Dynamic mechanical measurements: Comparison between bending and torsion methods on a graphite-reinforced and a rubber-modified epoxy

Dynamic mechanical measurements in the cantilever bending and torsion rectangular modes were compared on two epoxy samples: one unidirectionally reinforced by 42 vol.% graphite fibers, the other modified by 15 wt.% carboxy terminated acrylonitrile butadiene rubber. Two forced oscillation instruments were used: cantilever bending on a Polymer Labs DMTA and torsion with a Rheometrics System Four. In the cantilever mode, the storage modulus on both samples was measured at about two to three times too low because of compliance of the instrument. The two instruments agreed and appeared to measure accurately tan δ on the graphite-reinforced samples. Agreement was only fair on tan δ for the rubber-modified sample. Multiple frequency data were used to separate the glass-transition temperature of the rubber from the epoxy β transition. Activation energies of these transitions are within the range reported. This study demonstrates the value of forced oscillation methods and the importance of selecting sample dimensions to fit instrument limits.     

Ruthenium(II)–Arene Thiocarboxylates: Identification of a Stable Dimer Selectively Cytotoxic to Invasive Breast Cancer Cells

Ru^II-arene complexes provide a versatile scaffold for novel anticancer drugs. Seven new Ru^II-arene-thiocarboxylato dimers were synthesized and characterized. Three of the complexes ( 2 a , b and 5 ) showed promising antiproliferative activities in MDA-MB-231 (human invasive breast cancer) cells, and were further tested in a panel of fifteen cancerous and noncancerous cell lines. Complex 5 showed moderate but remarkably selective activity in MDA-MB-231 cells (IC₅₀=39±4 μm Ru). Real-time proliferation studies showed that 5 induced apoptosis in MDA-MB-231 cells but had no effect in A549 (human lung cancer, epithelial) cells. By contrast, 2 a and b showed moderate antiproliferative activity, but no apoptosis, in either cell line. Selective cytotoxicity of 5 in aggressive, mesenchymal-like MDA-MB-231 cells over many common epithelial cancer cell lines (including noninvasive breast cancer MCF-7) makes it an attractive lead compound for the development of specifically antimetastatic Ru complexes with low systemic toxicity.     

新型羊毛阻燃剂

提供了传统的羊毛阻燃剂Zirpro和自由Firestop化学品公司开发的新的替代产品Naflan深入的技术分析结果.描述了Zirpro处理和Norflan处理的优缺点.讨论了Noflan多用途整理.     

Asymptotics for stationary very nearly unit root processes

Abstract. This article considers a mean zero stationary first‐order autoregressive (AR) model. It is shown that the least squares estimator and t statistic have Cauchy and standard normal asymptotic distributions, respectively, when the AR parameter ρ_n is very near to one in the sense that 1 ? ρ_n = o(n^?1).     

Effects of substitution of Thr63 by alanine on the structure and function of Lactobacillus casei dihydrofolate reductase

A mutant of Lactobacillus casei dihydrofolate reductase hasbeen constructed in which Thr63, a residue which interacts withthe 2'-phosphate group of the bound coenzyme, is replaced byalanine. This substitution does not affect k_cat, but producesan 800-fold increase in the K_m for NADPH, which reflects dissociationof NADPH from the enzyme-NADPH-tetrahydrofolate complex, anda 625-fold increase (corresponding to 3.8 kcal/mol) in the dissociationconstant for the enzyme-NADPH complex. The difference in magnitudeof these effects indicates a small effect of the substitutionon the negative cooperativity between NADPH and tetrahydrofolate.Stopped-flow studies of the kinetics of NADPH binding show thatthe weaker binding arises predominantly from a decrease in theassociation rate constant. NMR spectroscopy was used to comparethe structures of the mutant and wild-type enzymes in solution,in their complexes with methotrexate and with methotrexate andNADPH. This showed that only minimal structural changes resultfrom the mutation; a total of 47 residues were monitored fromtheir resolved ¹H resonances, and of these nine in the binarycomplex and six in the ternary differed in chemical shift betweenmutant and wild-type enzyme. These affected residues are confinedto the immediate vicinity of residue 63. There is a substantialdifference in the ³¹P chemical shift of the 2'-phosphate ofthe bound coenzyme, reflecting the loss of the interaction withthe side chain of Thr63. The only changes in nuclear Overhausereffects (NOEs) observed were decreases in the intensity of NOEsbetween protons of the adenine ring of the bound coenzyme andthe nearby residues Leu62 and Ile102, showing that the substitutionof Thr63 does cause a change in the position or orientationof the adenine ring in its binding site.     

