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Yao Yu Lu Ma Xinying Wang Zhi Zhao Wei Wang Yunxin Fan Kunquan Liu Tingting Jiang Ziwei Xiong Qisheng Song Changqing Li Panting Wang Wenjing Ma Huanan Xu Xinyu Wang Zijing Zhao Jianfei Wang Hongsheng Zhang Yongmei Bao 《International journal of molecular sciences》2022,23(10)
Rice blast is one of the main diseases in rice and can occur in different rice growth stages. Due to the complicated procedure of panicle blast identification and instability of panicle blast infection influenced by the environment, most cloned rice resistance genes are associated with leaf blast. In this study, a rice panicle blast resistance gene, Pb2, was identified by genome-wide association mapping based on the panicle blast resistance phenotypes of 230 Rice Diversity Panel 1 (RDP1) accessions with 700,000 single-nucleotide polymorphism (SNP) markers. A genome-wide association study identified 18 panicle blast resistance loci (PBRL) within two years, including 9 reported loci and 2 repeated loci (PBRL2 and PBRL13, PBRL10 and PBRL18). Among them, the repeated locus (PBRL10 and PBRL18) was located in chromosome 11. By haplotype and expression analysis, one of the Nucleotide-binding domain and Leucine-rich Repeat (NLR) Pb2 genes was highly conserved in multiple resistant rice cultivars, and its expression was significantly upregulated after rice blast infection. Pb2 encodes a typical NBS-LRR protein with NB-ARC domain and LRR domain. Compared with wild type plants, the transgenic rice of Pb2 showed enhanced resistance to panicle and leaf blast with reduced lesion number. Subcellular localization of Pb2 showed that it is located on plasma membrane, and GUS tissue-staining observation found that Pb2 is highly expressed in grains, leaf tips and stem nodes. The Pb2 transgenic plants showed no difference in agronomic traits with wild type plants. It indicated that Pb2 could be useful for breeding of rice blast resistance. 相似文献
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Lu-Yu Yan Jia-Gui Guo Xin Zhang Yang Liu Xin-Xin Xiong Yu-Xuan Han Li-Li Zhang Xiao-Hong Zhang Dong-Hong Min 《International journal of molecular sciences》2022,23(13)
The peptidase M24 (Metallopeptidase 24, M24) superfamily is essential for plant growth, stress response, and pathogen defense. At present, there are few systematic reports on the identification and classification of members of the peptidase M24 proteins superfamily in wheat. In this work, we identified 53 putative candidate TaM24 genes. According to the protein sequences characteristics, these members can be roughly divided into three subfamilies: I, II, III. Most TaM24 genes are complex with multiple exons, and the motifs are relatively conserved in each sub-group. Through chromosome mapping analysis, we found that the 53 genes were unevenly distributed on 19 wheat chromosomes (except 3A and 3D), of which 68% were in triads. Analysis of gene duplication events showed that 62% of TaM24 genes in wheat came from fragment duplication events, and there were no tandem duplication events to amplify genes. Analysis of the promoter sequences of TaM24 genes revealed that cis-acting elements were rich in response elements to drought, osmotic stress, ABA, and MeJA. We also studied the expression of TaM24 in wheat tissues at developmental stages and abiotic stress. Then we selected TaM24-9 as the target for further analysis. The results showed that TaM24-9 genes strengthened the drought and salt tolerance of plants. Overall, our analysis showed that members of the peptidase M24 genes may participate in the abiotic stress response and provided potential gene resources for improving wheat resistance. 相似文献
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集成膜工艺海水淡化与浓海水综合利用 总被引:1,自引:0,他引:1
综述了反渗透一电渗析集成工艺海水淡化与浓缩水综合利用研发现状与发展趋势。指出具有特殊分离功能的离子交换膜电渗析技术是高度分离、富集海洋化学元素的关键技术。提出了目前需要研究的几个问题。 相似文献
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Zicheng Li Yifeng Gao Zhihao Zhang Qiu Xiong Longhui Deng Xiaochun Li Qin Zhou Yuanxing Fang Peng Gao 《纳微快报(英文)》2021,(7):12-27
Efficient electron transport layers(ETLs)not only play a crucial role in promoting carrier separation and electron extraction in perovskite solar cells(PSCs)but... 相似文献