Analysis of simian hemorrhagic fever virus (SHFV) subgenomic RNAs, junction sequences, and 5' leader

Full-length simian hemorrhagic fever virus (SHFV) genome RNA (about 15 kb in length) and six subgenomic RNAs, ranging in size from 0.65 to 4.7 kb, were detected by Northern blot hybridization in MA104 cytoplasmic extracts with a 3' genomic antisense probe. The 5' regions of the two smallest subgenomic RNAs (RNAs 6 and 7) were cloned and sequenced. Sequence analysis indicated that these two RNAs contained a common 5' leader sequence joined to the subgenomic RNA bodies via a highly conserved junction sequence; the junction sequence of RNA 7 was 5'-TTAACC-3', while that of RNA 6 was 5'-TCAACC-3'. The complete 5' leader sequence (208 nt) was obtained from genomic RNA. The genomic 5' junction sequence is identical to that of RNA 7. Northern blot hybridization with an antisense 5' leader probe confirmed the presence of the complete leader sequence in all six species of subgenomic RNA. In its virion morphology, genome size, gene order, and replication strategy, SHFV is most similar to viruses such as equine arteritis virus, lactate dehydrogenase-elevating virus, and Lelystad virus/porcine respiratory and reproductive syndrome virus.     

加入WTO对我国水利科技工作的影响及对策

加入WTO后，对我国水利科技发展的影响主要表现在：研究开发活动、技术标准和知识产权保护方面，为适应WTO的各项规则，履行我国政府所作出的承诺，必须调整水利科技政策和发展战略，促进我国水利科技事业的健康发展。     

一种系统信息提示的方法及其在汇编程序设计中的实现

本文介绍了系统信息提示的一种方法，井重点讨论了用８０８８汇编语言实现的方法。它采用基本信息串表和索引表，对于使用频度较高的系统，可以大大节约系统内存，具存较好的实性用。     

炼油厂区内新建项目改扩建施工现场灭火器配置的分析

在对石油化工改扩建工程施工现场火灾危险因素识别评估的基础上，判定现场火灾危险等级、火灾类别，对灭火级别和保护距离进行验算，提出施工现场灭火器选型和配置方案。     

A force modulation study on crystallization process in ferroelectric vinylidene fluoride and trifluoroethylene copolymer films

This paper introduces the Force Modulation technique to the study of crystallization process in ferroelectric vinylidene fluoride and trifluoroethylene copolymer films. Using this technique we have successfully visualized ferroelectric crystalline domains and observed that these ferroelectric domains grow out from amorphous phase, unite into strip-like structures, and finally congregate into a union. Force Modulation can weaken the influence of topography on imaging of ferroelectric domains, and reveal more details, which are difficult to be observed in topographical image.     

冷却排管的节能设计

从节能的观点出发，根据热力学原理和传递过程的理论，对冷却排管内湍流传热过程进行分析，研究损失随雷诺数的变化规律，探讨冷却排管的节能设计问题。     

影响聚合物段塞流变性因素的研究

本文针对我国表面活性剂/聚合物驱油的实际,研究了石油磺酸盐(PS)、正丁醇(R_4OH)和矿化水对水解聚丙烯酰胺(HPAM)溶液流变性的影响。结果表明,PS对HPAM溶液流变性的影响与介质的矿化度有关:非矿化水介质中,PS对HPAM溶液性质的影响类似于无机盐,能降低HPAM溶液的表现粘度(η_α)和稠度系数(k);当系统中含有一定浓度矿化水时,PS却能升高系统的η_α和k值,降低流型指数(n),使系统的非牛顿性增强。在含有PS和矿化水的HPAM系统中加入R_4OH,系统的η_α和k值会有所增加。     

预测控制算法的统一格式及其在电加热炉的应用

本文根据常用的两类预测控制算法推出一种新型预测控制算法。并将其应用于一双输入/双输出的力学持久机电加热炉的温度控制系统上,获得了满意的结果:控温精度为±1.5℃/300℃,且适用于非最小相位系统。     

Procedures for analysis of strain transfer in the acetabular region in vitro

The failure of acetabular cups via loosening, migration or fracture is partially related to the stress distribution existing in the acetabular region. The complexity of the investigation of these stresses demands the application of a variety of model testing techniques involving combined embedded strain gauging, finite element analysis, transmission and reflective photoelasticity and crack propagation studies. The application of these procedures are described and the difficulties of obtaining representative loadings and restraints are commented upon.     

浅析深层搅拌桩的材料使用

本文就深层搅拌桩施工材料使用存在的一些问题进行分析总结。