D-lactate-selective amperometric biosensor based on the mitochondrial fraction of Ogataea polymorpha recombinant cells

During the recent decades, a lot of data about the significance of D-lactate determination in food technology and quality control have been accumulated. Nowadays, the development of new methods for the determination of D-lactate is very relevant, especially with regard to biosensors. To construct a D-lactate-selective biosensor, we suggest using the mitochondria of recombinant yeast cells of Ogataea (Hansenula) polymorpha “tr6” (gcr1 catX/Δcyb2, prAOX_DLDH) overproducing D-lactate: cytochrome c-oxidoreductase (DLDH, EC 1.1.2.4) and lacking an L-lactate-specific enzyme (flavocytochrome b₂, E.C. 1.1.2.3). The usage of the pure enzyme is problematic due to the complexity of its isolation and stabilization because of the intramembranous localization of DLDH. The enzyme catalyzes D-lactate oxidation to pyruvate coupled with ferricytochrome c reduction to ferrocytochrome c. The constructed biosensor is characterized by high sensitivity (18.5 А·М⁻¹·m⁻²), a low detection limit (3 μM of D-lactate), wide linear ranges, good selectivity, and sufficient stability. The real samples' analysis of D-lactate in dairy products was performed, and high correlation of the obtained results with the reference approach (0.7 < r < 1) and literature data was demonstrated.     

Application of active queue management for real-time adaptive video streaming

Telecommunication Systems - Video streaming currently dominates global Internet traffic. Live streaming broadcasts events in real-time, with very different characteristics compared to...     

A novel, single-mode piezoceramic plate actuator for ultrasonic linear motors

A new type of piezoelectric plate actuator for ultrasonic linear motors has been developed. These new piezoelectric actuators use the principle of asymmetric resonant excitation of the piezoceramic plate in a special resonant mode consisting of a standing two-dimensional extensional wave in a piezoceramic plate. The behavior of the actuator has been simulated with finite-element method (FEM) software and the simulation results checked with single-point contact measurements on the surface of the actuator. This paper describes this work and closes by describing the new ultrasonic translation stages based on this design.     

Paschen testing of ITER prototype cryogenic axial breaks

Axial insulation breaks are needed in forced cooled cryogenic high voltage devices for the separation of the high voltage area from the grounded pipe system. The ITER cryogenic axial breaks will be surrounded by good vacuum in case of normal operation but also under vacuum breakdown conditions sufficient dielectric strength is required for a reliable fast discharge of the coil system. A Paschen tight design of the ITER prototype breaks enables high voltage operation independent on the outer vacuum or gas conditions. Consecutively two pretested ITER prototype breaks were integrated in the insulation system of a Paschen test unit and high voltage tested. Two different ways to perform the Paschen testing were used for both breaks. The preparation of the breaks and the test setup are described and the test results are given.     

Rigorous model of the scattering of a focused spot by a grating and its application in optical recording

We describe a rigorous model for the scattering of a three-dimensional focused spot by a one-dimensional periodic grating. The incident field is decomposed into a sum of quasi-periodic fields, and the scattering of each of these is computed inside one unit cell of the grating. The model is applied to the simulation of the readout of a DVD disk. The polarization dependence of the reflected near and far fields is studied, and, for a TM-polarized incident spot, plasmons are observed in the reflected far-field intensity.     

Mumford–Shah Model for One-to-One Edge Matching

This paper presents a new algorithm based on the Mumford-Shah model for simultaneously detecting the edge features of two images and jointly estimating a consistent set of transformations to match them. Compared to the current asymmetric methods in the literature, this fully symmetric method allows one to determine one-to-one correspondences between the edge features of two images. The entire variational model is realized in a multiscale framework of the finite element approximation. The optimization process is guided by an estimation minimization-type algorithm and an adaptive generalized gradient flow to guarantee a fast and smooth relaxation. The algorithm is tested on T1 and T2 magnetic resonance image data to study the parameter setting. We also present promising results of four applications of the proposed algorithm: interobject monomodal registration, retinal image registration, matching digital photographs of neurosurgery with its volume data, and motion estimation for frame interpolation.     

Effektives Steuern von IT-Outsourcingdienstleistern

IT executives entering into information technology (IT) outsourcing arrangements seek various strategic, economic, and technological benefits. However, although several cases of IT outsourcing are considered successful, cases of failure can also be observed. Problems and challenges associated with IT outsourcing often not only relate to the strategic decision whether or not to outsource, but to the operational level as well. Especially organizations with little experience of implementing larger IT outsourcing programs face problems with the steering of external outsourcing providers. In this paper, we propose a reference framework that structures the required processes for an effective steering of IT outsourcing relationships. The research is based on the design science paradigm in information systems research. In a first step, we derive a framework from related literature and knowledge in this particular area. We then undertake extensive fieldwork, including expert interviews and field studies to evaluate our framework and to develop it further. The suggested framework proves to be a viable instrument to support the systematic analysis of current processes and the definition of suitable target processes for the steering of IT outsourcing programs. This paper??s primary contribution therefore lies in providing an applicable instrument for practitioners as well as in extending the existing body of knowledge on IT outsourcing governance.     

CGTase‐Catalysed cis‐Glucosylation of L‐Rhamnosides for the Preparation of Shigella flexneri 2a and 3a Haptens

We report the enzymatic synthesis of α‐D ‐glucopyranosyl‐(1→4)‐α‐L ‐rhamnopyranoside and α‐D ‐glucopyranosyl‐(1→3)‐α‐L ‐rhamnopyranoside by using a wild‐type transglucosidase in combination with glucoamylase and glucose oxidase. It was shown that Bacillus circulans 251 cyclodextrin glucanotransferase (CGTase, EC 2.1.4.19) can efficiently couple an α‐L ‐rhamnosyl acceptor to a maltodextrin molecule with an α‐(1→4) linkage, albeit in mixture with the α‐(1→3) regioisomer, thus giving two glucosylated acceptors in a single reaction. Optimisation of the CGTase coupling reaction with β‐cyclodextrin as the donor substrate and methyl or allyl α‐L ‐rhamnopyranoside as acceptors resulted in good conversion yields (42–70 %) with adjustable glycosylation regioselectivity. Moreover, the efficient chemical conversion of the products of CGTase‐mediated cis‐glucosylation into protected building blocks (previously used in the synthesis of O‐antigen fragments of several Shigella flexneri serotypes) was substantiated. These novel chemoenzymatic strategies towards useful, convenient intermediates in the synthesis of S. flexneri serotypes 2a and 3a oligosaccharides might find applications in developments towards synthetic carbohydrate‐based vaccine candidates against bacillary dysentery.