行了瞬态分析.结果表明:在旋转预应力与耦合流场压力作用下,叶片模态频率约有9Hz的上升,模态振型的最大位移位置点转移,振动方向改变,前三阶相对振幅分别从29.128,19.400,44.566下降到28.945,19.285,44.562;叶片最大变形和加速度位于前缘的上端,最大应力点集中在叶片根部一侧.