On the inclusion of externally controlled actions in action planning.

According to ideomotor theories, perceiving action effects produced by others triggers corresponding action representations in the observer. We tested whether this principle extends to actions performed by externally controlled limbs and tools. Participants performed a go–no-go version of a spatial compatibility task in which their own actions resulted in the movement of a limb or tool, whereas a second, externally controlled limb or tool was seen performing a complementary go–no-go task. Spatial compatibility effects, indicating inclusion of the externally controlled effector in action planning, were observed when the externally controlled limb was identical to the controlled limb (Experiment 1) and when actions of limbs and tools (Experiment 2) or pairs of tools (Experiment 3 and 4) were combined according to participants' motor experience. When participants acted together, controlling a tool each, spatial compatibility effects occurred regardless of motor experience (Experiment 5). These findings demonstrate that (a) externally controlled tool actions are included in action planning and (b) social context modulates how externally controlled actions are mapped onto action representations. Implications of these findings for theories of perception–action links and object processing are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

The 30-minute (1-24)ACTH test for evaluation of adrenocortical function in childhood

Twenty-eight children with normal adrenocortical function and two patients with adrenocortical insufficiency were enrolled in the present study. The ACTH test was performed in these 30 children, and the insulin hypoglycemia test was also performed for the same group, except for one with Addison's disease. Serum cortisol response after intravenous administration of (1-24)ACTH was below normal range in two patients with adrenocortical insufficiency. Neither significant correlation nor statistically significant difference was found between the serum cortisol level 30 minutes after (1-24)ACTH and the peak cortisol value during the insulin hypoglycemia test. None of these children had any adverse reaction to ACTH test. This study suggests that the 30-minute (1-24)ACTH test is a simple, safe and valuable screening procedure for evaluation of adrenocortical function in children.     

Importance of the Arg-Gly-Asp triplet in human thrombin for maintenance of structure and function

Site-directed mutagenesis was employed to assess the importance of the Arg-Gly-Asp triplet that comprises residues 197 to 199 in the B-chain of thrombin. Properties of the R197E and the D199E variants were compared with those of zeta-thrombin and the inactive S205A variant wherein the active site Ser is replaced by Ala. Relative to zeta-thrombin, the R197E thrombin variant under the assay conditions used exhibits 26% activity toward a small chromogenic substrate, 13% activity in the activation of protein C in the presence of thrombomodulin, < 3% activity in processing fibrinogen, and 1% activity in inducing platelet activation. Thus, the substrate specificity of thrombin was altered by the R197-->E replacement. The D199E variant was essentially inactive. It exhibited only 0.02% of the activity of thrombin toward the chromogenic substrate and its reactivity toward the active site-directed alkylating agent D-Phe-Pro-Arg-CH2Cl was 10,000-fold lower than that of thrombin. Like the inactive S205A thrombin variant, the D199E variant antagonized the interactions of thrombin with hirudin and thrombomodulin, but was a less effective antagonist. The dependence of the antagonism of the thrombin-thrombomodulin interaction on the concentration of D199E thrombin variant provided evidence suggesting the presence of two or more domains in thrombin that independently interact with their counterparts in thrombomodulin. Although the S205A thrombin variant antagonized the action of thrombin on platelets no such activity could be demonstrated for the D199E variant in the concentration range studied (< 800 nm). Comparison of the circular dichroism spectra of zeta-thrombin, the D199E, R197E, and S205A variants indicated that subtle differences in conformation exist between the D199E variant and the other thrombins. These differences in conformation might well account for the altered behavior of the D199E variant with respect to its interactions toward thrombomodulin, hirudin, and platelets.     

Video microscopic analysis of ionophore induced acrosome reactions of lobster (Homarus americanus) sperm

Sperm from the American lobster (Homarus americanus) are normally nonmotile. However, during fertilization, the sperm undergo a calcium-dependent acrosome reaction that propels them forward about 18 microns. The reaction occurs in two phases, eversion and ejection, which take place too quickly to permit analysis by direct observation. The purposes of this study were to examine the structural changes occurring in sperm during the normal acrosome reaction and to determine the rate of the reaction using video microscopy. The reaction was induced in vitro by ionophore A23187 and recorded using a video system attached to a Nikon Nomarski interference microscope. Videotapes were played back frame by frame (30 frames/sec), and images of reactions from 10 sperm were analyzed. The acrosome reaction, including the eversion of the acrosomal vesicle and ejection of the subacrosomal material and nucleus, can be divided into 4 steps: (1) expansion of the apical cap followed by expansion of the remainder of the acrosomal cylinder; expansion of the cylinder begins at its apical end and proceeds toward its base, (2) eversion of the apical half of the acrosomal vesicle and initial contraction of the apical cap, (3) eversion of the basal half of the acrosomal vesicle, continued contraction of the apical cap, and ejection of the subacrosomal material and nucleus, and (4) final contraction of the apical cap and ejection of the acrosomal filament. During steps 2, 3, and 4, the mean forward movement of sperm is 12.7, 3.9, and 1.1 microns, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)     

