Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea

Phosphopantetheinyl transferases (PPTases) catalyze the essential post-translational activation of carrier proteins (CPs) from fatty acid synthases (FASs) (primary metabolism), polyketide synthases (PKSs), and non-ribosomal polypeptide synthetases (NRPSs) (secondary metabolism). Bacteria typically harbor one PPTase specific for CPs of primary metabolism ("ACPS-type" PPTases) and at least one capable of modifying carrier proteins involved in secondary metabolism ("Sfp-type" PPTases). In order to identify the PPTase(s) associated with erythromycin biosynthesis in Saccharopolyspora erythraea, we have used the genome sequence of this organism to identify, clone, and express (in Escherichia coli) three candidate PPTases: an ACPS-type PPTase (S. erythraea ACPS) and two Sfp-type PPTases (a discrete enzyme (SePptII) and another that is integrated into a modular PKS subunit (SePptI)). In vitro analysis of these recombinant PPTases, with an acyl carrier protein-thioesterase (ACP-TE) didomain from the erythromycin PKS as substrate, revealed that only SePptII is active in phosphopantetheinyl transfer with this substrate. SePptII was also shown to provide complete modification of ACP-TE and of an entire multienzyme subunit from the erythromycin PKS in E. coli. The efficiency of the SePptII in phosphopantetheinyl transfer in E. coli makes it an attractive alternative to other Sfp-type PPTases for co-expression experiments with PKS proteins.     

On the designing of densely dispersion-managed optical fiber systems for ultrafast optical communication

We present some theoretical and experimental results which suggest the possibility of constructing a non-empirical methodology of designing optical transmission systems with ultra high bit-rate per channel. Theoretically, we present an average dispersion decreasing densely dispersion-managed (A4dm) fiber system, which exhibits many advantages over the densely dispersion-managed fiber system, such as the possibility of transmitting chirp-free Gaussian pulses at 160 Gbit/s per channel over transoceanic distances, with a reduced energy and minimal intra-channel interaction. Experimentally we present generation of a 160-GHz picosecond pulse train at 1550 nm using multiple four-wave mixing temporal compression of an initial dual frequency beat signal in the anomalous-dispersion regime of a non-zero dispersion shifted fiber. A complete intensity and phase characterization of the pulse train by means of a frequency-resolved optical gating technique is achieved, showing generation of transform-limited pedestal-free Gaussian pulses.     

Recommendations of RILEM TC 127-MS: Tests for masonry materials and structures

RILEM Technical CommitteesRILEM TC 127-MS: Non Destructive Tests for Masonry Materials and Structures

Recommendations of RILEM TC 127-MS: Tests for masonry materials and structures     

Silicon shadow mask fabrication for patterned metal deposition with microscale dimensions using a novel corner compensation scheme

Pattern deposition of metals with controlled and microscale dimensions can be a challenging task if traditional photolithography is not a practical option. This is a particularly valid concern for the case of certain polymer substrates, which are gaining in importance in the microelectronics and related industries. Therefore, a novel design and process flow for batch fabricating low cost reusable silicon shadow masks was developed. Of note was the corner compensation scheme employed to avoid over-etching of the convex corners in the design. These shadow masks enabled deposition of metals or other suitable materials with feature sizes ranging from approximately 3 to 250 μm and were successfully utilized to form patterned metal heater lines and pads on various samples. The heaters, required for conducting thermal conductivity measurements of the underlying films/substrates using the three omega (3ω) method, showed resistance–temperature linearity, confirming theoretical estimates to within 0.2%. Moreover, the room temperature thermal conductivity of an amorphous SiO₂ film as well as a polyaniline thick film were measured, further validating the deposition through shadow mask technique.     

Application of a new algorithm using Doppler information to retrieve complex wind fields over the Black Sea from ENVISAT SAR images

Several algorithms have been proposed to retrieve near-surface wind fields from C-band synthetic aperture radar (SAR) images acquired over the ocean. They mainly differ in the way they retrieve the wind direction. Conventionally, the wind direction is taken from atmospheric models or is extracted from the linear features sometimes visible in SAR images. Recently, a new wind retrieval algorithm has been proposed, which also includes the Doppler shift induced by motions of the sea surface. In this article, we apply three wind retrieval algorithms, including the one using Doppler information, to three complex wind events encountered over the Black Sea and compare the SAR-derived wind fields with model wind fields calculated using the high-resolution weather research and forecasting (WRF) model. It is shown that the new algorithm is very efficient in resolving the 180° ambiguity in the wind direction, which is often a problem in the streak-based wind retrieval algorithms. However, the Doppler-based algorithm only yields good results for wind directions that have a significant component in the look direction of the SAR antenna. Furthermore, it is dependent on good separation of the contributions to the Doppler shift induced by surface currents and wind-related effects (wind drift and wind-sea components of the ocean wave spectrum). We conclude that an optimum wind retrieval algorithm should consist of a combination of the algorithms based on linear features and Doppler information.     

