Glycosidic linkages of legume,grass and cereal straw cell walls before and after extensive degradation by rumen microorganisms

The technique of methylation analysis for the location of glycosidic linkages in cell wall polysaccharides was applied directly (after dry milling in liquid nitrogen) to whole wheat and barley straws, cell wall preparations from early- and late-cut perennial ryegrass and white clover, and their extensively degraded residues recovered from the rumen. The addition of an internal standard, methyl-β-D-allo-pyranose, to milled samples enabled the recovery of partially-methylated sugars to be quantified, and the recovery of parent sugars from methylated material to be compared with values obtained by direct estimation of monosaccharides by the alditol acetate method. Recovery of sugars from methylated samples was generally higher than that predicted by the alditol acetate method, particularly from digested residues. All four samples of Gramineae showed similar proportions of glycosidic linkages in which (1 →4)-linked xylose and glucose units accounted for more than 80% of total identified partially-methylated sugars. The proportions of glycosidic linkages found in digested residues of straws were essentially the same as those found in the parent material. In residues of the more digestible ryegrass there was a two- to three-fold increase in (1→4)-linked xylose units without branch points at the O-2/O-3 positions, the proportions of these branch points being substantially reduced. A corresponding reduction in the proportion of (1→2)-, (1→3)-and terminally-linked arabinose units was also found. The proportion of both (1→4)- and (1→3)-linked glucose units were also reduced in digested residues. These changes were greater in the early-cut than in the late-cut sample. White clover differed from the Gramineae in a number of respects. Galactose units predominantly in the form of (1→4)-, (1→6)- and terminally-linked units, were the major non-cellulosic sugars present. The proportion of all forms of galactose was reduced in digested residues, but (1→6)-linked units appeared more resistant to degradation than (1→4)-linked units. Like ryegrass, the proportion of xylose units increased in digested residues from clover, while the proportion of glucose decreased. The proportion of (1→4)-linked mannose units, initially present at levels ten times as great as in the Gramineae, was also substantially reduced.     

Treatment of milk by gamma irradiation—effect of anoxia on lipid peroxidation and the survival of Pseudomonas aeruginosa

One of the causes of organoleptic changes in milk, lipid peroxidation, has been monitored by a sensitive assay newly applied to foodstuffs. It has been shown that even at medium radiation doses (up to 3600 Gy) negligible lipid peroxidation takes place, providing the milk is thoroughly gassed with nitrogen. Using a test spoilage microorganism, Pseudomonas aeruginosa (Schroeter), it was shown that the D₁₀ was 35 Gy in air and 105 Gy under anaerobic conditions. Dose/survival curves indicate that 5 decades of killing needed 200 Gy in air and 600 Gy under nitrogen. However, even after receiving a dose of 1600 Gy, both aerobically and anaerobically treated milk failed the methylene blue test at 21 days post-irradiation. The data indicate that, although the deleterious lipid peroxidation effects of irradiation can be minimised by the removal of air, this in turn results in a degree of protection being afforded to the bacteria, and that doses in excess of 1600 Gy are needed to effectively sterilise milk.     

Extracellular recordings from patterned neuronal networks using planar microelectrode arrays

Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (microCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-microm-wide lines spaced by 50-200 microm with 15-25-microm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a approximately 2 microm bundle of guided neurites.     

Ochratoxin A in dried vine fruit: method development and survey

A method is described for the determination of concentrations of the mycotoxin ochratoxin A in dried vine fruits (currants, raisins and sultanas) using acidic methanolic extraction,immunoaffinity chromatography clean-up and HPLC determination. The limit of detection was estimated as 0.2mug/kg, and recoveries of 63-77% were achieved at 5mug/kg. HPLC-mass spectrometric confirmation of the identity of ochratoxin was obtained. Ochratoxin A and aflatoxins were determined in 60 samples of retail dried vine fruits purchased in the United Kingdom. Ochratoxin A was found in excess of 0.2mug/kg in 19 of 20 currant, 17 of 20 sultana and 17 of 20 raisin samples examined, an overall incidence of 88% . The maximum level found was 53.6mug/kg. No aflatoxin was found in any sample analysed, using a method with a detection limit of 0.2mug/kg for each of aflatoxin B1, B2, G1 and G2.     

