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排序方式： 共有653条查询结果，搜索用时 15 毫秒
201.
Pavel A. Domnin Vladislav A. Parfenov Alexey S. Kononikhin Stanislav V. Petrov Nataliya V. Shevlyagina Anastasia Yu. Arkhipova Elizaveta V. Koudan Elizaveta K. Nezhurina Alexander G. Brzhozovskiy Anna E. Bugrova Anastasia M. Moysenovich Alexandr A. Levin Pavel A. Karalkin Frederico D. A. S. Pereira Vladimir G. Zhukhovitsky Elena S. Lobakova Vladimir A. Mironov Evgeny N. Nikolaev Yusef D. Khesuani Svetlana A. Ermolaeva 《International journal of molecular sciences》2022,23(3)
Changes in bacterial physiology caused by the combined action of the magnetic force and microgravity were studied in Escherichia coli grown using a specially developed device aboard the International Space Station. The morphology and metabolism of E. coli grown under spaceflight (SF) or combined spaceflight and magnetic force (SF + MF) conditions were compared with ground cultivated bacteria grown under standard (control) or magnetic force (MF) conditions. SF, SF + MF, and MF conditions provided the up-regulation of Ag43 auto-transporter and cell auto-aggregation. The magnetic force caused visible clustering of non-sedimenting bacteria that formed matrix-containing aggregates under SF + MF and MF conditions. Cell auto-aggregation was accompanied by up-regulation of glyoxylate shunt enzymes and Vitamin B12 transporter BtuB. Under SF and SF + MF but not MF conditions nutrition and oxygen limitations were manifested by the down-regulation of glycolysis and TCA enzymes and the up-regulation of methylglyoxal bypass. Bacteria grown under combined SF + MF conditions demonstrated superior up-regulation of enzymes of the methylglyoxal bypass and down-regulation of glycolysis and TCA enzymes compared to SF conditions, suggesting that the magnetic force strengthened the effects of microgravity on the bacterial metabolism. This strengthening appeared to be due to magnetic force-dependent bacterial clustering within a small volume that reinforced the effects of the microgravity-driven absence of convectional flows. 相似文献
202.
Daria D. Petrova Evgeniya V. Dolgova Anastasia S. Proskurina Genrikh S. Ritter Vera S. Ruzanova Yaroslav R. Efremov Ekaterina A. Potter Svetlana S. Kirikovich Evgeniy V. Levites Oleg S. Taranov Alexandr A. Ostanin Elena R. Chernykh Nikolay A. Kolchanov Sergey S. Bogachev 《International journal of molecular sciences》2022,23(24)
An ability of poorly differentiated cells of different genesis, including tumor stem-like cells (TSCs), to internalize extracellular double-stranded DNA (dsDNA) fragments was revealed in our studies. Using the models of Krebs-2 murine ascites carcinoma and EBV-induced human B-cell lymphoma culture, we demonstrated that dsDNA internalization into the cell consists of several mechanistically distinct phases. The primary contact with cell membrane factors is determined by electrostatic interactions. Firm contacts with cell envelope proteins are then formed, followed by internalization into the cell of the complex formed between the factor and the dsDNA probe bound to it. The key binding sites were found to be the heparin-binding domains, which are constituents of various cell surface proteins of TSCs—either the C1q domain, the collagen-binding domain, or domains of positively charged amino acids. These results imply that the interaction between extracellular dsDNA fragments and the cell, as well as their internalization, took place with the involvement of glycocalyx components (proteoglycans/glycoproteins (PGs/GPs) and glycosylphosphatidylinositol-anchored proteins (GPI-APs)) and the system of scavenger receptors (SRs), which are characteristic of TSCs and form functional clusters of cell surface proteins in TSCs. The key provisions of the concept characterizing the principle of organization of the “group-specific” cell surface factors of TSCs of various geneses were formulated. These factors belong to three protein clusters: GPs/PGs, GIP-APs, and SRs. For TSCs of different tumors, these clusters were found to be represented by different members with homotypic functions corresponding to the general function of the cluster to which they belong. 相似文献
203.
Daria Shishkova Arseniy Lobov Bozhana Zainullina Vera Matveeva Victoria Markova Anna Sinitskaya Elena Velikanova Maxim Sinitsky Anastasia Kanonykina Yulia Dyleva Anton Kutikhin 《International journal of molecular sciences》2022,23(23)
Calciprotein particles (CPPs) represent an inherent mineral buffering system responsible for the scavenging of excessive Ca2+ and PO43− ions in order to prevent extraskeletal calcification, although contributing to the development of endothelial dysfunction during the circulation in the bloodstream. Here, we performed label-free proteomic profiling to identify the functional consequences of CPP internalisation by endothelial cells (ECs) and found molecular signatures of significant disturbances in mitochondrial and lysosomal physiology, including oxidative stress, vacuolar acidification, accelerated proteolysis, Ca2+ cytosolic elevation, and mitochondrial outer membrane permeabilisation. Incubation of intact ECs with conditioned medium from CPP-treated ECs caused their pro-inflammatory activation manifested by vascular cell adhesion molecule 1 (VCAM1) and intercellular adhesion molecule 1 (ICAM1) upregulation and elevated release of interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1/ C-C motif ligand 2 (MCP-1/CCL2). Among the blood cells, monocytes were exclusively responsible for CPP internalisation. As compared to the co-incubation of donor blood with CPPs in the flow culture system, intravenous administration of CPPs to Wistar rats caused a considerably higher production of chemokines, indicating the major role of monocytes in CPP-triggered inflammation. Upregulation of sICAM-1 and IL-8 also suggested a notable contribution of endothelial dysfunction to systemic inflammatory response after CPP injections. Collectively, our results demonstrate the pathophysiological significance of CPPs and highlight the need for the development of anti-CPP therapies. 相似文献
204.
