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81.
Enhanced and Differential Capture of Circulating Tumor Cells from Lung Cancer Patients by Microfluidic Assays Using Aptamer Cocktail 下载免费PDF全文
Libo Zhao Chuanhao Tang Li Xu Zhen Zhang Xiaoyan Li Haixu Hu Si Cheng Wei Zhou Mengfei Huang Anna Fong Bing Liu Hsian‐Rong Tseng Hongjun Gao Yi Liu Xiaohong Fang 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(8):1072-1081
Collecting circulating tumor cells (CTCs) shed from solid tumor through a minimally invasive approach provides an opportunity to solve a long‐standing oncology problem, the real‐time monitoring of tumor state and analysis of tumor heterogeneity. However, efficient capture and detection of CTCs with diverse phenotypes is still challenging. In this work, a microfluidic assay is developed using the rationally‐designed aptamer cocktails with synergistic effect. Enhanced and differential capture of CTCs for nonsmall cell lung cancer (NSCLC) patients is achieved. It is also demonstrated that the overall consideration of CTC counts obtained by multiple aptamer combinations can provide more comprehensive information in treatment monitoring. 相似文献
82.
有限元后处理中超收敛计算的EEP(单元能量投影)法以及基于该法的自适应分析方法对线性ODE(常微分方程)问题的求解已经获得了全面成功,也推动了非线性ODE问题自适应求解的研究。经过研究,已经实现了一维有限元自适应分析技术从线性到非线性的跨越,该文意在对这方面的进展作一简要综述与报道。该文提出一种基于EEP法的一维非线性有限元自适应求解方法,其基本思想是通过线性化,将现有的线性问题自适应求解方法直接引入非线性问题求解,而无需单独建立非线性问题的超收敛计算公式和自适应算法,从而构成一个统一的、通用的非线性问题自适应求解算法。该文给出的数值算例表明所提出的算法高效、稳定、通用、可靠,解答可逐点按最大模度量满足用户给定的误差限,可作为先进高效的非线性ODE求解器的核心理论和算法。 相似文献
83.
Dong Wu Si‐Zhu Wu Shuai Zhao Jia Yao Jiang‐Nan Wang Qi‐Dai Chen Hong‐Bo Sun 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(5):760-767
A simple strategy to realize new controllable 3D microstructures and a novel method to reversibly trapping and releasing microparticles are reported. This technique controls the height, shape, width, and arrangement of pillar arrays and realizes a series of special microstructures from 2‐pillar‐cell to 12 cell arrays, S‐shape, chain‐shape and triangle 3‐cell arrays by a combined top down/bottom up method: laser interference lithography and capillary force‐induced assembly. Due to the inherent features of this method, the whole time is less than 3 min and the fabricated area determined by the size of the laser beam can reach as much as 1 cm2, which shows this method is very simple, rapid, and high‐throughput. It is further demonstrated that the ‘mechanical hand’‐like 4‐cell arrays could be used to selectively trap/release microparticles with different sizes, e.g., 1.5, 2, or 3.5 μm, which are controlled by the period of the microstructures from 2.5 to 4 μm, and 6 μm. Finally, the ‘mechanical hand’‐like 4‐cell arrays are integrated into 100 μm‐width microfluidic channels prepared by ultraviolet photolithography, which shows that this technique is compatible with conventional microfabrication methods for on‐chip applications. 相似文献
84.
Ruilian Zhang Guoqing Shi Yuanni Wang Si Zhao Shafiq Ahmad Xiaochen Zhang 《影响评估与项目评价》2018,36(4):331-347
In order to analyze potential social risks, and achieve smooth implementation of investment activities in the China–Pakistan economic corridor (CPEC), we attempt to establish social impact and risk indicators. Both the impacts on the projects and the impacts of the projects are used to develop risk indicators in accordance with the literature review, reality and international standard principles. Using an analytic hierarchy process (AHP) and average value to analyze questionnaires of both Chinese and Pakistani respondents, we identify the importance of various risk indicators. The primary social risks of the western high mountain region include tribal obstacles and religious extremism. The social risks to the southeast coastal plains include preserving the historical and cultural heritage of the area and international protection of national parks. The social risks in north Kashmir include disputes, extremist threats, religious and cultural differences, and the protection of natural reserves. The social risks of the Xinjiang region primarily involve social issues faced by ethnic minorities and environmental pressures. 相似文献
85.
