The stability and unfolding of an IgG binding protein based upon the B domain of protein A from Staphylococcus aureus probed by tryptophan substitution and fluorescence spectroscopy

The stability and unfolding of an immunoglobulin (Ig) G bindingprotein based upon the B domain of protein A (SpAB) from Staphylococcusaureus were studied by substituting tryptophan residues at strategiclocations within each of the three a-helical regions (al-a3)of the domain. The role of the C-terminal helix, a3, was investigatedby generating two protein constructs, one corresponding to thecomplete SpAB, the other lacking a part of ct3; the Trp substitutionswere made in both one-and two-domain versions of each of theseconstructs. The fluorescence properties of each of the single-tryptophanmutants were studied in the native state and as a function ofguanidine-HCl-mediated unfolding, and their IgG binding activitieswere determined by a competitive enzyme-linked immunosorbentassay. The free energies of folding and of binding to IgG foreach mutant were compared with those for the native domains.The effect of each substitution upon the overall structure andupon the IgG binding interface was modelled by molecular graphicsand energy minimization. These studies indicate that (i) 3 contributesto the overall stability of the domain and to the formationof the IgG binding site in l and 2, and (ii) al unfolds first,followed by 2 and 3 together.     

Activated gas plasma surface treatment of polymers for adhesive bonding

Polyethylene, polypropylene, poly(vinyl fluoride) (Tedlar), polystyrene, nylon 6, poly(ethylene terephthalate) (Mylar), polycarbonate, cellulose acetate butyrate, and a poly(oxymethylene) copolymer were treated with activated helium and with activated oxygen. Mechanical strengths of adhesive-bonded specimens prepared from treated and from untreated coupons were compared. Polyethylene (PE) and polypropylene (PP) showed the greatest increases in bond strength. Oxygen and helium were both effective with polyethylene, but polypropylene showed no improvement when treated with activated helium. The results with excited helium parallel the effects of ionizing radiation on these two polymers, as does the appearance of unsaturation bands in the infrared (965 cm^?1 in PE, and 887 and 910 cm^?1 in PP). Active nitrogen produced excellent bond strength with polyethylene but not with polypropylene. Of the remaining polymers examined, Tedlar, polystyrene, and nylon 6 showed the greatest improvement in bondability after treatment, and Mylar showed moderate improvement. Polycarbonate, cellulose acetate butyrate, and the poly(oxymethylene) copolymer gave approximately two-fold increases in lap-shear bond strength. In several cases, significant differences in response to time of treatment and type of excited gas were found.     

Prevention of water pollution problems in mining: The bactericide technology

In pyritic environments, the bacteriaThiobacillus ferrooxidans catalyze acid formation by increasing the oxidation rate of pyrite by a factor of one million. This acid solubilizes metals and pollutes adjacent streams and lands. Bactericide sprays during mining and waste disposal operations attack the source of the problem by preventing acid formation and metals solubilization. Used in conjunction with current water treatment systems, bactericides can dramatically reduce operating costs. Controlled release bactericides contribute to successful reclamation by providing assurance against revegetation failure and post-reclamation water quality problems that can necessitate perpetual water treatment. While inhibitingT. ferrooxidans these organic compounds aid in the establishment of beneficial heterotrophic bacteria which support vegetation. These conditions continue to persist after the bactericide is depleted from the controlled release system. Case Studies I and II show that bactericides inhibit acid generation during hard rock and coal mining operations and they are cost effective. Case Studies III and IV illustrate the improvement in water quality and vegetation after reclamation when controlled release bactericides were used. Economic analyses show cost benefits are achieved when controlled-release bactericides are part of the reclamation plan.     

Boron distribution in a low-alloy steel

Boron distribution in a low-alloy steel (15B26:0.25C-0.29Cr-0.03Ti-0.028Al-0.0016B) has been characterized employing Fission Track Etching (FTE) method. The characteristics of boron distribution with variation of cooling rate after austenitization and through case-hardened depth after carburization were analyzed. Hardenability of 15B26 steel was also evaluated through Jominy-end-quench test and the results are as follows: It was observed that, in austenitized 15B26 steel, boron was distributed uniformly over the whole area of specimen with a little segregation along the austenite grain boundaries at higher cooling rates and boron precipitates were formed in the intergranular as well as transgranular regions at lower cooling rates. Jominy equivalents (HRC 35) of 15B26 steel were fairly increased between the Jominy temperatures of 820°C and 850°C, which might result from the increase of the amount of soluble boron in austenite due to the dissolution of borocarbides between 820°C and 850°C. In carburized 15B26 steel, the different through thickness features of boron distribution from the carburized surface were found; coarse nodular boron precipitates up to the depth of 150 μm; uniform distribution of dissolved boron between 150~650 μm; and segregation of boron atoms along grain boundaries in the regions deeper than 650 μm.     

