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The method of reversed-phase high performance liquid chromatography was established for the determination of effective components in OC-CS spray using double wavelength UV detection.The method was utilized under following conditions:a column of Kromasil C18(250 mm × 4.6 mm,5 μm),mobile phase consisting of methanol/water(80 /20) at a flow rate of 0.8 mL/min,column temperature of 25 ℃,and the UV detection at 227 nm and 300 nm.Three key components in OC-CS spray could be distinguished clearly,including o-chlor... 相似文献
43.
生物分离工程是整个生物产业技术中的关键环节,而生物分离工程实验课程是生物工程专业学位教育中不可替代的内容。本文阐述了利用具有不同专业知识基础的专业学位硕士生的知识基础不同,选择不同实验内容。在实验设计中,强调知识点的模块化设计。通过学生参与设计,调动学生学习积极性。开展好实验设计,对培养专业型人才有重要作用。 相似文献
44.
Molecular imprinting technology has a great potential to be used in protein separation and purification.In this work,lysozyme imprinted polyacrylamide gel was prepared with silica particles as a sacrificial template to generate macro-porosity for fast adsorption.The adsorption equilibrium time and adsorption capacity were 9 h and 56 mg/g respectively,which was 2 h less and 2.3-fold more than polymers without the sacrificial template.In order to test molecular imprinting polymers(MIPs)'selectivity,bovine serum albumin(BSA)was chosen as interferent for binary adsorption tests.In addition,the adsorption selectivity was further investigated using different molar ratios of lysozyme to BSA with fixed total concentration of proteins,as well as using various total concentrations of proteins with an equimolar ratio of lysozyme to BSA.It has been proven that the total concentration of proteins should be larger than1.5×10~(-7) mol/mL,when the molar ratio of BSA to Lyz is 1∶1,in order to effectively separate Lyz from the binary protein mixture.The macro-porous lysozyme molecularly imprinted polymers have less adsorption time,larger adsorption capacity,and better imprinting effect. 相似文献
45.