RA Barton IR Storch VP Adiga R Sakakibara BR Cipriany B Ilic SP Wang P Ong PL McEuen JM Parpia HG Craighead 《Nano letters》2012,12(9):4681-4686
By virtue of their low mass and stiffness, atomically thin mechanical resonators are attractive candidates for use in optomechanics. Here, we demonstrate photothermal back-action in a graphene mechanical resonator comprising one end of a Fabry-Perot cavity. As a demonstration of the utility of this effect, we show that a continuous wave laser can be used to cool a graphene vibrational mode or to power a graphene-based tunable frequency oscillator. Owing to graphene's high thermal conductivity and optical absorption, photothermal optomechanics is efficient in graphene and could ultimately enable laser cooling to the quantum ground state or applications such as photonic signal processing. 相似文献
86.
87.
针对开放分布式实时系统中自治实体的交互行为和实时约束行为动态变化的问题,利用关注点分离思想,设计了一个具有实时与交互适应性的事件驱动协同模型.将传统自治实体分离成三种相对独立的实体:时控协同器、交互协同器和纯的计算实体.最后,利用Java消息服务和EJB技术给出了详细的实现构架,并通过一个车辆导航系统的实例说明这个构架如何取得交互和实时的适应性和灵活性. 相似文献
88.
Platinum catalyst for CO oxidation has been studied for decades,due to its high activity and good stability.In this work,we prepared three different lantha num oxide or hydroxide supports(LaOx(OH)y),and deposited platinum(Pt) with 0.5 at% via an impregnation approach to synthesize Pt/LaOx(OH)y catalysts.However,we find that these catalysts perform a poor stability for the CO oxidation reaction.The fresh and used samples were comprehensively characterized by multiple techniques including power X-ray diffraction(XRD),X-ray absorption fine structure(XAFS),transmission electron microscopy(TEM),temperature-programmed reduction by carbon monoxide(CO-TPR) and thermogravimetric analysis(TGA),to demonstrate that the oxidized platinum atoms or clusters,without any component of Pt-Pt metallic bond,are highly dispersed on the surface of LaOx(OH)y.Furthermore,the as-formed lanthanum carbonate(La2O2CO3) during the exposure to ambient circumstances or in the reaction atmosphere of CO+O2,severely impair the reactivity of Pt/LaOx(OH)y.On the basis of the obtained experimental results,we have drawn a conclusion that the oxidized PtOx atoms or PtxOy clusters are the active species for CO oxidation,while the formation of lanthanum carbonate is the origin of deactivation on reactivity. 相似文献
89.
目的 了解我国居民栀子食用情况,评估我国栀子食用人群栀子苷暴露水平及健康风险。方法 通过文献检索和专项监测收集我国栀子中栀子苷含量数据,结合2019—2022年我国食药物质消费量调查数据,采用简单分布方法和概率评估方法获得我国不同人群通过食用栀子的栀子苷暴露水平及健康风险。结果 我国9省栀子食用率为10.32%,每日食用量均值为0.36 g,高消费人群每日栀子食用量为1.48 g。简单分布评估显示,9省栀子食用人群的栀子苷每日平均暴露量为0.29 mg/kg·BW,高消费人群(P95)栀子苷暴露量为1.28 mg/kg·BW,9省栀子食用人群中有17.20%的居民通过食用栀子的栀子苷暴露量超过其每日可耐受摄入量(TDI,0.386 mg/kg·BW)。河南省食用人群每日平均暴露量最高,为0.54 mg/kg·BW。概率评估结果显示,我国14.70%的栀子食用人群栀子苷暴露量超过TDI,具有潜在健康风险。结论 我国栀子食用人群的栀子苷暴露风险总体较低,但部分高消费人群(P95)存在健康风险。 相似文献
90.
Yuanjie Li Jincheng Dai Zhongwei Si Kai Niu Chao Dong Jiaru Lin Sen Wang Yifei Yuan 《中国通信》2022,(3):70-87
Unsourced multiple access(UMA) is a multi-access technology for massive, low-power, uncoordinated, and unsourced Machine Type Communication(MTC) networks. It ensures transmission reliability under the premise of high energy efficiency.Based on the analysis of the 6G MTC key performance indicators(KPIs) and scenario characteristics,this paper summarizes its requirements for radio access networks. Following this, the existing multiple access models are analyzed under these standards to determine U... 相似文献