Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences

The white-backed planthopper (WBPH), Sogatella furcifera, is one of the most important piercing-sucking pests of rice (Oryza sativa) in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH injected with dsSFMLP had difficulty in sucking phloem sap from rice plants, which significantly reduced their food intake, weight, and survival. In contrast, the knockdown of the SFMLP gene had only a marginal effect on the survival of WBPH fed an artificial diet. Further studies showed that silencing SFMLP resulted in the short and single-branched salivary sheaths secretion and less formation of salivary flanges in rice. These data suggest that SFMLP is involved in the formation of the salivary sheath and is essential for feeding in WBPH. Overexpression of the SFMLP gene in rice plants promoted the feeding of WBPH, whereas silencing the gene in rice plants significantly decreased WBPH performance. Additionally, it was found that overexpression of SFMLP in rice plants elicited the signalling pathway of SA (salicylic acid) while suppressing JA (jasmonic acid); in contrast, silencing of the SFMLP gene in rice plants showed the opposite results. This study clarified the function of SFMLP in WBPH feeding as well as mediating rice defences.     

新型耐盐耐溶剂水包油型高分子乳化剂C-5的合成及应用研究

以失水山梨醇单油酸酯(Span-80)作为乳化剂,偶氮二异丁基脒盐酸盐作为聚合引发剂,以丙烯酸(AA)、丙烯酰胺(AM)、二甲基二烯丙基氯化铵(DADMAC)和特殊单体为反应物,通过反相乳液聚合法合成了一种新型水包油型高分子乳化剂C-5,并考察了这种乳化剂的耐盐耐溶剂性能.     

Decode‐and‐forward with full‐duplex relaying

This paper addresses the full‐duplex relaying. Some expressions for outage and average capacity of a two‐hop cooperative system with a full‐duplex relay are derived under an independent but not identically distributed Rayleigh fading environment. Using these expressions, we provide the performance analysis without Monte Carlo simulations. The impact of interference between the relay output and input is investigated. Copyright © 2011 John Wiley & Sons, Ltd.     

常熟华府世家箱形转换层结构设计

常熟华府世家工程是一栋高层建筑,由于在转换梁的布置中有多次梁抬墙的情况,采用整体性好的箱形转换结构。对箱形转换层及其上下若干楼层的受力进行了计算,根据计算结果对箱形转换层进行结构设计。计算结果表明,采用箱形转换层提高了结构的整体性和抗震性能,结构的变形满足规范要求。对箱形转换结构的设计和施工中要注意的问题进行了探讨。     

Sulfated Hyaluronan Binds to Heparanase and Blocks Its Enzymatic and Cellular Actions in Carcinoma Cells

We examined whether sulfated hyaluronan exerts inhibitory effects on enzymatic and biological actions of heparanase, a sole endo-beta-glucuronidase implicated in cancer malignancy and inflammation. Degradation of heparan sulfate by human and mouse heparanase was inhibited by sulfated hyaluronan. In particular, high-sulfated hyaluronan modified with approximately 2.5 sulfate groups per disaccharide unit effectively inhibited the enzymatic activity at a lower concentration than heparin. Human and mouse heparanase bound to immobilized sulfated hyaluronan. Invasion of heparanase-positive colon-26 cells and 4T1 cells under 3D culture conditions was significantly suppressed in the presence of high-sulfated hyaluronan. Heparanase-induced release of CCL2 from colon-26 cells was suppressed in the presence of sulfated hyaluronan via blocking of cell surface binding and subsequent intracellular NF-κB-dependent signaling. The inhibitory effect of sulfated hyaluronan is likely due to competitive binding to the heparanase molecule, which antagonizes the heparanase-substrate interaction. Fragment molecular orbital calculation revealed a strong binding of sulfated hyaluronan tetrasaccharide to the heparanase molecule based on electrostatic interactions, particularly characterized by interactions of (−1)- and (−2)-positioned sulfated sugar residues with basic amino acid residues composing the heparin-binding domain-1 of heparanase. These results propose a relevance for sulfated hyaluronan in the blocking of heparanase-mediated enzymatic and cellular actions.     

"二墙合一"中的地下连续墙接缝的防渗与止水施工

在地下连续墙兼作地下结构外墙的“二墙合一”工程中,为防地下连续墙接缝处的渗漏,采用三重管高压摆喷工艺和压密注浆进行防渗处理,取得了理想的效果。     

NMR Characterization of Long-Chain Fatty Acylcarnitine Binding to the Mitochondrial Carnitine/Acylcarnitine Carrier

The mitochondrial carnitine/acylcarnitine carrier (CAC) transports short-, medium- and long-carbon chain acylcarnitines across the mitochondrial inner membrane in exchange for carnitine. How CAC recognizes the substrates with various fatty acyl groups, especially long-chain fatty acyl groups, remains unclear. Here, using nuclear magnetic resonance (NMR) technology, we have shown that the CAC protein reconstituted into a micelle system exhibits a typical six transmembrane structure of the mitochondrial carrier family. The chemical shift perturbation patterns of different fatty acylcarnitines suggested that the segment A76–G81 in CAC specifically responds to the long-chain fatty acylcarnitine. Molecular dynamics (MD) simulations of palmitoyl-L-carnitine inside the CAC channel showed the respective interaction and motion of the long-chain acylcarnitine in CAC at the cytosol-open state and matrix-open state. Our data provided a molecular-based understanding of CAC structure and transport mechanism.     

粮油中转储备库的加固改造设计

介绍了加固方案的选择、相关构件强度的计算、接点构造的处理等主要加固步骤。实践证明,此方案较经济、合理,且能最大程度地减少施工工作量。     

四川盆西平原区裸土扬尘中Pb、Cd化学形态的生物有效性

为探究四川盆西平原区裸土扬尘中Pb、Cd总质量分数与其生物有效性的规律,在构建的"阳离子型污染物土壤表层运移行为研究系统"内进行模拟起尘试验,对土壤样品进行Pb、Cd模拟污染试验,结果表明:试验条件下,四川盆西平原区裸土扬尘颗粒物中Pb、Cd的总质量分数随着粒径的减小而增大,其中Pb的化学形态以碳酸盐结合态为主(44....     

600MW空冷机组锅炉烟风道优化设计分析

针对600 MW空冷机组原烟风道占地面积大,材料用量多、造价高的问题,结合河北建投沙河发电有限公司2台600 MW超临界空冷机组工程中烟风道的设计,比较分析烟风道采用常规矩形道体和圆形道体的优劣,认为烟风道道体优化形状为圆形,介绍除尘器进口烟道、热一次风道、引风机进口烟道优化布置方案,并进行经济性分析。