厌氧活性污泥PCR-DGGE图谱的优化

针对特种污泥PCR-DGGE图谱的优化一直是微生物分子生态学研究的重点.在反硝化抑制硫酸盐还原菌的研究中,研究厌氧硫酸盐还原污泥和反硝化污泥DNA的提取方法,应用通用引物958F/1401R扩增16S rDNA序列,探讨不同的退火温度对DGGE指纹图谱多样性影响,同时采用时间进程法对DGGE图谱进行优化.结果表明,采用PBS洗涤污泥和超声震荡有利于硫酸盐还原污泥DNA提取;采用958F/1401R为引物时,扩增片段大约500 bp,不同的退火温度对DGGE指纹图谱的多样性影响不显著;采用时间进程法可以很好地再现不同电泳阶段对样品的分离效果,最佳变性剂梯度为40%～60%,在130 V电压下,最佳电泳时间为5 h.     

Effects of main factors on remedying northern landscaping water by hybrid ecological filter

Natural zeolite and coal cinder were layered as main packing medium of the ecological filter instead of traditional filling to remedy the static lake water in Northern China.The ecological filter was running in a combined upward and downward flow mode.Dynamic experiments were carried out to study the effects of retention time and ambient temperature on pollutants’ removal efficiency of the hybrid ecological filter.The function of plant was also studied by contrast test.It is showed that the removal efficiencies of NH4+-N and TP are increased when the retention time is changed from 1 h to 2 h and 4 h,but the removal efficiency of TN is decreased,the removal efficiency of NH4+-N is increased from 91.5% to 98% and that of TP is increased from 31.8% to 52.5%.When the temperature declines,the temporal removal efficiency of NH4+-N is reduced,but the removal efficiency of 24 h and 48 h is remained.The removal efficiency of TP after 24 h and 48 h is decreased when the temperature declines evidently.The retention time plays an important role in NH4+-N and TP removal,and the ambient temperature is significant for TP removal.The plant favors for TP and organic matter removal but has little effect on TN removal.     

基于模糊神经网络的锅炉汽包水位控制器的设计

将模糊网络理论引入锅炉汽包水位控制系统中,以解决水位稳定性控制的问题,针对DSP具有高速运算能力的特点,采用TMS320LF2407对数据进行采集、运算处理,试验结果证明了其时效性.     

可拓集合论在专家系统知识表示中的应用

引入了可拓集合论来优化专家系统的设计过程,阐述了可拓集合论应用于专家系统的可行性,在分析知识表示的集合与分类特征的基础上,探讨了可拓集合论在知识表示中的应用,结合人工智能技术的发展趋势,对专家系统在可拓学中的应用前景做出了展望.     

SMA-5应力吸收层在旧水泥路面加铺改造中的应用研究

为减少和延缓旧水泥板沥青加铺层反射裂缝的产生,在分析研究各种夹层材料应用效果和SMA-5作为应力吸收层性能要求的基础上,设计了SMA-5应力吸收层的配合比,采用搓揉成型的方法改进了马歇尔试件,并对SMA-5沥青混合料的各项路用性能进行了试验研究,且成功的铺筑了试验路.结果表明:SMA-5沥青混合料可以作为夹层材料,并起到了很好的应力吸收效果.此项研究成果对于旧水泥混凝土路面加铺改造具有重要的工程实用价值,SMA-5应力吸收层具有广阔的应用前景.     

Solvent free oxidation of alcohols catalyzed by KMnO4 adsorbed on Kieselguhr

Efficient and selective oxidation of primary and secondary alcohols to the corresponding aldehydes and ketones catalyzed by potassium permanganate supported on Kieselguhr reagent under solvent free conditions are reported.     

A Novel Filtering Based Recursive Estimation Algorithm for Box-Jenkins Systems

International Journal of Control, Automation and Systems - This paper presents a novel filtering based multi-innovation estimation algorithm for output-error autoregressive moving average (i.e.,...     

Reaction mechanism and microstructure evolution of reaction sintered <Emphasis Type="Italic">h</Emphasis>-BN

Hexagonal boron nitride ceramic (h-BN) based on the nitridation of B powders was obtained by reaction sintering method. The effects of sintering temperature on the mechanical properties and microstructure of the resultant products were investigated and the reaction mechanism was discussed. Results showed that the reaction between B and N₂ occurred vigorously at temperatures ranging from 1 000 °C to 1 300 °C, which resulted in the generation of t-BN. When the temperature exceeded 1 450 °C, transformation from t-BN to h-BN began to occur. As the sintering temperature increased, the spherical particles of t-BN gradually transformed into fine sheet particles of h-BN. These particles subsequently displayed a compact arrangement to achieve a more uniform microstructure, thereby increasing the strength.     

Effect of ambient wind on pressure wave generated by high-speed train entering a tunnel

Journal of Central South University - Using three-dimensional, unsteady N-S equations and k-ε turbulence model, the effect of ambient wind on the pressure wave generated by a high-speed train...