Characterization,Expression Profiling,and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants

Cinnamoyl-CoA reductase (CCR) is a pivotal enzyme in plant lignin synthesis, which has a role in plant secondary cell wall development and environmental stress defense. Alfalfa is a predominant legume forage with excellent quality, but the lignin content negatively affects fodder digestibility. Currently, there is limited information on CCR characteristics, gene expression, and its role in lignin metabolism in alfalfa. In this study, we identified 30 members in the CCR gene family of Medicago sativa. In addition, gene structure, conserved motif, and evolution analysis suggested MsCCR1–7 presumably functioned as CCR, while the 23 MsCCR-likes fell into three categories. The expression patterns of MsCCRs/MsCCR-likes suggested their role in plant development, response to environmental stresses, and phytohormone treatment. These results were consistent with the cis-elements in their promoters. Histochemical staining showed that lignin accumulation gradually deepened with the development, which was consistent with gene expression results. Furthermore, recombinant MsCCR1 and MsCCR-like1 were purified and the kinetic parameters were tested under four substrates. In addition, three-dimensional structure models of MsCCR1 and MsCCR-like1 proteins showed the difference in the substrate-binding motif H212(X)2K215R263. These results will be useful for further application for legume forage quality modification and biofuels industry engineering in the future.     

科技论文撰写系列讲座(终)——结论、参考文献与最终检查

0 引言 以上5讲,第1讲是介绍如何撰写综述文章,其他4讲分别介绍了论文材料准备;题目、摘要的写法;关键词与正文的写法:图表的处理.本讲是全部讲座的最后一讲,主要谈谈结论、参考文献与论文的最终检查. 经过了前几讲的介绍,想必作者已经写出了较好的论文.在即将投稿的时候,请作者千万别忘了这最后的画龙点睛的部分.本讲按照顺序分以下3个部分讲述.     

基于海洋背景红外图像的仿真系统研究

简要介绍了一种简便的基于海洋背景的红外仿真系统的设计和组成,阐述了各个模块的功能以及模块之间的联系。在实际存储红外图像素材基础上,通过直接添加模板图像,接着通过后续的处理模块来实现较好的模拟海洋红外图像中的目标的目的。从最后的结果可以看到这是一个简便有效的仿真系统。     

Phase characterisation of metalenses

Metalenses have emerged as a new optical element or system in recent years, showing superior performance and abundant applications. However, the phase distribut...     

Characteristic strength and acoustic emission properties of weakly cemented sandstone at different depths under uniaxial compression

As coal mining is extended from shallow to deep areas along the western coalfield,it is of great significance to study weakly cemented sandstone at different de...     

Effect of Controlled Cooling After Hot Rolling on Mechanical Properties of Hot Rolled TRIP Steel

A three-step cooling pattern on the runout table（ROT）was conducted for the hot rolled TRIP steel.Microstructural evolution during thermomechanical controlled processing（TMCP）was investigated.Processing condition of controlled cooling on a ROT in the laboratory rolling mill was discussed.The results indicated that the microstructure containing polygonal ferrite,granular bainite and a significant amount of the stable retained austenite can be obtained through three-step cooling on the ROT after hot rolling.TMCP led to ferrite grain refinement.Controlled cooling after hot rolling resulted in the stability of the remaining austenite and a satisfactory TRIP effect.Excellent mechanical properties were obtained through TMCP for the hot rolled TRIP steel.     

静压PHC管桩施工问题分析与讨论

静压PHC管桩在福建省内广泛采用,本文描述静压PHC管桩在施工中常见的问题及处理方法,并以四季康城为施工实例,介绍其施工技术和质童控制,给相关部门提供参考,提高我国静压PHC管桩的管理水平。     

复杂流场的激光—超声测试方法及原理性实验研究

本文从复杂流场-旋涡场参量的激光-超声测试方法的需要出发,论述了超声波产生的声位相光栅对激光产生的衍射及偏转特性。在水中进行了声光偏转等试验,测出了产生声光偏转时折射率的变化及声压变化,从而估算出空气复杂流场激光——超声方法所需的超声频率及能量,为实现空气流场激光——超声无接触测试的研究提供了依据。     

激光二极管抽运Nd∶YVO4/YVO4复合晶体激光器

在高功率激光二极管(LD)抽运的情况下,对比分析了Nd∶YVO4/YVO4复合晶体和Nd∶YVO4单一晶体的激光特性。实验证明,复合晶体能够有效地降低晶体内的温度梯度,减小由端面变形带来的热透镜效应,获得比单一晶体高出许多的输出功率。采用Z型折叠腔,研究了Nd∶YVO4/YVO4复合晶体KTP倍频特性,当抽运功率为17W时,获得了6.23W的绿光输出,抽运光到绿光的转换效率高达37%。     

建设工程施工图审查信息系统的分析与设计

以建设工程施工图审查信息系统的开发为原型,针对目前信息系统开发中遇到的问题,采用UML建模技术完成本系统的分析与设计。结合UML用例图、活动图、时序图、类图来详细分析UML在信息系统分析、设计等阶段中的建模应用。基于文中设计方案所完成的系统已投入实际运行,实践证明,文中的研究对加快系统开发速度,提高系统可靠性起到了良好的作用,达到了预期效果。