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排序方式: 共有117条查询结果,搜索用时 171 毫秒
71.
Sarah S. Park Zachary J. Urbach Chase A. Brisbois Kelly A. Parker Benjamin E. Partridge Taegon Oh Vinayak P. Dravid Monica Olvera de la Cruz Chad A. Mirkin 《Advanced materials (Deerfield Beach, Fla.)》2020,32(4):1906626
Under an applied magnetic field, superparamagnetic Fe3O4 nanoparticles with complementary DNA strands assemble into crystalline, pseudo-1D elongated superlattice structures. The assembly process is driven through a combination of DNA hybridization and particle dipolar coupling, a property dependent on particle composition, size, and interparticle distance. The DNA controls interparticle distance and crystal symmetry, while the magnetic field leads to anisotropic crystal growth. Increasing the dipole interaction between particles by increasing particle size or external field strength leads to a preference for a particular crystal morphology (e.g., rhombic dodecahedra, stacked clusters, and smooth rods). Molecular dynamics simulations show that an understanding of both DNA hybridization energetic and magnetic interactions is required to predict the resulting crystal morphology. Taken together, the data show that applied magnetic fields with magnetic nanoparticles can be deliberately used to access nanostructures beyond what is possible with DNA hybridization alone. 相似文献
72.
Segura RA Ibáñez W Soto R Hevia S Häberle P 《Journal of nanoscience and nanotechnology》2006,6(7):1945-1953
Carbon nanotubes (CNTs) were synthesized by Chemical Vapor Deposition (CVD) from the pyrolytic decomposition of Iron Phthalocyanine (FePc) molecules, on SiO2/Si(111) substrates in the presence of a hydrogen flow. FePc molecules contribute simultaneously both to the formation of the precursor Fe nanoparticles and also as a Carbon source. Different experimental conditions were examined. Samples were characterized by scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and inverse photoemission. The resulting samples are highly oriented multiwall carbon nanotubes films, with heights in the range between: 4 and 20 microm. The tubes diameter is strongly dependent on growth temperature. Our experimental results show evidence of a transition in the growth mechanism, from a tip growth to a base growth mode, as the decomposition temperature is increased. Preliminary spectroscopic measurements performed on these MWCNTs, show the unoccupied density of states has several resonances close to Fermi level, related both to the graphene electronic structure and the formation of the tube. 相似文献
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Le Huérou JY Gindre M Amararene A Urbach W Waks M 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2003,50(11):1595-1600
We present a high precision ultrasonic velocimeter for a small volume sample (1 cm/sup 3/) for a path length of 1 cm achieved. The method used is based on the time of flight measurement with an original signal processing technique: the barycenter method. With our system, we have measured the sound velocity with an accuracy of 10/sup -5/. The detection of a difference in velocity between two liquids of about 2 cm/s is achieved. The compressibility of the reference liquid can then be deduced with an accuracy better than 0.2%. Using this custom-made system, we have studied and characterized complex fluids, systems biomimetic of biological membranes, as well as proteins included in nanometric water droplets. Under these experimental conditions, we have reached the value of protein compressibilities with an accuracy better than 10%. 相似文献
75.
Gudiksen KL Urbach AR Gitlin I Yang J Vazquez JA Costello CE Whitesides GM 《Analytical chemistry》2004,76(24):7151-7161
This paper uses capillary electrophoresis to follow a globular metalloprotein--bovine carbonic anhydrase II (BCA, EC 4.2.1.1)--on unfolding upon treatment with sodium dodecyl sulfate (SDS) and refolding upon removal of SDS, both in the presence and the absence of its Zn(II) cofactor. This research demonstrates that the Zn(II) cofactor is not required for refolding into a nativelike conformation, does not remain associated with the unfolded protein, and does not significantly change the rate of refolding. The presence of the Zn(II) cofactor, however, does increase the total amount of recovered protein by a factor of 2. Capillary electrophoresis could distinguish between native and denatured protein, based on the difference in electrophoretic mobility between the native protein and the aggregate of denatured protein and SDS. In addition, the active site was probed by observing binding of BCA to a charged arylsulfonamide using affinity capillary electrophoresis. These studies provide a foundation for future physical-organic studies using BCA as a model to examine interactions between proteins and SDS. 相似文献
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Oleh Smutok Maria Karkovska Tetiana Prokopiv Taras Kavetskyy Wladimir Sibirnyj Mykhailo Gonchar 《Yeast (Chichester, England)》2019,36(5):341-348
During the recent decades, a lot of data about the significance of D-lactate determination in food technology and quality control have been accumulated. Nowadays, the development of new methods for the determination of D-lactate is very relevant, especially with regard to biosensors. To construct a D-lactate-selective biosensor, we suggest using the mitochondria of recombinant yeast cells of Ogataea (Hansenula) polymorpha “tr6” (gcr1 catX/Δcyb2, prAOX_DLDH) overproducing D-lactate: cytochrome c-oxidoreductase (DLDH, EC 1.1.2.4) and lacking an L-lactate-specific enzyme (flavocytochrome b2, E.C. 1.1.2.3). The usage of the pure enzyme is problematic due to the complexity of its isolation and stabilization because of the intramembranous localization of DLDH. The enzyme catalyzes D-lactate oxidation to pyruvate coupled with ferricytochrome c reduction to ferrocytochrome c. The constructed biosensor is characterized by high sensitivity (18.5 А·М−1·m−2), a low detection limit (3 μM of D-lactate), wide linear ranges, good selectivity, and sufficient stability. The real samples' analysis of D-lactate in dairy products was performed, and high correlation of the obtained results with the reference approach (0.7 < r < 1) and literature data was demonstrated. 相似文献
79.
Information Systems and e-Business Management - In our research, we suggest a process theory for explaining the strategy assessment process and its effect in information systems (IS) planning. The... 相似文献