Crosstalk between Growth and Osmoregulation of GHRH-SST-GH-IGF Axis in Triploid Rainbow Trout (Oncorhynchus mykiss)

Smolting is an important development stage of salmonid, and an energy trade-off occurs between osmotic regulation and growth during smolting in rainbow trout (Oncorhynchus mykiss). Growth hormone releasing hormone, somatostatin, growth hormone and insulin-like growth factor (GHRH-SST-GH-IGF) axis exhibit pleiotropic effects in regulating growth and osmotic adaptation. Due to salmonid specific genome duplication, increased paralogs are identified in the ghrh-sst-gh-igf axis, however, their physiology in modulating osmoregulation has yet to be investigated. In this study, seven sst genes (sst1a, sst1b, sst2, sst3a, sst3b, sst5, sst6) were identified in trout. We further investigated the ghrh-sst-gh-igf axis of diploid and triploid trout in response to seawater challenge. Kidney sst (sst1b, sst2, sst5) and sstr (sstr1b1, sstr5a, sstr5b) expressions were changed (more than 2-fold increase (except for sstr5a with 1.99-fold increase) or less than 0.5-fold decrease) due to osmoregulation, suggesting a pleiotropic physiology of SSTs in modulating growth and smoltification. Triploid trout showed significantly down-regulated brain sstr1b1 and igfbp2a1 (p < 0.05), while diploid trout showed up-regulated brain igfbp1a1 (~2.61-fold, p = 0.057) and igfbp2a subtypes (~1.38-fold, p < 0.05), suggesting triploid trout exhibited a better acclimation to the seawater environment. The triploid trout showed up-regulated kidney igfbp5a subtypes (~6.62 and 7.25-fold, p = 0.099 and 0.078) and significantly down-regulated igfbp5b2 (~0.37-fold, p < 0.05), showing a conserved physiology of teleost IGFBP5a in regulating osmoregulation. The IGFBP6 subtypes are involved in energy and nutritional regulation. Distinctive igfbp6 subtypes patterns (p < 0.05) potentially indicated trout triggered energy redistribution in brain and kidney during osmoregulatory regulation. In conclusion, we showed that the GHRH-SST-GH-IGF axis exhibited pleiotropic effects in regulating growth and osmoregulatory regulation during trout smolting, which might provide new insights into seawater aquaculture of salmonid species.     

Early Expression of Tet1 and Tet2 in Mouse Zygotes Altered DNA Methylation Status and Affected Embryonic Development

Ten-eleven translocation (Tet) dioxygenases can induce DNA demethylation by catalyzing 5-methylcytosine(5mC) to 5-hydroxymethylcytosine(5hmC), and play important roles during mammalian development. In mouse, Tet1 and Tet2 are not expressed in pronucleus-staged embryos and are not involved in the genomic demethylation of early zygotes. Here, we investigated the influence of Tet1 and Tet2 on methylation of parental genomes by ectopically expressing Tet1 and Tet2 in zygotes. Immunofluorescence staining showed a marked 5hmC increase in the maternal pronucleus after injection of Tet1 or Tet2 mRNA into zygotes. Whole-genome bisulfite sequencing further revealed that Tet2 greatly enhanced the global demethylation of both parental genomes, while Tet1 only promoted the paternal demethylation. Tet1 and Tet2 overexpression altered the DNA methylation across genomes, including various genic elements and germline-specific differently methylated regions. Tet2 exhibited overall stronger demethylation activity than Tet1. Either Tet1 or Tet2 overexpression impaired preimplantation embryonic development. These results demonstrated that early expression of Tet1 and Tet2 could substantially alter the zygotic methylation landscape and damage embryonic development. These findings provide new insights into understanding the function of Tet dioxygenases and the mechanism of DNA methylation in relation to embryogenesis.     

多主体证书收集系统模型及优化方法

以支持信任链的快速重建为对象,研究面向多移动主体的信任证书收集模型及其优化问题。首先给出了实体、证书以及角色关系的形式化定义和级联信任的拓扑结构,随后详细阐述了基于开放服务网关规范中间件技术的证书收集系统软件模型,其核心为可在网络中跨域迁移的主体组件,并针对多主体协作过程中影响系统性能发挥的两类动态行为：寻径与繁殖,分别提出了基于改进旅行商问题的求解方法和两种方式繁殖原理的优化策略。最后通过Monte-Carlo仿真实验证明了模型和优化方法的有效性。     

相变换热器应用于电站锅炉的探讨

阐述了复合相变换热器是一种新型的换热器,可以控制低温受热面金属不发生低温腐蚀,降低烟气的排放温度,该技术可用于各种燃煤、燃油、燃气锅炉、窑炉、注气炉、加热炉等换热设备,目前复合相变换热器应用于工业锅炉和循环流化床已有不少成功案例[1-3],而应用于大型电站锅炉还很少有报道,结合某330 MW电站锅炉尾部加装复合相变换热器的运行情况,对复合相变换热器应用于电站锅炉的运行情况及优缺点做了简要探讨。     

无线频谱测量方法与分析

文章基于无线频谱测量的方法和要素,从各个角度分析了现有的频谱占用情况,测量结果表明,现有的频率分配政策使得部分频谱并未被有效地利用,频谱占用度在时间上空间上具有较大的差异性.测量结果将为未来的认知无线电技术等下一代无线通信技术的实现提供依据.     

