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排序方式: 共有1113条查询结果,搜索用时 578 毫秒
51.
Tao Li Nianzhi Ning Angelo Iacobino Liangyan Zhang Hui Wang Giovanna Franciosa 《International journal of molecular sciences》2022,23(2)
Previously, a whole-genome comparison of three Clostridium butyricum type E strains from Italy and the United States with different C. botulinum type E strains indicated that the bont/e gene might be transferred between the two clostridia species through transposition. However, transposable elements (TEs) have never been identified close to the bont/e gene. Herein, we report the whole genome sequences for four neurotoxigenic C. butyricum type E strains that originated in China. An analysis of the obtained genome sequences revealed the presence of a novel putative TE upstream of the bont/e gene in the genome of all four strains. Two strains of environmental origin possessed an additional copy of the putative TE in their megaplasmid. Similar putative TEs were found in the megaplasmids and, less frequently, in the chromosomes of several C. butyricum strains, of which two were neurotoxigenic C. butyricum type E strains, and in the chromosome of a single C. botulinum type E strain. We speculate that the putative TE might potentially transpose the bont/e gene at the intracellular and inter-cellular levels. However, the occasional TE occurrence in the clostridia genomes might reflect rare transposition events. 相似文献
52.
Supervised Multivariate Analysis of Hyper-spectral NIR Images to Evaluate the Starch Index of Apples 总被引:4,自引:0,他引:4
Paolo Menesatti Angelo Zanella Stefano D’Andrea Corrado Costa Graziella Paglia Federico Pallottino 《Food and Bioprocess Technology》2009,2(3):308-314
Fruit maturity indexes are crucial in harvest time determination and commercial context. The harvest time of apples, matching
the desired commercial characteristics, is assessed through starch–iodine test in practice. Fruit halves are dipped into iodine
solution and patterns are visually evaluated by experts comparing them to reference charts. Aim of the work was to study the
relationships of near infrared (NIR) spectral images (1,000–1,700 nm), starch/starch-free patterns visually assessed and RGB
color images. Spectral images of 88 Golden Delicious Klon B apples were sampled at seven different maturity stages. Partial
least-squares discriminant analysis (PLSDA) technique was used on hyper-spectral NIR images to classify single pixels using
its NIR reflectance spectrum. The response variable (i.e. the classification for each pixel) was identified through the matching
of single pixel obtained with the color images, segmented in two classes (starch and starch-free), and the NIR hyper-spectral
matrix. Mean hyper-spectral classification obtained through PLSDA modeling on individual apple correctly classified 80.81%
of the total pixels, while the unique model, i.e. a single model including all the fruits, resulted in 66.33%. In the latter
case, the relationship with the RGB classification showed high values (Pearson correlation coefficient r = 0.95). The present work shows the feasibility of NIR imaging spectroscopy as a tool for apple fruit maturity determination,
avoiding expert’s subjective interpretation by traditional starch index assignments. 相似文献
53.
Berardinelli A Cevoli C Silaghi FA Fabbri A Ragni L Giunchi A Bassi D 《Journal of food science》2010,75(7):E462-E468
Abstract: The nondestructive assessment of apricot fruit quality (Bora cultivar) was carried out by means of FT-NIR reflectance spectroscopy in the wavenumber range 12000 to 4000 cm−1. Samples were harvested at four different ripening stages and scanned by a fiber optical probe immediately after harvesting and after a storage of 3 d (2 d at 4 °C and 1 d at 18 °C); the flesh firmness (FF), the soluble solids content (SSC), the acidity (A), and the titratable acidity (malic and citric acids) were then measured by destructive methods. Soft independent modeling of class analogy (SIMCA) analysis was used to classify spectra according to the ripening stage and the storage: partial least squares regression (PLS) models to predict FF, SSC, A, and the titratable acidity were also set-up for both just harvested and stored apricots. Spectral pretreatments and wavenumber selections were conducted on the basis of explorative principal component analysis (PCA). Apricot spectra were correctly classified in the right class with a mean classification rate of 87% (range: 80% to 100%). Test set validations of PLS models showed R2 values up to 0.620, 0.863, 0.842, and 0.369 for FF, SSC, A, and the titratable acidity, respectively. The best models were obtained for the SSC and A and are suitable for rough screening; a lower power prediction emerged for the other maturity indices and the relative predictive models are not recommended. Practical Application : The results of the study could be used as a tool for the assessment of the ripening stage during the harvest and the quality during the postharvest storage of apricot fruits. 相似文献
54.
