Contribution of laser microdissection-based technology to proteomic analysis in hepatocellular carcinoma developing on cirrhosis

Hepatocellular carcinoma (HCC) is a major cause of cancer worldwide. Proteomic studies provide opportunities to uncover targets for the diagnosis and treatment of this disease. However, in HCC developing in a setting of cirrhosis, the detection of proteome alterations may be hampered by the increased cellular heterogeneity of tissue when analysing global liver homogenates. The aim of this study was to evaluate whether the identification of proteome alterations in these HCC cases was improved when the differential protein profile between tumour and non-tumour areas of liver was determined using hepatocytes isolated by laser microdissection (LM). Differential profiles established with LM-hepatocytes and liver section homogenates using 2-DE and MS exhibited noticeable differences: 30% of the protein spots with deregulated expression in tumorous LM-samples did not display any modification in homogenates; conversely 15% of proteins altered in tumorous homogenates were not impaired in LM-hepatocytes. These alterations resulted from the presence in cirrhotic liver of fibrotic stroma which displayed a protein pattern different from that determined in LM-hepatocytes. In conclusion, our data demonstrate the interest of LM in distinguishing between fibrotic and hepatocyte proteome alterations and thus the benefit of LM to proteome studies of HCC developing in a context of cirrhosis.     

Étude de la précision de l’estimation de la densité spectrale de puissance croisée,obtenue par transformation de fourier de la fonction de corrélation

The authors compare two different estimators of the crosscorrelation function and show that they give equivalent estimators in the frequency domain. They calculate the first and second order moments of the estimator of the cross-spectral-density (DSPC)due to the statistical errors and the quantification of inputs. From these general formulas, they perform numerical calculations for a particular case and they compare them to practical measurements. They show the interesting fact, that the variance of the statistical errors on the dspc is inferior by estimating its phase than by estimating its module. Furthermore, the quantification of the inputs may influence the variance of the phase of the DSPC estimated.     

Methodology for the reliability evaluation of a passive system and its integration into a Probabilistic Safety Assessment

A methodology has been developed to evaluate the reliability of passive systems characterised by a moving fluid and whose operation is based on thermal–hydraulic (T-H) principles. The methodology includes:

• identification and quantification of the sources of uncertainties and determination of the important variables;

• propagation of the uncertainties through T-H models and assessment of T-H passive system unreliability;

• introduction of passive system unreliability in the accident sequence analysis.

Each step of the methodology is described and commented and a diagram of the methodology is presented. An example of passive system is presented with the aim to illustrate the possibilities of the methodology. This example is the Residual Passive heat Removal system on the Primary circuit (RP2), an innovating system supposed to be implemented on a 900 MWe Pressurized Water Reactor.     

基于遗传算法的分布式异构数据库的查询优化

对于分布武异构数据库，查询优化问题是其核心问题。本文用基于位、值结构树型编码的遗传算法对分布武异构数据库进行查询优化，取得了很好的效果。     

Useful nonlinearities and global stabilization of bifurcations in amodel of jet engine surge and stall

Compressor stall and surge are complex nonlinear instabilities that reduce the performance and can cause failure of aircraft engines. We design a feedback controller that globally stabilizes a broad range of possible equilibria in a nonlinear compressor model. With a novel backstepping design we retain the system's useful nonlinearities which would be cancelled in a feedback linearizing design. The design control law is simple and, moreover, it is optimal with respect to a meaningful nonquadratic cost functional. As in a previous bifurcation-theoretic design, we change the character of the bifurcation at the stall inception point from subcritical to supercritical. However, since we approach the bifurcation control using Lyapunov tools, our controller achieves not only local but also global stability. The controller requires minimal modeling information and simpler sensing (rotating stall is stabilized without measuring its amplitude)     