Low-latency and low-overhead encoder ASIC in the ATLAS LAr calorimeter trigger upgrade

In this article, an encoder Application Specific Integrated Circuit (ASIC) for high-speed serial data transmission is presented. The ASIC implements a low-latency and low-overhead line code and is fabricated with a commercial 0.25-µm Silicon-on-Sapphire CMOS technology. The ASIC operates at 640 MHz with a latency of no greater than 6.25 ns and the overhead of 14.3%. The encoder will be integrated with a serialiser and will be used in the A Toroidal LHC ApparatuS Liquid Argon (LAr) calorimeter Phase-I trigger upgrade.     

Fermi-Level Pinning in Nanocrystal Memories

The nanocrystal (NC) work-function engineering, which plays an important role on the NC memory characteristics such as memory window and retention time, were long regarded as a matter of choice on NC materials. In this letter, we report opposite polarities of charge storage in Au NC memories with different control oxides. The effective NC work function is found to be not only a bulk property of the NC, but also governed by the interface with surrounding dielectric, as a result of the Fermi-level pinning. By replacing Au NCs with C₆₀ molecules, we also show the pinning effect generally exists at quantum-dot-based devices with high density of interface states. This fundamental interface property should be taken into account in the selection of NC and dielectric materials for the NC memory optimization     

In situ synchrotron X-ray diffraction analysis of deformation behavior of a Nb/NiTi composite for biomedical applications

The deformation behavior involved in Nb/NiTi composite for biomedical applications within a large macroscopic strain range was investigated by tensile loading-unloading test and in situ synchrotron X-ray diffraction(SXRD).Experimental results show that during loading,the Nb/NiTi composite experiences the elastic elongation of B2-NiTi austenitic,B19'-NiTi martensitic and β-Nb phases,B2→ B19' stress-induced martensitic(SIM) transformation and tensile plastic deformation of β-Nb phase.During unloading,the deformation behavior involved in Nb/NiTi composite includes the elastic recovery of B2-NiTi austenitic,B19'-NiTi martensitic and β-Nb phases,reverse phase transformation B19'→B2 and compressive deformation of p-Nb phase.The martensitic transformation in this composite is almost reversible and occurs in a localized manner.These results might contribute to a comprehensive understanding of the deformation mechanism involved in Nb/NiTi composite and shed some light on design and development of novel composites with a combination of good biocompatibility and excellent superelasticity for biomedical applications.     

Sogatella furcifera Saliva Mucin-like Protein Is Required for Feeding and Induces Rice Defences

The white-backed planthopper (WBPH), Sogatella furcifera, is one of the most important piercing-sucking pests of rice (Oryza sativa) in Asia. Mucin-like salivary protein (SFMLP) is highly expressed in the salivary glands of WBPH, which plays an important role in WBPH feeding. In this study, WBPH injected with dsSFMLP had difficulty in sucking phloem sap from rice plants, which significantly reduced their food intake, weight, and survival. In contrast, the knockdown of the SFMLP gene had only a marginal effect on the survival of WBPH fed an artificial diet. Further studies showed that silencing SFMLP resulted in the short and single-branched salivary sheaths secretion and less formation of salivary flanges in rice. These data suggest that SFMLP is involved in the formation of the salivary sheath and is essential for feeding in WBPH. Overexpression of the SFMLP gene in rice plants promoted the feeding of WBPH, whereas silencing the gene in rice plants significantly decreased WBPH performance. Additionally, it was found that overexpression of SFMLP in rice plants elicited the signalling pathway of SA (salicylic acid) while suppressing JA (jasmonic acid); in contrast, silencing of the SFMLP gene in rice plants showed the opposite results. This study clarified the function of SFMLP in WBPH feeding as well as mediating rice defences.     

叶绿素荧光量子产量的MODIS遥感算法

从荧光量子产量的原理出发,利用 MODIS 卫星数据建立了一种估算量子产量的算法--φ算法.通过 2006 年 3 月 23 日渤海和北黄海的 MODIS 数据计算了荧光量子产量,并与叶绿素荧光效率(cFE)产品进行了比较.结果表明,φ算法是一种更精确的荧光量子产量的估算算法,它有效地避免了 CFE 存在的几个误差来源:包括未考虑浮游植物的"包裹效应"、使用 412nm 的离水辐亮度反演的浮游植物吸收系数以及积分深度过浅.CFE在大部分海域出现负值,还出现了某些高估点;在有效数据范围内,两者存在明显的线性相关关系.φ算法估算的量子产量最高值在0.05左右,这与其它海区测量的值域范围基本一致.除辽东湾东北部和部分近岸水域外,大部分离岸水域在0.001～0.02的范围内.通过对φ算法的误差来源分析,认为发展一种新的反演 678nm 比吸收系数和平均吸收系数的波段比值算法是可行的,也是改进φ算法精度的有效方法.