System performance evaluation in terrestrial systems with highpolarization mode dispersion and the effect of chirping

We evaluate the system power penalty for different modulation formats-nonreturn-to-zero (NRZ), return-to-zero (RZ), dispersion-managed solitons, and prechirped RZ-in the presence of polarization mode dispersion (PMD) for 10-Gb/s terrestrial systems. All orders of PMD are considered by simulating the fiber using the coarse-step method, and a statistical approach is used to estimate the occasional fading of the signals. We show that pulses with lower duty-cycles perform better in general, and the system performance is improved if appropriate prechirping interacts with the residual chromatic dispersion of the fiber     

Enhancing the dynamic range and DGD monitoring windows in DOP-based DGD monitors using symmetric and asymmetric partial optical filtering

We propose and demonstrate a new type of degree-of-polarization (DOP)-based differential-group-delay (DGD) monitor using an optical filter such that the DGD monitoring range and DOP dynamic range are dramatically increased. We apply this technique to varying pulsewidth return-to-zero (RZ), carrier-suppressed RZ (CSRZ), and alternate-chirped RZ (ACRZ) signals and show that by optimally setting the position, bandwidth, and shape of a filter, we can double the DGD monitoring range compared to traditional DOP-based DGD monitors. Using our technique, the DGD monitoring ranges for 10, 20, and 40 Gb/s /spl sim/12.5-ps pulsewidth RZ signals are increased by 32, 33, and 12 ps, respectively. We also show that a narrow-band optical filter, offset from the center of the optical spectrum by the bit-rate frequency, can double the dynamic range of DOP-based DGD monitors for non-RZ (NRZ) signals.     

Gated Mesoporous SiO2 Nanoparticles Using K+‐Stabilized G‐Quadruplexes

The K⁺‐induced formation of G‐quadruplexes provides a versatile motif to lock or unlock substrates trapped in the pores of mesoporous SiO₂ nanoparticles, MP‐SiO₂ NPs. In one system, the substrate is locked in the MP‐SiO₂ NPs by K⁺‐ion‐stabilized G‐quadruplex units, and the pores are unlocked by the elimination of K⁺ ions using Kryptofix [2.2.2] (KP) or 18‐crown‐6‐ether (CE) from the G‐quadruplexes. In the second system, the substrate is locked in the pores by means of K⁺‐stabilized aptameric G‐quadruplex/thrombin units. Unlocking of the pores is triggered by the dissociation of the aptamer/thrombin complexes through the KP‐ or CE‐mediated elimination of the stabilizing K⁺ ions. In the third system, duplex DNA units lock the pores of MP‐SiO₂ NPs, and the release of the entrapped substrate is stimulated by the K⁺‐ion‐induced dissociation of the duplex caps through the formation of the K⁺‐stabilized G‐quadruplexes. The latter system is further implemented to release the anti‐cancer drug, doxorubicin, in the presence of K⁺ ions, from the MP‐SiO₂ NPs. Preliminary intracellular experiments reveal that doxorubicin‐loaded MP‐SiO₂ NPs lead to effective death of breast cancer cells.     

Bis-Aniline-Crosslinked Enzyme–Metal Nanoparticle Composites on Electrodes for Bioelectronic Applications

The electrical contacting of redox enzymes with electrodes is the most fundamental requirement for the development of amperometric biosensors and biofuel cell elements. We describe a novel method to prepare electrically contacted metallic nanoparticles (NPs) or carbon nanotubes (CNTs)/enzyme hybrid composites on electrodes that act as amperometric biosensors or as the constituents of biofuel cells. Au NPs or Pt NPs were modified with thioaniline electropolymerizable groups, and so were the enzymes glucose oxidase (GOx) or bilirubin oxidase (BOD). Electrochemical polymerization of the thioaniline-functionalized Pt NPs and GOx on a thioaniline monolayer-modified Au surface led to the formation of a bis-aniline-bridged Pt NPs/GOx composite electrode that enabled the analysis of glucose through the electrocatalyzed reduction of H₂O₂. Similarly, a Pt NPs/BOD composite-functionalized electrode showed electrocatalytic activity toward the reduction of O₂ to H₂O. Also, a Au NPs/GOx composite-functionalized electrode revealed direct electrical contacting between the enzyme and the electrode through the electrocatalytic reduction of the bis-aniline bridges, and this enabled the bioelectrocatalytic oxidation and the amperometric sensing of glucose. Finally, a biofuel cell consisting of an anode modified with Nile blue/NAD⁺/alcohol dehydrogenase on carbon nanotubes, and a cathode composed of the bis-aniline-crosslinked Pt NPs/BOD composite was constructed. The biofuel cell operates with a power output corresponding to 200 μW cm^-2.     