The multi-scale Gibbs–Helmholtz constrained (GHC) equation is a new predictive cubic equation of state that constrains the energy parameter in the SRK equation to satisfy the Gibbs–Helmholtz equation. It makes use of internal energies of departure calculated from NTP Monte Carlo simulations at the molecular length scale and a novel up-scaling expression to determine the energy parameter at the bulk phase length scale. 相似文献
55.
Nordstrom JL Rangdale R Vickery MC Phillips AM Murray SL Wagley S DePaola A 《Journal of food protection》2006,69(11):2770-2772
Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom. 相似文献
56.
Angelo G. Giumanini Giancarlo Verardo Ennio Zangrando Lucia Lassiani 《Advanced Synthesis \u0026amp; Catalysis》1987,329(6):1087-1103
A product study of the reaction between a number of aromatic amines substituted with widely different groups and paraformaldehyde in inert solvents was performed and found to yield 1,3,5-triaryl-1,3,5-hexahydrotriazines, 1,3,5,7-tetraaryl-1,3,5,7-tetrazocines and formaminals. It was not possible to correlate the product outcomes with the actual structure of the amine substrate. The X-ray diffraction structural determination of 1,3,5-tri-(t-butylphenyl)-( 1b ) and 1,3,5-tri-(m-fluorophenyl)-1,3,5-hexahydrotriazine ( 1c ) showed the diaxial arrangement of the N-substituents. 相似文献
57.
Angelo G. Giumanini Giancarlo Verardo Lucio Randaccio Nevenka Bresciani-Pahor Pietro Traldi 《Advanced Synthesis \u0026amp; Catalysis》1985,327(5):739-748
A rapid and efficient preparation of 1,3,5-triphenylhexahydro-sym-triazine ( 10 ) using paraformaldehyde is reported. The trimeric nature of the compound and its structure in solid state, where a biaxial chair conformation is favoured, are conclusively shown by X-ray diffraction analysis. A recent report on a preparation of 1,3-diphenyl-1,3-diazetidine is invalidated. 相似文献
58.
Lorenza Marinaccio Azzurra Stefanucci Giuseppe Scioli Alice Della Valle Gokhan Zengin Angelo Cichelli Adriano Mollica 《International journal of molecular sciences》2022,23(6)
Elastases are a broad group of enzymes involved in the lysis of elastin, the main component of elastic fibres. They are produced and released in the human body, mainly by neutrophils and the pancreas. The imbalance between elastase activity and its endogenous inhibitors can cause different illnesses due to their excessive activity. The main aim of this review is to provide an overview of the latest advancements on the identification, structures and mechanisms of action of peptide human neutrophil elastase inhibitors isolated from natural sources, such as plants, animals, fungi, bacteria and sponges. The discovery of new elastase inhibitors could have a great impact on the pharmaceutical development of novel drugs through the optimization of the natural lead compounds. Bacteria produce mainly cyclic peptides, while animals provide for long and linear amino acid sequences. Despite their diverse natural sources, these elastase inhibitors show remarkable IC50 values in a range from nM to μM values, thus representing an interesting starting point for the further development of potent bioactive compounds on human elastase enzymes. 相似文献
59.
The literature dedicated to the analysis of the difference in research productivity between the sexes tends to agree in indicating
better performance for men. Through bibliometric examination of the entire population of research personnel working in the
scientific-technological disciplines of Italian university system, this study confirms the presence of significant differences
in productivity between men and women. The differences are, however, smaller than reported in a large part of the literature,
confirming an ongoing tendency towards decline, and are also seen as more noticeable for quantitative performance indicators
than other indicators. The gap between the sexes shows significant sectorial differences. In spite of the generally better
performance of men, there are scientific sectors in which the performance of women does not prove to be inferior. 相似文献
60.