REPLACE: a strategy for iterative design of cyclin-binding groove inhibitors

We describe a drug-design strategy termed REPLACE (REplacement with Partial Ligand Alternatives through Computational Enrichment) in which nonpeptidic surrogates for specific determinants of known peptide ligands are identified in silico by using a core peptide-bound protein structure as a design anchor. In the REPLACE application example, we present the effective replacement of two critical binding motifs in a lead protein-protein interaction inhibitor pentapeptide with more druglike phenyltriazole and diphenyl ether groups. These were identified through docking of fragment libraries into the volume of the cyclin-binding groove of CDK2/cyclin A vacated through truncation of the inhibitor peptide-binding determinants. Proof of concept for this strategy was obtained through the generation of potent peptide-small-molecule hybrids and by the confirmation of inhibitor-binding modes in X-ray crystal structures. This method therefore allows nonpeptide fragments to be identified without the requirement for a high-sensitivity binding assay and should be generally applicable in replacing amino acids as individual residues or groups in peptide inhibitors to generate pharmaceutically acceptable lead molecules.     

Stochastic Geometry and Random Graphs for the Analysis and Design of Wireless Networks

Wireless networks are fundamentally limited by the intensity of the received signals and by their interference. Since both of these quantities depend on the spatial location of the nodes, mathematical techniques have been developed in the last decade to provide communication-theoretic results accounting for the network?s geometrical configuration. Often, the location of the nodes in the network can be modeled as random, following for example a Poisson point process. In this case, different techniques based on stochastic geometry and the theory of random geometric graphs ? including point process theory, percolation theory, and probabilistic combinatorics ? have led to results on the connectivity, the capacity, the outage probability, and other fundamental limits of wireless networks. This tutorial article surveys some of these techniques, discusses their application to model wireless networks, and presents some of the main results that have appeared in the literature. It also serves as an introduction to the field for the other papers in this special issue.     

3D Simulations to investigate initial condition effects on the growth of Rayleigh–Taylor mixing

The effect of initial conditions on the growth rate of turbulent Rayleigh–Taylor (RT) mixing has been studied using carefully formulated numerical simulations. An implicit large-eddy simulation (ILES) that uses a finite-volume technique was employed to solve the three-dimensional incompressible Euler equations with numerical dissipation. The initial conditions were chosen to test the dependence of the RT growth parameters (α_b, α_s) on variations in (a) the spectral bandwidth, (b) the spectral shape, and (c) discrete banded spectra. Our findings support the notion that the overall growth of the RT mixing is strongly dependent on initial conditions. Variation in spectral shapes and bandwidths are found to have a complex effect of the late time development of the RT mixing layer, and raise the question of whether we can design RT transition and turbulence based on our choice of initial conditions. In addition, our results provide a useful database for the initialization and development of closures describing RT transition and turbulence.     

Mode Interactions in Four-frequency Degeneracy-lifted Ring Lasers

Abstract

A non-planar ring cavity, with a non-reciprocal bias in the form of the longitudinal Zeeman effect on the gain medium, has been used to demonstrate four-mode bidirectional operation in a He-Ne ring laser at 632·8 nm. It has also been shown that this ring system is sensitive to rotation.     

Sense induction in folksonomies: a review

Folksonomies, often known as tagging systems, such as the ones used on the popular Delicious or Flickr websites, use a very simple Knowledge Organisation System. Users have thus been quick to adopt this system and create extensive annotations on the Web. However, because of the simplicity of the folksonomy model, the semantics of the tags used is not explicit and can only be inferred from their context of use. This is a barrier for the automatic use of such Knowledge Organisation Systems by computers and new techniques have been developed to extract the semantic of the tags. In this article we discuss the drawbacks of some of these approaches and propose a generalization of the different approaches to detect new senses of terms in a folksonomy. Another weak point of the current state of the art in the field is the lack of formal evaluation methodology; we thus propose a novel evaluation framework. We introduce a dataset and evaluation methodology that enable the comparison of results between different approaches to sense induction in folksonomies. Finally we discuss the performances of different approaches to the task of homonymous/polysemous tag detection and synonymous identification.