Free volume in PEP-silica nanocomposites with varying molecular weight

The influence of confinement in polymer-nanocomposites on free volume and glass transition of the polymer chains was studied. The molecular weight (Mw) of poly(ethylene-alt-propylene) (PEP) was varied from 3k to 200k and so the end-to end distance of the chains at fixed diameter (∼15 nm) and concentration (15%) of the silica nanoparticles increased. Thus the topological confinement increases with increasing Mw. Using hydrophobic PEP and particles functionalized with short organic molecules, we can rule out contributions of permanent adsorption of the chains. DSC showed no change in glass transition temperature. The decrease in the specific heat capacity could be explained by a simple mixing rule. By positron annihilation lifetime spectroscopy, taking properly into account contributions of the silica particles, we rule out an influence of the geometrical confinement on the free volume in the PEP nanocomposites studied here.     

Zeitverhalten der isothermen Reaktivdestillation beim thermischen Cracken und Desoxygenieren von Rapsöl

Thermal cracking of rapeseed oil under isothermal reactive distillation conditions allows the production of alternative liquid fuels. Temporal changes of the sump phase and the oil condensate show an increased higher heating value due to deoxygenation. The sump phase also shows an increasing thermal stability, accompanied by polymerization and aromatization. This is derived from a changing iodine value, H/C ratio as well as viscosity. The deoxygenation of the oil condensate is confirmed by a decreasing acid value and the reduction of detected carboxylic acids.     

Liposomes labeled with biotin and horseradish peroxidase: a probe for the enhanced amplification of antigen--antibody or oligonucleotide--DNA sensing processes by the precipitation of an insoluble product on electrodes

Liposomes labeled with biotin and the enzyme horseradish peroxidase (HRP) are used as a probe to amplify the sensing of antigen-antibody interactions or oligonucleotide-DNA binding. The HRP-biocatalyzed oxidation of 4-chloro-1-naphthol (1) in the presence of H2O2, and the precipitation of the insoluble product 2 on electrode supports, are used as an amplification route for the sensing processes. The anti-dinitrophenyl antibody (DNP-Ab) is sensed by a dinitrophenyl-L-cysteine antigen monolayer associated with an Au electrode. A biotinylated anti-IgG-antibody (Fc-specific) is linked to the antigen-DNP-Ab complex, and the biotin-labeled HRP-liposomes associate with the assembly through an avidin bridge. The biocatalyzed precipitation of 2 on the electrode increases the electron-transfer resistances at the electrode-solution interface or the electrode resistance itself. The binding events of the different proteins on the electrode and the biocatalyzed precipitation of 2 on the conductive support are followed by Faradaic impedance spectroscopy or constant-current chronopotentiometry. DNP-Ab concentrations as low as 1 x 10(-11) g x mL(-1) can be detected by this method. The labeled liposomes were also used for the amplified detection of DNA 3. The oligonucleotide 4, complementary to a part of the target DNA 3 that is a model nucleic acid sequence for the Tay-Sachs genetic disorder, is assembled on an Au electrode. Hybridization of the analyte 3 followed by the association of the biotin-tagged oligonucleotide 5 yields a three-component double-stranded assembly. Sensing of the analyte 3 is amplified by the association of avidin, the labeled liposomes, and the subsequent biocatalyzed precipitation of 2 on the electrodes. The DNA 3 is detected with a sensitivity that corresponds to 6.5 x 10(-13) M. Faradaic impedance spectroscopy and chronopotentiometry were employed to follow the stepwise assembly of the systems and the electronic transduction of the detection of the analyte DNA 3.     

Imprinting of nucleotide and monosaccharide recognition sites in acrylamidephenylboronic acid-acrylamide copolymer membranes associated with electronic transducers

Molecular recognition sites for the nucleotides adenosine 5'-monophosphate (1), guanosine 5'-monophosphate (2), cytosine 5'-monophosphate (3), and uridine 5'-monophosphate (4) are imprinted in an acrylamide-acrylamidephenylboronic acid copolymer (5) membrane. The imprinted membranes are assembled on piezoelectric Au quartz crystals or Au electrodes via electropolymerization or on the gate surface of an ISFET device by radical polymerization. The imprinted membranes reveal selectivity toward the imprinted nucleotide, and the association of the respective nucleotides with the recognition sites is transduced by the following: (i) microgravimetric, quartz crystal microbalance (QCM) measurements; (ii) Faradaic impedance analyses, and (iii) potentiometric responses of the ISFET devices. While the microgravimetric QCM measurements reflect the swelling of the polymers upon the association of the nucleotides with the recognition sites, the ISFET response is due to the charging of the polymer membrane as a result of the formation of the nucleotide-boronate complex. The selective detection of the nucleotides may lead to new DNA/RNA sequencing methods. Also, specific recognition sites for beta-D(+)-glucose (6), D(+)-galactose (7), and beta-D(-)-fructose (8) were imprinted in an acrylamide-acrylamidephenylboronic acid copolymer (5) membrane associated with an ISFET device. Selective sensing of the respective monosaccharides is accomplished in the presence of the imprinted membrane-functionalized ISFET devices.