Patrizia Frontera Angela Malara Vincenza Modafferi Vincenzo Antonucci Pierluigi Antonucci Anastasia Macario 《加拿大化工杂志》2020,98(9):1924-1934
This work deals with the catalytic performance of nickel-cobalt supported on ceria-doped gadolinia (GDC) catalyst in the single and in the simultaneous methanation of carbon monoxide and carbon dioxide. The catalysts have been prepared by impregnation method, starting from metal salts precursors. Samples have been characterized by x-ray diffraction (XRD), thermogravimetric analysis and differential scanning calorimetry (TGA-DSC), hydrogen temperature programmed reduction (TPR-H2), transmission electronic microscopy (TEM), and scanning electron microscopy (SEM/EDX) technique. The temperature examined for methanation tests ranged from 200°C-600°C. The results show that the prepared and optimized catalysts possess the main characteristics of materials suitable for SOECs (solid oxide electrolyzer cells) applications: high metal content (50% wt/wt with respect to the support), high activity, and high stability. The catalytic performance of bimetallic catalysts highlights that the cobalt does not improve the activity of the nickel catalysts. 相似文献
205.
206.
Natella I. Enukashvily Natalia Semenova Anna V. Chubar Dmitry I. Ostromyshenskii Ekaterina A. Gushcha Sergei Gritsaev Stanislav S. Bessmeltsev Viktor I. Rugal Egor M. Prikhodko Ivan Kostroma Anastasia Zherniakova Anastasia V. Kotova Liubov A. Belik Alexander Shumeev Irina I. Maslennikova Dmitry I. Ivolgin 《International journal of molecular sciences》2022,23(6)
207.
Elena Yu. Kozhunova Galina A. Komarova Oxana V. Vyshivannaya Irina R. Nasimova Anastasia E. Kuvarina Vera S. Sadykova 《International journal of molecular sciences》2022,23(8)
Polymer microgels, including those based on interpenetrating networks (IPNs), are currently vastly studied, and their practical applications are a matter of thriving research. In this work, we show the perspective for the use of polyelectrolyte IPN microgels either as scavengers or carriers of antiseptic substances. Here, we report that poly-N-isopropylacrylamide/polyacrylic acid IPN microgels can efficiently absorb the common bactericidal and virucidal compound benzalkonium chloride. The particles can form a stable aqueous colloidal suspension or be used as building blocks for soft free-standing films. Both materials showed antiseptic efficacy on the examples of Bacillus subtilis and S. aureus, which was approximately equal to the commercial antibiotic. Such polymer biocides can be used as liquid disinfectants, stable surface coatings, or parts of biomedical devices and can enhance the versatility of the possible practical applications of polymer microgels. 相似文献
208.
Anastasia V. Poznyak Evgeny E. Bezsonov Evgeny E. Borisov Andrey V. Grechko Andrey G. Kartuesov Alexander N. Orekhov 《International journal of molecular sciences》2022,23(5)
For the past several decades, humanity has been dealing with HIV. This disease is one of the biggest global health problems. Fortunately, modern antiretroviral therapy allows patients to manage the disease, improving their quality of life and their life expectancy. In addition, the use of these drugs makes it possible to reduce the risk of transmission of the virus to almost zero. Atherosclerosis is another serious pathology that leads to severe health problems, including disability and, often, the death of the patient. An effective treatment for atherosclerosis has not yet been developed. Both types of immune response, innate and adaptive, are important components of the pathogenesis of this disease. In this regard, the peculiarities of the development of atherosclerosis in HIV carriers are of particular scientific interest. In this review, we have tried to summarize the data on atherosclerosis and its development in HIV carriers. We also looked at the classic therapeutic methods and their features concerning the concomitant diagnosis. 相似文献
209.
Alena Y. Drobiazko Anastasia A. Kasimova Peter V. Evseev Mikhail M. Shneider Evgeniy I. Klimuk Alexander S. Shashkov Andrei S. Dmitrenok Alexander O. Chizhov Pavel V. Slukin Yuriy P. Skryabin Nikolay V. Volozhantsev Konstantin A. Miroshnikov Yuriy A. Knirel Anastasia V. Popova 《International journal of molecular sciences》2022,23(9)
In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types. 相似文献
210.
Alexey Orlov Savva Semenov Gleb Rukhovich Anastasia Sarycheva Oxana Kovaleva Alexander Semenov Elena Ermakova Ekaterina Gubareva Anna E. Bugrova Alexey Kononikhin Elena I. Fedoros Evgeny Nikolaev Alexander Zherebker 《International journal of molecular sciences》2022,23(24)
Chronic liver diseases affect more than 1 billion people worldwide and represent one of the main public health issues. Nonalcoholic fatty liver disease (NAFLD) accounts for the majority of mortal cases, while there is no currently approved therapeutics for its treatment. One of the prospective approaches to NAFLD therapy is to use a mixture of natural compounds. They showed effectiveness in alleviating NAFLD-related conditions including steatosis, fibrosis, etc. However, understanding the mechanism of action of such mixtures is important for their rational application. In this work, we propose a new dereplication workflow for deciphering the mechanism of action of the lignin-derived natural compound mixture. The workflow combines the analysis of molecular components with high-resolution mass spectrometry, selective chemical tagging and deuterium labeling, liver tissue penetration examination, assessment of biological activity in vitro, and computational chemistry tools used to generate putative structural candidates. Molecular docking was used to propose the potential mechanism of action of these structures, which was assessed by a proteomic experiment. 相似文献