Generality and legibility in mobile manipulation

This article investigates methods for achieving more general manipulation capabilities for mobile manipulation platforms, which produce legible behavior in human living environments. To achieve generality and legibility, we combine two control mechanisms. First of all, experience- and observation-based learning of skills is applied to routine tasks, so that the repetitive and stereotypical character of everyday activity is exploited. Second, we use planning, reasoning, and search for novel tasks which have no stereotypical solution. We apply these ideas to the learning and use of action-related places, to the model-based visual recognition and localization of objects, and the learning and application of reaching strategies and motions from humans. We demonstrate the integration of these mechanisms into a single low-level control system for autonomous manipulation platforms.     

Bipolar preference modeling and aggregation in decision support

The article discusses the use of positive and negative reasons when preferences about alternative options have to be considered. Besides explaining the intuitive and formal situations where such a bipolar reasoning is used, the article shows how it is possible to generalize the concordance/discordance principle in preference aggregation and apply it to the problem of aggregating preferences expressed under intervals. © 2008 Wiley Periodicals, Inc.     

综合性公园公共交通可达性及规划配置均衡性

公共交通出行方式下,综合性公园的可达性及配置的均衡性是评价城市公园绿地配置和本地居民享有公共服务设施权利的重要指标。以南京市为例,建立公共交通复合网络,以时间成本距离分析综合性公园服务的区域水平和基于站点位置的可达性;以基尼系数判断全局和15分钟生活圈下综合性公园分布的均衡性及贡献度;建立新公园选址采样点,分析增加新公园选址后的基尼系数变化的空间分布。结果表明：以可达性为前提,以基尼系数为衡量指标,可以量化分析公园分布均衡性,通过基尼系数更新变化的空间分布,可以明确新规划公园对综合性公园分布均衡性的影响,能够确定优化选址区域。为城市新综合性公园规划提供量化的区域范围参考,优化城市公园布局并提高城市公园可达性和分布均衡性。     

Soil degradation in the tropical forests of the Dominican Republic's Pedernales province in relation to heavy metal contents

Two of the National Parks of the only Biosphere Reserve in the Caribbean Islands, the Jaragua-Bahoruco-Enriquillo reserve, occur mostly within the Pedernales province (Dominican Republic). In these National Parks, Jaragua and Bahoruco, almost every tropical forest ecosystem is represented, as are the land uses most common to the Dominican Republic. Despite soil being a key natural resource that needs to be considered in any sustainable development programme, the literature contains very little information on the soils of this region. In this study, we analysed 41 topsoil samples representing the main forest types and land uses of the province. The factors examined were fertility (OM, N, P, K) and heavy metal contents (Cu, Cr, Pb, Cd, Ni, Zn). Mean OM, N and total and available metal levels were almost invariably higher in the natural forest soils compared to those given over to human activities, especially cultivated soils. This difference suggests the uptake of metals by the crops and, to a lesser extent, by animals feeding on crop remains and grassland plants. This hypothesis is supported by high total and available metal contents, especially of Zn, Cu, Pb and, in smaller measure, of Cr recorded in the solid waste landfill of the city of Pedernales. It appears that the cutting down and burning practices of a nomad type of cultivation, as well as the pressures of intensive agriculture and livestock rearing have resulted in heavy metals bound to the soil's OM. We propose this is an important point to consider for the management of these lands.     

Spatial heterogeneity of personal exposure to airborne metals in French urban areas

The spatial distribution of urban population exposures to ambient air particles was investigated as part of the Genotox'ER study conducted in four metropolitan areas (Grenoble, Paris, Rouen and Strasbourg) in France. In each city, 60 to 90 non-smoking adult and children volunteers were selected. Subjects lived in three different urban sectors: one highly exposed to traffic emissions, one influenced by local industrial sources, and a background urban environment. The Harvard Chempass multi-pollutant personal sampler was used to sample PM10 and PM2.5 particles during 48 h during two different seasons ('hot' and 'cold'). The elemental composition of the filters was analysed by Particle-Induced X-ray Emission (PIXE). Sixteen elements were found to be over the method detection limits: Al, Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn and Pb. The relative concentrations of elements of crustal origin (Si, Al, Ca) were higher in the coarse fraction of PM10 filters, while elements associated with combustion processes (traffic emissions or industrial combustion) presented higher relative concentrations in the PM2.5 fraction (S, Ni, V, Pb). Spatial heterogeneity of elemental exposures by urban sector is substantial for some metals of health concern, with 20% to 90% greater exposure values, on average, in the traffic proximity or industrial sectors, compared to the background sector, for Fe, Zn, Cu, V and Cr. This spatial heterogeneity should not be overlooked in epidemiological or risk assessment studies.