Self-assembling of star-like amphiphilic block copolymers with polyelectrolyte blocks. Effect of pH

The self-assembling behaviour of a four-arm amphiphilic star block copolymer, (PMMA₇₃-b-PAA₁₄₃)₄, with poly(methyl methacrylate) inner blocks and poly(acrylic acid) outer blocks in ratio 1:2 (PMMA:PAA) has been investigated in aqueous solutions as a function of pH by dynamic light scattering and cryo-transmission electron microscopy. At low pH (pH ≤ 5) the amphiphile forms in the presence of salt both spherical and worm-like micellar aggregates that coexist in solution. At high pH (pH > 12) the solution contains mainly spherical micelles and a small number of larger aggregates that have ‘pearl-necklace’ structure, indicating the disintegration of the worm-like species. In addition to the experiments, computer simulations of the four-arm amphiphilic star block copolymer with the same ratio of the blocks as above were conducted using a coarse-grained model. The simulations predict the formation of the worm-like micellar aggregates at low pH and the spherical ones at high pH. The changes in the morphology of the aggregates are related to the higher degree of ionization of poly(acrylic acid) blocks at high pH and to the swelling of the corona of the micelles by the higher osmotic pressure due to trapped counterions.     

Polymerized high internal phase emulsions containing a porogen: Specific surface area and sorption

Highly porous, open‐cell polymers [poly(HIPE)] were prepared by polymerizing the monomers in the continuous phase (～ 10%) of high internal phase emulsions (HIPE). This paper discusses using poly(HIPE) to remove bromoform from an aqueous solution through sorption, a combination of adsorption and absorption. The crosslinked polystyrene (xPS) and crosslinked poly(ethylhexyl acrylate) (xPEHA) had cell diameters from 1.5 to 15 μm, intercellular pore diameters from 0.3 to 1.5 μm, and densities of about 0.10 g/cc. The specific surface area of the glassy xPS increased from 7.9 to 28.8 m²/g on extraction in methanol, most likely due to crazing. The use of a toluene porogen in the xPS (xPS‐T) reduced the density to 0.05 g/cc and yielded a rough surface with nanoscale porosity and a specific surface area of 132 m²/g. xPS and xPEHA, with very different molecular structures but with similar specific surface areas, exhibited similar sorption behavior. Extraction produced increases in the xPS and xPS‐T sorption plateaus and sorption capacities. For larger specific surface areas, the sorption at low concentrations was relatively independent of concentration, indicating a case of adsorption with the sites occupied. For all the other cases, absorption seems to dominate and sorption is more strongly dependent upon concentration. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 2233–2239, 2004     

Cadmium Chalcogenide Nano‐Heteroplatelets: Creating Advanced Nanostructured Materials by Shell Growth,Substitution, and Attachment

The current direction in the evolution of 2D semiconductor nanocrystals involves the combination of metal and semiconductor components to form new nanoengineered materials called nano‐heteroplatelets. This Review covers different heterostructure architectures that can be applied to cadmium chalcogenide nanoplatelets, including variously shaped shell, metal nanoparticle decoration, and doped and alloy systems. Here, for the first time a complete classification of nano‐heteroplatelet types is provided with recommended notations and a systematization of the existing knowledge and experience concerning heterostructure formation techniques, addressing the morphology, optoelectronic and magnetic properties, and novel features of different heterostructures. This Review is also devoted to possible applications of these heterostructures and of one‐component nanoplatelets in multiple fields, including light‐emitting devices and biological imaging.     

A fracture locus for a 50 volume-percent Al/SiC metal matrix composite at high temperature

The effect of the stress state on the fracture locus function of the 50 vol.% Al/SiC metal matrix composite at high temperature is studied. The value of fracture locus function is quantitatively characterized by the amount of shear strain accumulated prior to the moment of failure. Nondimensional invariant parameters are used as characteristics of the stress state, namely, the stress triaxiality k and the Lode-Nadai coefficient μ _σ showing the form of the stress state. Besides conventional testing for tension, compression and torsion of smooth cylindrical specimens, the complex of mechanical tests includes a new type of testing, namely, that for bell-shaped specimens. These kinds of testing enable one to study fracture strain under monotonic deformation in the ranges μ _σ ?=?0?…?+?1 and k?=???1.08...0 without using high-pressure technologies. The stress–strain state during specimen testing is here evaluated from the finite element simulation of testing in ANSYS. The tests were performed at a temperature of 300 °C and shear strain rate intensity Η?=?0.1;?0.3;?0.5 1/s. The test results have offered a fracture locus, which can be used in models of damage mechanics to predict fracture of the material in die forging processes.     

