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871.
A Kokkevi J Liappas V Boukouvala V Alevizou E Anastassopoulou C Stefanis 《Canadian Metallurgical Quarterly》1993,31(2):111-121
In order to produce mAbs directed specifically against HLA-DR10 molecule, transfected mouse L cells, expressing the DRB1*1001 allele, were used to immunize C3H mice over a period of 4 weeks. Two mAbs, 2C12 and 4B6, derived from this fusion were found to recognize, with different affinity, polymorphic epitopes of DR10 that are shared with DR1, 3, 7, and 9. These mAbs were screened on a large panel of homozygous B lymphoblastoid cell lines using microlymphocytotoxicity and the results were confirmed by flow cytometry. The reactive pattern of 2C12 and 4B6 was compared to that of MP10 human mAb also recognizing the DR10 specificity in addition to DR1, 2 and 9. Based on serologic specificity and cellular absorption experiments, we conclude that the epitopes the murine and human mAbs respectively recognize on the DR10 molecule, are probably different. 相似文献
872.
R Mott A Grigoriev E Maier J Hoheisel H Lehrach 《Canadian Metallurgical Quarterly》1993,21(8):1965-1974
A complete set of software tools to aid the physical mapping of a genome has been developed and successfully applied to the genomic mapping of the fission yeast Schizosaccharomyces pombe. Two approaches were used for ordering single-copy hybridisation probes: one was based on the simulated annealing algorithm to order all probes, and another on inferring the minimum-spanning subset of the probes using a heuristic filtering procedure. Both algorithms produced almost identical maps, with minor differences in the order of repetitive probes and those having identical hybridisation patterns. A separate algorithm fitted the clones to the established probe order. Approaches for handling experimental noise and repetitive elements are discussed. In addition to these programs and the database management software, tools for visualizing and editing the data are described. The issues of combining the information from different libraries are addressed. Also, ways of handling multiple-copy probes and non-hybridisation data are discussed. 相似文献
873.
874.
G Biscaldi G Vittadini R Fonte P Gabanelli R Maglio E Finozzi 《Canadian Metallurgical Quarterly》1995,17(1-6):77-82
The relationship between alcohol and work was studied in a group of 537 voluntary patients (393 male and 144 female) admitted to the department of Medicina del Lavoro, Pavia University for alcohol rehabilitation. The highest incidence of alcohol abuse was found in non-working subjects (pensioners, unemployed, housewives) although the start of excessive drinking takes place after beginning a working activity; a cause-effect relationship between these two factors can not be clearly determined, however. It is true that the stress provoked by this new condition (introduction to the work experience) can, in certain subjects, be a contributing factor in alcohol abuse. The increasingly lower age of initiation to alcohol reconfirms the need for prevention and instruction even at scholastic level. We can deduce how no working condition, low age and low education can constitute makers of risk for alcohol abuse. 相似文献
875.
Rahman M.A. Osheiba A.M. Radwan T.S. Abdin E.S. 《Energy Conversion, IEEE Transaction on》1996,11(2):324-330
A method to analyze the steady-state performance of a stand-alone permanent magnet synchronous generator driven by a diesel engine is presented. The proposed method is based on equivalent d-q circuits and the phasor diagram of such a generator under steady-state conditions. A fixed capacitor-thyristor controlled reactor scheme is used to regulate the generator terminal voltage by controlling the thyristor ignition angle. Furthermore the overall system dynamics are modelled in terms of state variables and control inputs. Based on a reduced order linearized model, digital optimal state and output feedback controllers are designed by minimising a quadratic performance index using the dynamic programming technique. The objective of the controller is to maintain the load voltage and frequency constant under varying load conditions. The controller's effectiveness is assessed by examining the closed-loop system response to sudden load changes 相似文献
876.
The performance and architecture of a high dynamic range camera (HDRC) chip and the conceptional advantages for its adaptation to image processing systems in traffic environments are discussed. The HDRC chip was developed with 64×64 pixels using a standard digital 1.2-μm CMOS technology. It is shown that the implementation proves the functionality and indicates the system performance of a highly dynamic range camera is feasible 相似文献
877.
878.
H Reznik-Wolf TA Treves M Davidson J Aharon-Peretz PH St George Hyslop J Chapman AD Korczyn B Goldman E Friedman 《Canadian Metallurgical Quarterly》1996,98(6):700-702
Germline mutations in the presenilin 1 (PS1) gene apparently account for the majority of early-onset, familial Alzheimer's disease (AD). Using a mutation-screening strategy (denaturing gradient gel electrophoresis; DGGE), we analyzed a large family with early onset AD and seizures. The patients in this family showed a novel missense mutation in exon 5 of the PS1 gene (A to T change in codon 120, altering glutamine to aspartic acid). This novel mutation is located within the second hydrophilic domain of the molecule, a region not particularly involved in previously described germline mutations, and is of unknown biological significance. These results also demonstrate that DGGE can be used effectively to screen for mutations within this gene. 相似文献
879.
Abstract This study deals with the modeling of air pollution in apartments from laboratory measurements of source strengths, using formaldehyde and Total Volatile Organic Compounds (TVOCs) as model pollutants. The sources in two test apartments were grouped into two: building-related sources and occupant-related sources. The measured source strengths and ventilation rates were used for the prediction of concentrations expected in the apartments. These predictions were compared to measurements in the apartment over 12 months. The conclusions were that the model predictions based on emission rates measured in the laboratory can be used to predict the long-term concentration of the two model pollutants in the apartments. Considering the measured differences in ventilation between the apartments, an occupant emission rate of between 0.2 and 0.3 mg/h/kg body weight could be estimated. Based on previous suggested limits of acceptable exposures of humans to VOCs, an acceptable average emission rate of VOCs from building materials in general was estimated to be about 30 (μ/m2/h. The modeling showed that during the first 200 days, building materials dominated the emissions. After this, sources relating to the occupants dominated. On average about half of the VOC pollution originated from the building materials. 相似文献
880.
A new type of fiber laser modelocking is examined where a phase modulator is driven asynchronously with respect to a harmonic of the roundtrip cavity frequency. An asynchronous drive is able to produce high repetition rate (>1 GHz) soliton pulses that are shorter than the classic, active modelocking pulse width limit. It is shown that with a proper choice of fiber parameters, noise cleanup and soliton pulse shaping can be obtained using filtering and asynchronous phase modulation. Limits of asynchronous detuning are derived and a comparison with the parameters ranges for synchronous phase modulation is also presented 相似文献