Optimisation of enzymatic hydrolysis for concentration of squalene in palm fatty acid distillate

BACKGROUND: Squalene was concentrated from palm fatty acid distillate (PFAD) in this study using commercial immobilised Candida antarctica lipase (Novozyme 435^®). The PFAD was neutralised (NPFAD) using an alkali to liberate the free fatty acids and then hydrolysed at 65 ± 1 °C. The enzymatic hydrolysis on NPFAD was optimised using response surface methodology (RSM) before being neutralised again to obtain a concentrated squalene fraction. RESULTS: A five‐level, three‐factor central composite rotatable design was adopted to evaluate the effects of the enzymatic hydrolysis parameters reaction time (4–12 h), water content (50–70% w/w) and enzyme concentration (1.5–3.5% w/w) on the percentage yield of squalene concentration. The optimal reaction parameters for maximum yield of squalene concentration were identified from the respective contour plots. The optimal enzymatic hydrolysis conditions were a reaction time of 7.05 h, a water content of 61.40% w/w and an enzyme concentration of 2.23% w/w. CONCLUSION: RSM was used to determine the optimal conditions for enzymatic hydrolysis of NPFAD with C. antarctica lipase for maximum recovery of squalene which could be implemented on an industrial scale. Copyright © 2008 Society of Chemical Industry     

Inhibitory effects of Hibiscus sabdariffa L extract on low‐density lipoprotein oxidation and anti‐hyperlipidemia in fructose‐fed and cholesterol‐fed rats

Hibiscus sabdariffa L extract (HSE) is an aqueous extract of Hibiscus sabdariffa L flowers that is used as a local soft drink and medical herb in Taiwan. Oxidation of low‐density lipoprotein (LDL) has been shown to increase the incidence of atherosclerosis. In this study, we determined the antioxidative activity of HSE on LDL oxidation by examining relative electrophoretic mobilities (REM) and thiobarbituric acid‐reactive substances (TBARS). The data revealed an inhibitory effect of HSE on Cu²⁺‐mediated REM and TBARS. HSE exhibited a remarkable ability to reduce cholesterol degradation and ApoB fragmentation. Overall, HSE showed a high potency to inhibit the production of oxidized LDL induced by copper and, specifically, to reduce serum triglycerides in high‐fructose diet (HFD) fed rats and serum cholesterol in high‐cholesterol diet (HCD) fed animals. The levels of LDL and the ratio of LDL‐cholesterol (LDL‐C) to HDL‐cholesterol (HDL‐C) were reduced by HSE in both hyperlipidaemia models. Based on these findings, we suggest that HSE may be used to inhibit LDL oxidation and to prevent various types of hyperlipidaemia in HFD‐ or HCD‐fed rats. Copyright © 2004 Society of Chemical Industry     

SGIP扩展协议对通信层计费的处理

对SGIP扩展协议中的通信层计费进行处理,提供相应的指令、功能、要求。介绍了用户鉴权的定义．以及浆权的步骤。讲述3种不同的计费方式,并对计费进行分类,给出相关的字符。论述了消息在SMSC和SP之间传输时的路由选择。     

多层次结构与半监督学习的谣言检测研究

当前谣言检测工作主要基于监督学习,需要人为标记数据而导致检测具有滞后性。为了充分利用大量的未标记数据,及时检测社交网络中的虚假谣言。提出了一种基于多层次结构与半监督学习谣言检测模型(multi-level semi spuervised graph convolutional neural network, MSGCN)。该模型构建了一种多层次检测模块,基于图卷积网络对有限的标记样本进行训练以提取多层次传播结构特征、扩散结构特征和全局结构特征。其次,引入随机模型扰动集成无标签数据的动态输出进行一致性预测,提出互补伪标签法来获取高质量伪标签数据,并将其加入标记数据扩充样本。最后在有监督交叉熵损失和无监督一致性损失约束下提高模型质量。在公开的Twitter15、Twitter16和Weibo数据集上的实验结果表明,所提出模型在30%标记样本下准确率达到88.3%、90.1%和95.5%,在少量的标记样本下便可达到优异的成绩。     