Revision of dysfunctional Tenckhoff catheters by laparoscopic technique in continuous ambulatory peritoneal dialysis patients

A laparoscopic technique was used to rescue dysfunctional Tenckhoff catheters in five continuous ambulatory peritoneal dialysis (CAPD) patients. This method proved to be effective in recovering the function of the catheters, whose malfunction was found to be due to catheter entrapment or endoluminal tamponing by the omentum. The videoscope-assisted laparoscopic technique provides access and allows identification and eradication of the specific etiology leading to catheter drainage failure. It also provides an alternative for treating patients with a dysfunctional Tenckhoff catheter. This method avoids the need to remove the original catheter or to reimplant a new catheter.     

Combination cytotoxic effects of cis-diamminedichloroplatinum(II) and 5-fluorouracil with and without leucovorin against human non-small cell lung cancer cell lines

Both cisplatin (CDDP) and leucovorin (LV) have been shown to enhance cytotoxicity of 5-fluorouracil (FUra) against murine and human neoplasms by increasing intracellular reduced folate concentrations. We were interested in their use in a combination to inhibit non-small cell lung cancer (NSCLC) cell growth and therefore conducted an in vitro study to investigate the cytotoxic activities of combinations of CDDP plus FUra, with and without LV (20 microM), against seven NSCLC cell lines. A tetrazolium assay with application of the classical isobole method was used to test drug combinations. We found that LV enhanced FUra but not CDDP cytotoxicity and that the degree of enhancement was negatively correlated with the effect of FUra. There was an overall additive combination effect of CDDP plus FUra, although there may be synergy at higher effect levels. There was synergy to a combination of CDDP, FUra, and LV, presumably primarily related to the synergistic effects of adding LV to FUra. In summary, LV and CDDP enhanced FUra cytotoxicity in a complementary fashion and there was clear synergy of a combination of CDDP, FUra, and LV against a panel of NSCLC cell lines. Our in vitro results provide a rationale for controlled clinical studies of this three-drug regimen in patients with NSCLC.     

Experiments in term weighting for novelty mining

Obtaining new information in a short time is becoming crucial in today’s economy. A lot of information both offline or online is easily acquired, exacerbating the problem of information overload. Novelty mining detects documents/sentences that contain novel or new information and presents those results directly to users (Tang, Tsai, & Chen, 2010). Many methods and algorithms for novelty mining have previously been studied, but none have compared and discussed the impact of term weighting on the evaluation measures. This paper performed experiments to recommend the best term weighting function for both document and sentence-level novelty mining.     

Robust fuzzy stabilization of dithered chaotic systems using island-based random optimization algorithm

Applying dither to highly nonlinear systems may suppress chaotic phenomena, but dynamic performance, such as convergence rate and disturbance attenuation, is usually not guaranteed. This paper presents a dithered H_∞ robust fuzzy control scheme to stabilize chaotic systems that ensures disturbance attenuation bounds. In the proposed scheme, Takagi-Sugeno (T-S) fuzzy linear models are used to describe the relaxed models of the dithered chaotic system, and fuzzy controllers are designed based on an extension to the concept of parallel distributed compensation (PDC). Sufficient condition for the existence of the H_∞ robust fuzzy controllers is presented in terms of a novel linear matrix inequalities (LMI) form which takes full consideration of modeling error and disturbances, but cannot be solved by the standard procedures. In order to solve the LMI problem and to identify the chaotic systems as T-S fuzzy modes, we propose a compound optimization strategy called the island-based random-walk algorithm (IRA). The algorithm is composed of a set of communicating random-walk optimization procedures concatenated with the down-hill simplex method. The design procedure and validity of the proposed scheme is demonstrated via numerical simulation of the dithered fuzzy control of a chaotic system.     

Femtosecond laser-induced silicon surface morphology in water confinement

This article investigates the use of femtosecond laser induced surface morphology on silicon wafer surface in water confinement. Unlike irradiation of silicon surfaces in the air, there are no laser induced periodic structures, but irregular roughness is formed when the silicon wafer is ablated under water. The unique discovery of a smoothly processed silicon surface in water confinement under certain laser parameter combinations may help improve laser direct micromachining surface quality in industrial applications.     

Lead-lag compensator design by the Hough transform

The frequency response of a compensator can be expressed as a curve in the complex plane. The Hough transform is very useful in finding a mathematical model which matches the curve in a plane very well. We combine these concepts to generate a new method of compensator design. Two examples are illustrated to explain the design procedure and results.