The significance of deposition point standoff variations in multiple-layer coaxial laser cladding (coaxial cladding standoff effects)

Direct laser deposition (DLD) is an additive manufacturing process that builds up a part layer-by-layer by fusing metal powder to a solid substrate. A coaxial laser and powder delivery head is commonly used, and here a critical process variable is the standoff distance between the nozzle and the material deposition point. This contribution investigates the role of this variable and the effect that it has on the final part geometry. The experimental results show that good layer consistency can, in fact, be obtained with no movement of the substrate between layers for more than 20 layers, or 10 mm under tested conditions, and that poorer results are obtained by regular movement of the substrate by a badly chosen constant amount. A theoretical analysis of the situation establishes a method to estimate melt pool size using an analytically calculated temperature near the heat source. Based on this, simple heat and mass flow models are established to allow some of the results to be explained.     

Object tracking based on an online learning network with total error rate minimization

This paper presents a visual object tracking system which is tolerant to external imaging factors such as illumination, scale, rotation, occlusion and background changes. Specifically, an integration of an online version of total-error-rate minimization based projection network with an observation model of particle filter is proposed to effectively distinguish between the target object and the background. A re-weighting technique is proposed to stabilize the sampling of particle filter for stochastic propagation. For self-adaptation, an automatic updating scheme and extraction of training samples are proposed to adjust to system changes online. Our qualitative and quantitative experiments on 16 public video sequences show convincing performances in terms of tracking accuracy and computational efficiency over competing state-of-the-art algorithms.     

PalmPhasor算法性能的理论分析

模板的安全性和隐私性是掌纹系统实际应用的关键问题,然而生物特征保护的多项指标通常相互冲突并且难以同时满足.作为解决上述冲突的一种可撤销掌纹编码算法,PalmPhasor实现了高效、安全的掌纹认证.建立了系统分析PalmPhasor性能的完整框架.为了便于具体分析,将情景分为4种情况,并且提供了支持相应分析的预备知识,包括辅助定理以及Gabor滤波掌纹图像实部和虚部分布特性.在统计学基础上建立的理论分析和实验结果均表明:即使在用户口令被盗的情况下,多方向分数级融合增强的PalmPhasor算法也可以同时有效地满足可撤销生物特征的4项指标.     

Privacy Preserving Geo-Linkage in the Big Urban Data Era

Big data technologies and a range of Government open data initiatives provide the basis for discovering new insights into cities; how they are planned, how they managed and the day-to-day challenges they face in health, transport and changing population profiles. The Australian Urban Research Infrastructure Network (AURIN – www.aurin.org.au) project is one example of such a big data initiative that is currently running across Australia. AURIN provides a single gateway providing online (live) programmatic access to over 2000 data sets from over 70 major and typically definitive data-driven organizations across federal and State government, across industry and across academia. However whilst open (public) data is useful to bring data-driven intelligence to cities, more often than not, it is the data that is not-publicly accessible that is essential to understand city challenges and needs. Such sensitive (unit-level) data has unique requirements on access and usage to meet the privacy and confidentiality demands of the associated organizations. In this paper we highlight a novel geo-privacy supporting solution implemented as part of the AURIN project that provides seamless and secure access to individual (unit-level) data from the Department of Health in Victoria. We illustrate this solution across a range of typical city challenges in localized contexts around Melbourne. We show how unit level data can be combined with other data in a privacy-protecting manner. Unlike other secure data access and usage solutions that have been developed/deployed, the AURIN solution allows any researcher to access and use the data in a manner that meets all of the associated privacy and confidentiality concerns, without obliging them to obtain ethical approval or any other hurdles that are normally put in place on access to and use of sensitive data. This provides a paradigm shift in secure access to sensitive data with geospatial content.     

Size-separable tile self-assembly: a tight bound for temperature-1 mismatch-free systems

We introduce a new property of tile self-assembly systems that we call size-separability. A system is size-separable if every terminal assembly is a constant factor larger than any intermediate assembly. Size-separability is motivated by the practical problem of filtering completed assemblies from a variety of incomplete “garbage” assemblies using gel electrophoresis or other mass-based filtering techniques. Here we prove that any system without cooperative bonding assembling a unique mismatch-free terminal assembly can be used to construct a size-separable system uniquely assembling the same shape. The proof achieves optimal scale factor, temperature, and tile types (within a factor of 2) for the size-separable system.     

Matrix-free aerostructural optimization of aircraft wings

In structural optimization subject to failure constraints, computing the gradients of a large number of functions with respect to a large number of design variables may not be computationally practical. Often, the number of constraints in these optimization problems is reduced using constraint aggregation at the expense of a higher mass of the optimal structural design. This work presents results of structural and coupled aerodynamic and structural design optimization of aircraft wings using a novel matrix-free augmented Lagrangian optimizer. By using a matrix-free optimizer, the computation of the full constraint Jacobian at each iteration is replaced by the computation of a small number of Jacobian-vector products. The low cost of the Jacobian-vector products allows optimization problems with thousands of failure constraints to be solved directly, mitigating the effects of constraint aggregation. The results indicate that the matrix-free optimizer reduces the computational work of solving the optimization problem by an order of magnitude compared to a traditional sequential quadratic programming optimizer. Furthermore, the use of a matrix-free optimizer makes the solution of large multidisciplinary design problems, in which gradient information must be obtained through iterative methods, computationally tractable.