界面约束作用下Ti/Al多层复合材料的力学性能和冲压成形性能

采用热压+热轧复合法在500 ℃制备了3层、5层和7层配置的Ti/Al多层复合材料（LMCs）,研究了拉伸和埃里克森杯突试验过程中复合板的裂纹萌生和扩展行为,分析了界面约束效应对复合板力学性能和冲压成形性能的影响机理。结果表明,复合板界面具有微米级厚度的金属间相,导致其具有较强的界面结合。随复合板层数的增加,其屈服强度（YS）和极限抗拉强度（UTS）增加,延伸率（EL）和韧性降低,且由于热轧形成了强基面织构,复合板力学性能的各向异性明显增加。同时,复合板的加工硬化指数（n）和塑性应变比（r）均降低,屈强比（σ_s/σ_b）增大,这些均导致了复合板的冲压成形性能变差。对于层数较少的复合板,在断裂过程中界面脱粘起了主要作用。由于界面结合不佳,界面易发生分层,通过抑制裂纹萌生、促进裂纹偏转和钝化、降低裂纹扩展的驱动力,有效地延缓了复合板的断裂失效。     

前馈式智能控制隧道通风系统

通过对前馈式通风控制3种方式的比选,简述了长大隧道采用前馈式智能通风控制的优点,建立了该系统的交通流基本参数和风压模型以及工程设计原则,通过工程实施隧道污染物控制在设定值范围。     

Combined effects of Diospyros lotus leaf and grape stalk extract in high-fat-diet-induced obesity in mice

Food Science and Biotechnology - The aim of this study was to investigate the combined effects of Diospyros lotus leaves extracts (DLE) and Muscat Bailey A grape stalk extracts (MGSE) in obesity...     

Hot Compression Mechanical Behavior of Solution Heat-Treated and Pre-aged Mg-Zn-Gd-Er Alloys

The mechanical behavior of solution heat-treated and pre-aged Mg-6Zn-1Gd-1Er alloys during hot compression (from 180 to 330 °C) has been investigated. The results showed that the flow stress curves of the pre-aged sample (PAS) intersected with that of the solution heat-treated sample (SHTS) during hot compression. At 180 °C, when the true strain is 0.27 and 0.47, the PAS showed larger and smaller stress (210.80 MPa vs. 207.58 MPa and 205.67 MPa vs. 207.93 MPa) than the SHTS, respectively. These phenomena were due to the stronger interaction of W phase and dislocations/twins under the strain of 0.27, while dynamic recrystallization softening occurred under the strain of 0.47. When the temperature increased to 330 °C, the flow stress of PAS and SHTS showed an opposite trend to that of 180 °C. Continuous dynamic recrystallization and particle stimulated nucleation based on slip operations are the main deformation mechanisms under 330 °C. At the true strain is 0.33 and 0.53, the PAS has smaller and larger stress (61.32 MPa vs. 63.69 MPa and 58.75 MPa vs. 57.09 MPa) than the SHTS, respectively. The increasing deformation resistance of dynamic precipitation improved the flow stress under smaller strain and dynamic recrystallization decreased the flow stress under high strain, which resulted the opposite phenomena of SHTS.     

Determination of anisidine value in thermally oxidized palm olein by fourier transform infrared spectroscopy

Fourier transform infrared (FTIR) spectroscopy with transmission cell is described to predict anisidine value of palm olein. The calibration set was prepared by mixing the thermally oxidized palm olein and the unoxidized palm olein with certain ratios (w/w) covering a wide range of anisidine values. A partial least square (PLS) regression technique was employed to construct a calibration model. This model was further accomplished by a validation step. The standard error of prediction found was 0.51. The precision of this method was shown to be comparable to the accuracy of the American Oil Chemists’ Society method used for measurement of anisidine value, with coefficient of determination (R ²) of 0.99. The study showed that mid-band FTIR spectroscopy combined with a PLS calibration technique is a versatile, efficient, and accurate technique for the estimation of anisidine value of palm olein within about 2 min with less than 2 mL of sample.