Residual stress-affected diffusion during plasma nitriding of tool steels

Plasma nitriding of tool materials is common practice to improve the wear resistance and lifetime of tools. Machining-induced compressive residual stresses in shallow layers of some tenths of microns are observed accompanied by other characteristic properties of machined surfaces in these high-strength materials. After plasma nitriding of M2 high-speed steel, previously induced compressive residual stresses remain stable and the depth of diffusion layers decreases with increasing compressive residual stresses. This article reports investigations of plasma nitrided samples with different levels of residual stresses induced prior to the nitriding process. For comparison, experiments with bending load stresses during plasma nitriding have also been carried out. The plasma nitriding treatment was performed at constant temperature of 500 °C with a gas mixture of 5 vol pct N₂ in hydrogen. Nitriding time was varied from 30 to 120 minutes. All samples were characterized before and after plasma nitriding concerning microstructure, roughness, microhardness, chemical composition, and residual stress states. Experimental results are compared with analytical calculations on (residual) stress effects in diffusion and show a clear effect of residual and load stresses in the diffusion of nitrogen in a high-strength M2 tool steel.     

Fatigue of bituminous mixtures

This paper presents an interlaboratory test campaign organized by the RILEM 182-PEB Technical Committee. In the campaign, 11 different test methods, comprising uniaxial tension/compression, 2-, 3- and 4-point bending and indirecttension tests, were utilized in order to investigate fatigue characteristics of a dense graded asphalt concrete mixture. The testing conditions specified were sinusoidal excitation at 10Hz and 10°C using controlled strain and stress modes. In total, more than 150 fatigue tests were carried out during the investigation. The fatigue test results were analyzed using both classical as well as continuum damage mechanics approaches. The fatigue test results obtained using the classical fatigue approach are considerably influenced by test type and mode of loading (controlled stress or strain) used. Consequently, this approach has limited use in realistic fatigue characterization of bituminous materials and pavement structures. In contrast to the classical approach, models founded on continuum damage theory may serve to isolate intrinsic fatigue characteristics from the influence of so-called biased effects, which are largely caused by the accelerated laboratory testing. The continuum damage models investigated may constitute steps, towards a rational mechanistic fatigue characterization model, which are important for effective future pavement design.     

Childhood Peer Rejection and Aggression as Predictors of Adolescent Girls' Externalizing and Health Risk Behaviors: A 6-Year Longitudinal Study.

This 6-year longitudinal study examined girls' peer-nominated social preference and aggression in childhood as predictors of self- and parent-reported externalizing symptoms, substance use (i.e.. cigarette, alcohol, and marijuana use), and sexual risk behavior in adolescence. Participants were 148 girls from diverse ethnic backgrounds, who were initially assessed in Grades 4-6 and again in Grades 10-12. Results supported a moderator model, indicating that social preference changed the nature of the association between childhood aggression and adolescent outcomes. When accompanied by peer rejection, aggressive behavior was moderately stable over time and significantly associated with adolescent girls' substance use and sexual risk behavior. However, under conditions of peer acceptance, no significant association between childhood aggression and adolescent outcomes emerged. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Environmental Fatigue and Static Behavior of RC Beams Strengthened with Carbon-Fiber-Reinforced Polymer

Many countries around the world have tremendous needs to repair and strengthen their transportation infrastructure. Almost everywhere, traffic loads have reached levels largely exceeding design expectations. Northern countries also experience severe winter conditions that are combined with an extensive use of deicing salts and accelerate structural deterioration. In Canada, the extent of deterioration has prompted many authorities, including the federal and provincial governments, to investigate the potential use of fiber-reinforced polymer products to extend the life of their existing structures. However, it is widely recognized that the large-scale implementation of these products is often impaired by the lack of data on their durability. This paper presents an experimental project undertaken in order to assess the durability of reinforced concrete beams externally strengthened with two types of carbon-fiber-reinforced polymer (CFRP). The beams were first exposed to either wet-dry cycles or continuous immersion in water and then were loaded in fatigue. Finally, they were tested quasi-statically under four-point bending up to failure. The test results presented here provide some insights on the potential long-term performance of CFRP-strengthened beams exposed to severe environmental conditions.