Consideration of chiral optical fibres

Circular birefringence is a property of chiral materials. In this work, we consider the use of chiral materials in optical fibres to produce circularly birefringent optical fibres and in fibres where a contrast in circular birefringence contributes to forming the waveguide. (-)-menthyl methacrylate is also investigated as a possible material for the fabrication of such fibres.     

Recovering missing slices of the discrete fourier transform using ghosts

The discrete Fourier transform (DFT) underpins the solution to many inverse problems commonly possessing missing or unmeasured frequency information. This incomplete coverage of the Fourier space always produces systematic artifacts called Ghosts. In this paper, a fast and exact method for deconvolving cyclic artifacts caused by missing slices of the DFT using redundant image regions is presented. The slices discussed here originate from the exact partitioning of the Discrete Fourier Transform (DFT) space, under the projective Discrete Radon Transform, called the discrete Fourier slice theorem. The method has a computational complexity of O(nlog(2)n) (for an n=N×N image) and is constructed from a new cyclic theory of Ghosts. This theory is also shown to unify several aspects of work done on Ghosts over the past three decades. This paper concludes with an application to fast, exact, non-iterative image reconstruction from a highly asymmetric set of rational angle projections that give rise to sets of sparse slices within the DFT.     

Coding gain and spatial localisation properties of discrete wavelettransform filters for image coding

The authors consider coding gain and spatial localisation properties of DWT filters for still image compression. Using a JPEG type quantisation and encoding method several images are compressed using a DWT implemented using various two-band subband filter sets. It is concluded that a relatively high coding gain (relative to a highly correlated source) is necessary, but not sufficient, for good image coding performance. Further, it is observed that low spatial width filters are desirable, particularly in regard to reduced ringing distortion. In terms of the trade-off between coding gain and spatial localisation, and in terms of actual coding performance, it is shown that biorthogonal filters can outperform orthogonal filters     

Energy-efficient Hardware Accelerators for the SA-DCT and Its Inverse

The explosive growth of the mobile multimedia industry has accentuated the need for efficient VLSI implementations of the associated computationally demanding signal processing algorithms. In particular, the short battery life caused by excessive power consumption of mobile devices has become the biggest obstacle facing truly mobile multimedia. We propose novel hardware accelerator architectures for two of the most computationally demanding algorithms of the MPEG-4 video compression standard––the forward and inverse shape adaptive discrete cosine transforms (SA-DCT/IDCT). These accelerators have been designed using general low-energy design philosophies at the algorithmic/architectural abstraction levels. The themes of these philosophies are avoiding waste and trading area/performance for power and energy gains. Each core has been synthesised targeting TSMC 0.09 μm TCBN90LP technology, and the experimental results presented in this paper show that the proposed cores improve upon the prior art.

Improved reconstruction for MR spectroscopic imaging

Sensitivity limitations of in vivo magnetic resonance spectroscopic imaging (MRSI) require that the extent of spatial-frequency (k-space) sampling be limited, thereby reducing spatial resolution and increasing the effects of Gibbs ringing that is associated with the use of Fourier transform reconstruction. Additional problems occur in the spectral dimension, where quantitation of individual spectral components is made more difficult by the typically low signal-to-noise ratios, variable lineshapes, and baseline distortions, particularly in areas of significant magnetic field inhomogeneity. Given the potential of in vivo MRSI measurements for a number of clinical and biomedical research applications, there is considerable interest in improving the quality of the metabolite image reconstructions. In this report, a reconstruction method is described that makes use of parametric modeling and MRI-derived tissue distribution functions to enhance the MRSI spatial reconstruction. Additional preprocessing steps are also proposed to avoid difficulties associated with image regions containing spectra of inadequate quality, which are commonly present in the in vivo MRSI data     

Investigation of hot carrier degradation in asymmetric nDeMOS transistors

Hot carrier degradation in asymmetric nDeMOS transistors is investigated in this paper. It is found that the worst case hot carrier stress condition in asymmetric nDeMOS transistors is at I_g,max, and not at I_b,max and hot-electron injection (HE, i.e. V_gs = V_ds). Further, the damage regions in transistors upon various hot carrier stress modes are located by using variable amplitude charge pumping technique. It is found that the interface traps generation in the gate/n-type graded drain (NGRD) overlap and spacer/NGRD regions is the dominant mechanism of hot carrier degradation in transistors upon I_g,max stress mode. Moreover, both the interface trap generation and the electron trapping are two important factors to induce the electrical parameters shifts of asymmetric nDeMOS transistors under I_b,max and HE stress.