Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis

To identify potential biomarkers for improving diagnosis of melioidosis, we compared plasma metabolome profiles of melioidosis patients compared to patients with other bacteremia and controls without active infection, using ultra-high-performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry. Principal component analysis (PCA) showed that the metabolomic profiles of melioidosis patients are distinguishable from bacteremia patients and controls. Using multivariate and univariate analysis, 12 significant metabolites from four lipid classes, acylcarnitine (n = 6), lysophosphatidylethanolamine (LysoPE) (n = 3), sphingomyelins (SM) (n = 2) and phosphatidylcholine (PC) (n = 1), with significantly higher levels in melioidosis patients than bacteremia patients and controls, were identified. Ten of the 12 metabolites showed area-under-receiver operating characteristic curve (AUC) >0.80 when compared both between melioidosis and bacteremia patients, and between melioidosis patients and controls. SM(d18:2/16:0) possessed the largest AUC when compared, both between melioidosis and bacteremia patients (AUC 0.998, sensitivity 100% and specificity 91.7%), and between melioidosis patients and controls (AUC 1.000, sensitivity 96.7% and specificity 100%). Our results indicate that metabolome profiling might serve as a promising approach for diagnosis of melioidosis using patient plasma, with SM(d18:2/16:0) representing a potential biomarker. Since the 12 metabolites were related to various pathways for energy and lipid metabolism, further studies may reveal their possible role in the pathogenesis and host response in melioidosis.     

"智慧消防"在防火监督中的应用探究

随着科学技术的发展和经济水平的提高,人们对消防安全的认识日益增强.需要重视消防安全,做好消防管理,并采用先进的管理模式,在当前的消防管理模式下应用"智能消防",可以确保人民生命财产安全提供强有力的支持,使现代科学技术可以更好地融入到消防工作中,不仅可以提高火灾监控能力,还可以提高消防水平,对消防安全领域的发展具有重要意义,使传统消防逐步向智能消防转变,并不断升级.     

ZWB-10（6.3）kV线路无功功率自动补偿器的使用

采油二厂现有30条6 kV配电线路,每条线路的功率因数各不相同,有的达到标准,有的没有达到标准。众所周知,如果线路的功率因数低,就会造成线路电流增大,电能损耗增加,用电量也随之增加,电量的增加也就带来了企业成本的增加。因此,提高配电线路的功率因数是目前所有供电部门的一项非常重要的工作。     

不锈钢酸洗污泥微波场中升温特性的研究

本文就酸洗污泥微波场中的升温特性进行了系统研究,旨在为探求酸洗污泥微波辐射深度脱水新工艺提供理论依据.结果表明,酸洗污泥干泥在微波场中的升温特性为先缓慢升温至拐点温度后开始快速升温;酸洗污泥湿泥在微波场中的升温特性为先快速升温至110℃左右后开始缓慢升温,直至水分蒸发完才出现拐点温度并开始快速升温.     

Formation and microstructural characterization of whey protein isolate/beet pectin coacervations by laccase catalyzed cross-linking

The purpose of this study was to characterize the properties of stable and reinforced protein–polysaccharides complex coacervations firstly formed by electrostatic interaction between WPI and beet pectin, and followed by laccase cross-linking through ferulic acid present in beet pectin. The interaction of whey protein isolate (WPI, 6 g/100 ml) with beet pectin (0–0.16 g/100 ml) in aqueous solutions was studied at different pH (3–7). ζ-potential and light-scattering techniques were used to provide information about the electrical charge and aggregation of individual biopolymers and complex coacervations. Stable WPI/beet pectin complex coacervations were formed when system consisted of 6 g/100 ml WPI and 1 g/100 ml beet pectin at pH 3.5. The microstructure and viscoelastic properties of WPI and beet pectin complex coacervations in the presence of laccase (0, 100, 300 U) was also studied using FT-IR, rheology, and confocal laser scanning microscopy (CLSM) techniques. The results obtained clearly showed that laccase catalyzed cross-linking of ferulic acid present in beet pectin had a remarkable influence on the physical properties of WPI–beet pectin complex coacervations microstructure. The reinforced complex coacervations formed fine networking structures which may provide convenient means for food industry to incorporate bioactive components into food products.     

酒中乙醇浓度国标检测方法的改进

国标GB 5009.225-2016《食品安全国家标准酒中乙醇浓度的测定》第二法为酒精计法,标准方法将制备好的试样注入100m L或200m L量筒中用分度值为0.1%vol的精密酒精计读取酒精度值,在实际实验中无法实现该操作。本文通过对该国标方法的改进,能准确、高效的完成酒精计法测定酒中乙醇浓度。     

GIS技术在城市防洪排涝中的应用分析

随着城镇化进程不断加快以及气候变暖带来的极端气候变化,部分城市接连遭受大型洪涝灾害。暴雨洪涝在引起媒体与公众普遍关注的同时,也给城市管理部门的防御和对策研究带来了新的难题。GIS技术在空间信息分析上的广泛应用为城市应对汛期洪涝灾害提供了新方法和新思路。     

工业二氧化碳废气用于农作物增产的绿色减排途径

随着经济增长,结构转型,以及履行负责任大国的国际义务越来越重等因素,中国现在面临着节约能源、保护环境和减少温室气体排放的多重压力。主要论述了二氧化碳应用农业生产方面,既能减少碳的排放量,又能提高农作物产量,从而实现生态、农业双赢的局面。