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71.
72.
Horizontal Dip‐Spin Casting of aqueous alumina‐polyvinylpyrrolidone suspensions with chopped fiber
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Valerie Wiesner Manuel Acosta Lisa Rueschhoff Jeffrey Youngblood Rodney Trice 《International Journal of Applied Ceramic Technology》2017,14(6):1077-1087
A novel ceramic processing method, called Horizontal Dip Spin Casting (HDSC), enabled fabrication of tubular ceramic parts with an aligned chopped fiber phase. HDSC was demonstrated using highly loaded aqueous alumina suspensions with >50 vol.% solids loading and ≤5 vol.% water‐soluble polymer employed as a rheological modifier. Chopped carbon fibers were added to the suspensions to attain maximum loadings of 30 vol.%. During forming, cylindrical foam molds were dipped into the suspension while being rotated radially about the long axis. Simultaneously, a doctor blade was placed at a specified distance from the foam surface to facilitate the flow of the suspension to align the fiber and control the thickness of the material that accrued on the mold. Rheological study of alumina‐PVP suspensions with and without chopped carbon fiber showed that the suspensions exhibited a yield‐pseudoplastic flow behavior. The degree of alignment of the carbon fiber phase in the green bodies was characterized for various suspension formulations, carbon fiber contents and forming speeds. Stereological characterization of green body specimens confirmed the effectiveness of HDSC to attain the desired tubular geometry with considerable fiber alignment for a suspension composition containing ≤20 vol.% chopped fibers. 相似文献
73.
Laura Frutos-Rincn Jos Antonio Gmez-Snchez Almudena Íigo-Portugus M. Carmen Acosta Juana Gallar 《International journal of molecular sciences》2022,23(6)
The cornea is an avascular connective tissue that is crucial, not only as the primary barrier of the eye but also as a proper transparent refractive structure. Corneal transparency is necessary for vision and is the result of several factors, including its highly organized structure, the physiology of its few cellular components, the lack of myelinated nerves (although it is extremely innervated), the tightly controlled hydration state, and the absence of blood and lymphatic vessels in healthy conditions, among others. The avascular, immune-privileged tissue of the cornea is an ideal model to study the interactions between its well-characterized and dense sensory nerves (easily accessible for both focal electrophysiological recording and morphological studies) and the low number of resident immune cell types, distinguished from those cells migrating from blood vessels. This paper presents an overview of the corneal structure and innervation, the resident dendritic cell (DC) subpopulations present in the cornea, their distribution in relation to corneal nerves, and their role in ocular inflammatory diseases. A mouse model in which sensory axons are constitutively labeled with tdTomato and DCs with green fluorescent protein (GFP) allows further analysis of the neuro-immune crosstalk under inflammatory and steady-state conditions of the eye. 相似文献
74.
C. Magaña-Zavala M. E. Angeles-San Martin F. J. Rodríguez-Gómez D. R. Acosta R. Avila-Godoy A. López-Suárez 《Surface Engineering and Applied Electrochemistry》2010,46(2):115-119
Nickel thin films with regular configuration and similar thickness were deposited on steel AISI 1018 (UNSG 10180) by two different
techniques: magnetron sputtering and electrolytic process. The main aim of this work is to compare the surfaces deposited
films made by the two techniques using the scanning electronic microscopy and to identify their morphological differences
and imperfections. We evaluate the protective coatings properties when the films are in contact with an acid medium. We also
study the thin films coatings by the spectroscopy impedance technique. We obtain that the sputtering deposits present a homogeneous
thin film coating that is better than the one obtained by the electrolytic technique. It is expected that the thin film coatings
made in this work can protect the steel against corrosion when it is in contact with an acid environment. 相似文献
75.
Interconnection and damping assignment passivity-based control of mechanical systems with underactuation degree one 总被引:1,自引:0,他引:1
Acosta J.A. Ortega R. Astolfi A. Mahindrakar A.D. 《Automatic Control, IEEE Transactions on》2005,50(12):1936-1955
Interconnection and damping assignment passivity-based control is a new controller design methodology developed for (asymptotic) stabilization of nonlinear systems that does not rely on, sometimes unnatural and technique-driven, linearization or decoupling procedures but instead endows the closed-loop system with a Hamiltonian structure with a desired energy function-that qualifies as Lyapunov function for the desired equilibrium. The assignable energy functions are characterized by a set of partial differential equations that must be solved to determine the control law. We prove in this paper that for a class of mechanical systems with underactuation degree one the partial differential equations can be explicitly solved. Furthermore, we introduce a suitable parametrization of assignable energy functions that provides the designer with a handle to address transient performance and robustness issues. Finally, we develop a speed estimator that allows the implementation of position-feedback controllers. The new result is applied to obtain an (almost) globally stabilizing scheme for the vertical takeoff and landing aircraft with strong input coupling, and a controller for the pendulum in a cart that can swing-up the pendulum from any position in the upper half plane and stop the cart at any desired location. In both cases we obtain very simple and intuitive position-feedback solutions. 相似文献
76.
