Molecular cloning and nucleotide sequencing of a novel aminoglycoside 6'-N-acetyltransferase gene from an R-plasmid of Salmonella typhimurium S24 isolated in Taiwan

A conjugative aminoglycoside resistance plasmid pST2 has been isolated from Escherichia coli K-12 14R525, which was mated with a clinical isolate of Salmonella typhimurium S24. A novel resistance gene of aminoglycoside 6'-N-acetyltransferase[AAC(6')] was cloned from plasmid pST2 on a 1,393 kilobase (kb) of SphI-SalI fragment into vector pACYC184 and pUC18. This novel AAC(6') gene in plasmid pST2 acetylated kanamycin, amikacin, dibekacin, tobramycin, gentamicin, netilmicin, and sisomicin. The complete nucleotide sequence of the novel AAC(6') gene and its neighboring sequences were also determined. Minicell experiments detected only one protein of 24.7 kilodaltons (kDa) translated from an open reading frame of the 618 base pairs (bp) gene.     

A theoretical study of pulsed laser sampling photon amplifiers

Theoretical studies point to significant improvements in the performance of semiconductor laser amplifiers by injecting carriers with pulsed electric currents of sub-nanosecond duration. A pulsed Fabry-Perot amplifier (FPA) is most sensitive to input lightwave at the instant the carrier density is crossing the critical region, and gives a sharply pulsed sampling effect on the input lightwave signal. Compared with a FPA operating at subcritical electron density, the pulsed amplifier gives much higher gain, peak power, and bandwidth. In fact, pulsed operation of a FPA is also expected to give significantly higher gain and about the same peak output power as a traveling wave amplifier. Pulsed operation also improves the performance of a traveling wave amplifier by attenuating its internally reflected waves     

Effects of free convection and mass transfer on the flow past an impulsively moving infinite vertical circular cylinder

The effects of free convection and mass transfer are taken into account for the Stokes' problem of the flow near an impulsively moving infinite vertical circular cylinder. Expressions of the velocity, temperature, concentration and skin friction of the fluid in closed form are obtained by the Laplace transform technique. The results based on various values of the parameters Gr (Grashof number), Gm (modified Grashof number), Sc (Schmidt number) and Pr (Prandtl number) are given in graphical form. It will be seen that there is a rise in the velocity due to the presence of a foreign mass. But higher Sc yields the lower velocity and skin friction. As the radius of the circular cylinder approaches to infinite, the results presented in this paper agree with those of V.M. Soundalgekar's and C.K. Chen's etc. for the flow past an impulsively moving infinite vertical plate.     

An experimental investigation of electrically thick rectangular microstrip antennas

The electromagnetic properties of electrically thick rectangular microstrip antennas were investigated experimentally. Antennas were fabricated with different patch sizes and with electrical thicknesses ranging from 0.03 to 0.23 wavelengths in the dielectric substrate. The resonant frequencies were measured and compared to existing formulas. The bandwidth was calculated as a function of electrical thickness and the antenna radiation patterns were measured.     

Noninterfering optical method of HBT circuit evaluation

The authors report a novel noninterfering and simple approach for evaluation of circuits implemented with AlGaAs/GaAs heterojunction bipolar transistors (HBTs). This method makes use of radiative recombination in the base region of current-carrying HBTs. The infrared radiation emitted is 'visible' to the closed-circuit TV (CCTV) cameras. Therefore, one can view the operation of the HBT circuit under test at normal biases with a TV monitor. This method can be used to determine logic states of gates, as well as collector current of individual HBTs within integrated circuits.<>     

Polarization independent isolator using spatial walkoff polarizers

A novel type of polarization-independent optical isolator is described which utilizes the polarization walkoff provided by birefringent crystals. Isolation of 44 dB is measured at a wavelength of 1.3 mu m with single-mode fiber insertion loss of 1.5 dB. A temperature-independent scheme is also described that provides at least 39 dB of isolation over the temperature range of 25-75 degrees C.<>     

High performance liquid chromatographic separation of stereoisomeric bile acids as their UV-sensitive esters

High performance liquid chromatographic separation of a series of mono-, di- and trihydroxylated 5β-cholanic acids which differ only in position and configuration of hydroxyl groups at positions C-3, C-7 and/or C-12, is reported. The C-24 free acids were derivatized to four different classes of UV-sensitive esters, i.e.,p-bromophenacyl (BP),m-methoxyphenacyl (MP), 4-nitrophthalimidemethyl (NPM) and 9-anthrylmethyl (AM) esters, and chromatographed on two, variants of C₁₈ reversed-phase columns (Nova-Pak C₁₈ and Zorbax ODS) with methanol-water systems as mobile phase. Separation efficiency and elution order of some isomeric pairs were influenced by both the structure of the C-24 ester groups and the nature of the columns used. Excellent chromatographic properties were found for those derivatives, particularly for the NPM esters.     

China Likely to Resume Oil Futures Soon

China is likely to resume operation of its oil futures this year, according to reports from the media about a futures conference organized by the Shanghai Futures Exchange (SHFE) in late May. "As China has become an important oil producer and consumer,the demand for our own oil futures market emerges,which will help China oil-related enterprises hedge risks," said Li Ruisheng, vice president of PetroChina's refining and marketing company.