Electrochemical assessment of the interaction of microbial living cells and carbon nanomaterials

This work considers the effects of various carbon nanomaterials and fibres on bioelectrocatalytic and respiratory activity of bacterial cells during the oxidation of ethanol in the presence of an electron transport mediator. Gluconobacter oxydans sbsp. industrius VKM B‐1280 cells were immobilised on the surfaces of graphite electrodes and had an adsorption contact with a nanomaterial (multi‐walled carbon nanotubes, thermally expanded graphite, highly oriented pyrolytic graphite, graphene oxide, reduced graphene oxide). The electrochemical parameters of the electrodes (the polarisation curves, the value of generated current at the introduction of substrate, the impedance characteristics) were measured in two‐electrode configuration. Modification by multi‐walled carbon nanotubes led to the increase of microbial fuel cell (MFC) electric power by 26%. The charge transfer resistance of modified electrodes was 47% lower than unmodified ones. Thermally expanded and pyrolytic graphites had a slight negative effect on the electrochemical properties of modified electrodes. The respiratory activity of bacterial cells did not change in the presence of nanomaterials. The data can be used in the development of microbial biosensors and MFC electrodes based on Gluconobacter cells.Inspec keywords: nanofabrication, catalysis, microorganisms, adsorption, charge exchange, microbial fuel cells, electrochemical electrodes, graphite, graphene, oxidation, multi‐wall carbon nanotubes, cellular biophysicsOther keywords: reduced graphene oxide, electrochemical parameters, two‐electrode configuration, multiwalled carbon nanotubes, microbial fuel cell, respiratory activity, bacterial cells, microbial biosensors, MFC electrodes, microbial living cells, electron transport mediator, graphite electrodes, adsorption contact, highly oriented pyrolytic graphite, Gluconobacter oxydans sbsp. industrius VKM B‐1280 cells, polarisation curves, bioelectrocatalytic activity, ethanol, thermally expanded graphite, charge transfer resistance, C     

A Confocal Microscopic Study of Gene Transfer into the Mesencephalic Tegmentum of Juvenile Chum Salmon,Oncorhynchus keta,Using Mouse Adeno-Associated Viral Vectors

To date, data on the presence of adenoviral receptors in fish are very limited. In the present work, we used mouse recombinant adeno-associated viral vectors (rAAV) with a calcium indicator of the latest generation GCaMP6m that are usually applied for the dorsal hippocampus of mice but were not previously used for gene delivery into fish brain. The aim of our work was to study the feasibility of transduction of rAAV in the mouse hippocampus into brain cells of juvenile chum salmon and subsequent determination of the phenotype of rAAV-labeled cells by confocal laser scanning microscopy (CLSM). Delivery of the gene in vivo was carried out by intracranial injection of a GCaMP6m-GFP-containing vector directly into the mesencephalic tegmentum region of juvenile (one-year-old) chum salmon, Oncorhynchus keta. AAV incorporation into brain cells of the juvenile chum salmon was assessed at 1 week after a single injection of the vector. AAV expression in various areas of the thalamus, pretectum, posterior-tuberal region, postcommissural region, medial and lateral regions of the tegmentum, and mesencephalic reticular formation of juvenile O. keta was evaluated using CLSM followed by immunohistochemical analysis of the localization of the neuron-specific calcium binding protein HuCD in combination with nuclear staining with DAPI. The results of the analysis showed partial colocalization of cells expressing GCaMP6m-GFP with red fluorescent HuCD protein. Thus, cells of the thalamus, posterior tuberal region, mesencephalic tegmentum, cells of the accessory visual system, mesencephalic reticular formation, hypothalamus, and postcommissural region of the mesencephalon of juvenile chum salmon expressing GCaMP6m-GFP were attributed to the neuron-specific line of chum salmon brain cells, which indicates the ability of hippocampal mammal rAAV to integrate into neurons of the central nervous system of fish with subsequent expression of viral proteins, which obviously indicates the neuronal expression of a mammalian adenoviral receptor homolog by juvenile chum salmon neurons.     

Cascade subcritical liquid-salt reactor for burning transplutonium actinides

A cascade subcritical liquid-salt reactor designed for burning long-lived components of the radioactive wastes of the nuclear fuel cycle is examined. The cascade scheme of the reactor makes it possible to decrease by a factor of three the power of the driving accelerator as compared with conventional accelerator-blanket systems of equal power. The fuel composition of the reactor consists of 20% Np, Am, Cm, and other transplutonium elements and 80% plutonium, which are dissolved in a salt melt NaF(50%)-ZrF₄(50%). For a 10 MW proton accelerator, 1 GeV proton energy (10 mA current) and subcriticality depth 0.05, the thermal power of the reactor is 800 MW, which permits burning ∼70 kg/yr Np, Am, Cm, and other transplutonium actinides, i.e., service five VVéR type reactors of equal power. __________ Translated from Atomnaya énergiya, Vol. 101, No. 2, pp. 116–125, August, 2006.     

Microstructure Control of In-Situ-Toughened α-SiAlON Ceramics

Single-phase α-SiAlON with elongated grains is obtained from α-Si₃N₄ powder for a broad range of compositions of practical interest. Following the concept of nucleation and growth, two-step firing is used for microstructure control. This method takes advantage of the slow transformation reaction from α-Si₃N₄ to α-SiAlON at low temperature when the composition is near the α-SiAlON phase boundary and, hence, is marginally stable. For more-stable compositions, the seeding of α-SiAlON crystals is more effective, because it allows elongated grains to grow onto the seed crystals. The fracture toughness is strongly correlated with the microstructure and is enhanced greatly in the optimized materials.