Pretreatment of mice with clofibrate (CFB) has been shown to protect against acetaminophen (APAP) hepatotoxicity. To determine if pretreatment with CFB prevents the toxicity of other model hepatotoxicants, male C57BL6J or CD-1 mice received 500 mg CFB/kg, i.p., daily for 10 days, and then were challenged with either 250 mg bromobenzene (BrB)/kg, 0.025 ml carbon tetrachloride (CCl4)/kg or 0.5 ml chloroform (CHCl3)/kg. Liver and kidney injury was assessed by plasma sorbitol dehydrogenase activity (SDH) and blood urea nitrogen (BUN), respectively and histopathology. Challenge with BrB significantly elevated plasma SDH activity in C57Bl6J mice. This was prevented in CFB pretreated mice receiving the same dose of BrB. Changes in BUN were not detected in either group of BrB treated mice. Similarly, pretreatment of male CD-1 mice with CFB significantly reduced CCl4-induced elevation in plasma SDH activity, with no BUN elevation detected in either group. CFB pretreatment also diminished elevation in plasma SDH activity produced by CHCl3 in CD-1 mice, while BUN was significantly elevated in both groups, indicating that CFB did not protect against CHCl3-induced nephrotoxicity. Histopathological examination of liver and kidney sections confirmed these results. This study shows that mice pretreated with CFB were protected from toxicity at 24 h after challenge with other model hepatotoxic agents besides APAP. 相似文献
77.
VM Borderie M Lopez A Lombet S Carvajal-Gonzalez C Cywiner L Laroche 《Canadian Metallurgical Quarterly》1998,39(8):1511-1519
PURPOSE: To assess the effects of two different concentrations of albumin in a cryoprotective solution and two freezing methods on human corneal keratocyte ctyopreservation. METHODS: Isolated keratocytes were used for cryopreservation. Solutions of 10% dimethylsulfoxide with either 2% or 10% human albumin were used as cryoprotective agents. Cells either were transferred directly into a -80 degrees C freezer (freezing rate, 2 degrees C/min) or were cooled in a programmed freezer (1 degrees C/min until -40 degrees C and then 10 degrees C/min), which resulted in four different cryopreservation protocols. Cells were stored at -80 degrees C, then were thawed at 37 degrees C, and subsequently were cultured. Keratocytes were studied by means of trypan blue staining, growth assay, apoptosis assays, transmission electron microscopy, and immunochemistry. RESULTS: The percentage of cells that were alive after thawing ranged from 80% to 99% by trypan blue staining and from 45% to 60% by flow cytometry. The ratio of the number of living cells at the end of primary culture after cryopreservation to that before cryopreservation was significantly (P=0.04) higher after direct transfer into the -80 degrees C freezer than after controlled-rate freezing, whereas the albumin concentration had no significant influence on this ratio (P=0.45). The percentage of apoptotic cells was significantly higher after cryopreservation than in the control group of noncryopreserved cells; more than 5% 24 hours after thawing. Cryopreservation did not modify the keratocyte ultrastructure. Fibroblast growth factor dramatically decreased the serum-induced cell expression of alpha smooth muscle actin, whereas cryopreservation had no influence on this cell expression. CONCLUSIONS: A freeze-thaw trauma, which was related to cryopreservation-induced cell apoptosis, was revealed during primary culture after thawing. Direct transfer into the -80 degrees C freezer resulted in better postcryopreservation growth in the culture than controlled-rate freezing. A change in albumin concentration from 2% to 10% did not affect the results. 相似文献
78.
A dynamic event tree method for analyzing the risk associated with dynamic nuclear power plant accident sequences is presented. The method provides a framework for treating stochastic variations in operating crew states (defined by substrates characterizing the accident diagnosis, the planned actions, and the crew quality) as well as stochastic variations in hardware states. Plant process variables are treated deterministically; they are used when determining the likelihood of stochastic branchings. The method is used in an analysis of a steam generator tube rupture (SGTR) accident; it is shown that a number of important operator behavior patterns can be reasonably represented, and that, comparing with conventional event trees, sources of dependencies between failure events can be better defined. 相似文献
79.
MA Antonysamy RJ Steptoe A Khanna WA Rudert VM Subbotin AW Thomson 《Canadian Metallurgical Quarterly》1998,160(8):4106-4113
C3H (H2k) mice received 50 x 10(6) B10 (H2b) bone marrow (BM) cells either alone or with flt-3 ligand (FL) (10 microg/day), tacrolimus (2 mg/kg/day), or both agents for 7 days. Donor MHC class II+ (IAb+) cells were quantitated in spleens by immunohistochemical analysis, and donor class II DNA detected in BM by PCR. Donor cells were rare in the BM alone and BM + FL groups, whereas there was a substantial increase in chimerism in the BM + tacrolimus group. Addition of FL to BM + tacrolimus led to a further eightfold increase in donor cells and enhanced donor DNA compared with the BM + tacrolimus group. This increase in donor cells was almost 500-fold compared with BM alone. C3H recipients of B10 heart allografts given perioperative B10 BM and tacrolimus (days 0-13) exhibited a markedly extended median graft survival time (MST, 42 days) compared with those given tacrolimus alone (MST, 22 days). Addition of FL (10 microg/day; 7 days) to BM + tacrolimus prevented the beneficial effect of donor BM (MST, 18 days). BM alone or BM + FL resulted in uniform early heart graft failure (MST < 8 days). Functional studies revealed maximal antidonor MLR and CTL activities in the BM- and BM + FL-treated groups, with minimal activity in the tacrolimus-treated groups. Thus, dramatic growth factor-induced increases in chimerism achieved under cover of immunosuppression may result in augmented antidonor T cell reactivity and reduced graft survival after immunosuppressive drug withdrawal. With FL, this may reflect striking augmentation of immunostimulatory dendritic cells. 相似文献
80.
VM Petrov 《Canadian Metallurgical Quarterly》1998,32(5):46-51
The concept of acceptable risk is considered in the context of the system of medical support to space missions. Risk is defined as probability of cosmonaut's health and life deterioration as a result of spaceflight impacts on the human body. Mathematical formulas giving the general characteristic of the relations among risk components, total risk, and human survivability in flight and after its end. The earlier published quantitative recommendations for these parameters are inserted as illustration. 相似文献