In-House Validation and Comparison of Two Wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis>) Taxon-Specific Real-Time PCR Methods for GMO Quantification Supported by Droplet Digital PCR

Common wheat is one of the most important staple food crops worldwide. However, unlike other important staple crops such as maize or soybean, genetically modified (GM) wheat is not yet present in the global food market. Nonetheless, in the recent past, the adventitious presence of GM glyphosate-tolerant volunteers was reported in open wheat fields in the USA. The European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) was therefore called to develop a strategy to detect such unauthorised GM wheat in wheat samples by using both taxon-specific and screening tests. Two candidate common wheat taxon-specific real-time PCR methods were suggested, one targeting ssII-D gene coding for starch synthase and the other targeting waxy-D1 gene, coding for granule-bound starch synthase. In the present study, the two above-mentioned real-time PCR taxon-specific methods were in-house verified and compared, proposing droplet digital PCR (ddPCR) as a new tool for supporting the application of the European Network of GMO Laboratories (ENGL) established method performance criteria. Preliminary performance data of waxy-D1 and ssII-D methods in ddPCR format are shown too to give a contribution to the bridging process from the consolidated to the emerging quantitative PCR methodology.     

Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells

Some in vitro and in vivo studies have demonstrated protective effects of conjugated linoleic acid (CLA) isomers against oxidative stress and lipid peroxidation. However, only a few and conflicting studies have been conducted showing the antioxidant potential of essential fatty acids. The objectives of the study were to compare the effects of CLA to other essential fatty acids on the thiol redox status of bovine mammary epithelia cells (BME-UV1) and their protective role against oxidative damage on the mammary gland by an in vitro study. The BME-UV1 cells were treated with complete medium containing 50 μM of cis-9,trans-11 CLA, trans-10,cis-12 CLA, α-linolenic acid, γ-linolenic acid, and linoleic acid. To assess the cellular antioxidant response, glutathione, NADPH, and γ-glutamyl-cysteine ligase activity were measured 48 h after addition of fatty acids (FA). Intracellular reactive oxygen species and malondialdehyde production were also assessed in cells supplemented with FA. Reactive oxygen species production after 3 h of H₂O₂ exposure was assessed to evaluate and to compare the potential protection of different FA against H₂O₂-induced oxidative stress. All FA treatments induced an intracellular GSH increase, matched by high concentrations of NADPH and an increase of γ-glutamyl-cysteine ligase activity. Cells supplemented with FA showed a reduction in intracellular malondialdehyde levels. In particular, CLA isomers and linoleic acid supplementation showed a better antioxidant cellular response against oxidative damage induced by H₂O₂ compared with other FA.     

Drying of Inorganic Particulate Compounds

ABSTRACT

Inorganic particulates are usually dried in a fixed bed, fluidized bed, or spray dryers. These compounds are easy to dry, once their physical structure, with high porosity, allows moisture content removal with low resistances. For fluidized bed of alumina particle evaluations, a laboratory-scale drying unit was built. The drying experiments were carried out with alumina particles with different diameters to evaluate temperature and air flow rate effects on drying kinetics and bed height. In another case, the dehydration of a mixture of rare-earth chlorides in a fluidized bed was studied, aiming at the production of anhydrous rare-earth chlorides, used to obtain mischmetal by electrolytic and metallothermic processes. The spray drying experiments were carried out in a pilot plant. Spray drying is a technique largely applied in industrial processes to dry solutions or suspensions, converting their solid parts into a dried powder. A set of rare-earth drying experiments was carried out, aiming at the development of techniques to obtain a powder that could satisfy international morphological requirements. The results allowed evaluating the effects of air flow rate, feed concentration, atomizer model, rotation velocity, and atomization pressure on powder density and particle size distribution.     

Reoptimization of the Shortest Common Superstring Problem

A reoptimization problem describes the following scenario: given an instance of an optimization problem together with an optimal solution for it, we want to find a good solution for a locally modified instance.     

Modelling and performance of a small siphonic hydropower system

A siphon turbine is a low-head, axial-flow hydropower device that can be installed across an impound structure, such as a weir. A laboratory-scale siphon turbine and generator system is built from both off-the-shelf components and bespoke components designed using well-documented guidance. This paper proposes a modelling method for the system, which allows performance to be assessed as a function of operating variables including head, electric load, runner speed and flow rate. An un-modified, commercially available propeller is used for the runner, whose blade geometry is defined within the model as a set of polynomials. This enables flow angles at any radial position on a blade to be determined as a function of the system operating point. Consequently, the impact of design changes on system performance can be quantified, such as part-load efficiency for example. Testing using the laboratory-scale system is conducted up to a maximum gross head of 0.25 m, to validate the component models and then to demonstrate that the final system model provides satisfactory agreement with experiments over the operational speed and head range. The model therefore permits rapid conceptual design to be carried out without the need for computationally intensive co-optimisation of components.     

DOMAIN OF INFLUENCE IN THERMOELASTICITY WITH ONE RELAXATION TIME

A domain-of-influence theorem for an initial-boundary-value problem of thermoelasticity with one relaxation time, proposed by Lord and Schulman, is established. The theorem asserts that for a finite time t > 0 a pair (<φ, ), in which φ and are the thermoelastic potential and temperature, respectively, generates no thermoelastic disturbances outside a bounded domain D_t.     

High performance water-borne paints with high volume solids based on bimodal latexes

The performance of water-borne paints formulated with five different high solids content acrylic (MMA/BA/MAA) latexes was investigated. These latexes had different particle size distributions (unimodal at 61 wt% and bimodal with the large mode below or above 350 nm at 65 wt% solids), different types of surfactant and in one case the carboxylic monomer was omitted.     

Pavement Life-Cycle Cost and Asphalt Binder Quality: Theoretical and Experimental Investigation

Quality control/quality assurance tests can be used to demonstrate that an as-constructed pavement has characteristics different from the contracted specifications of the as-designed pavement because the bitumen employed was of insufficient quality. Many different issues affect asphalt binder quality. Such problems can significantly increase the pavement life-cycle cost, which means that a pay adjustment (PA) is needed. Unfortunately, the quantitative analysis of this issue is very complex. The objectives of this study were to model the dependence of the pavement life-cycle cost on asphalt binder quality and to determine the quantitative relationship between bitumen viscosity and the PA for a given class of boundary conditions. In addition to modeling this problem theoretically, experiments and simulations were carried out through the use of the Mechanistic-Empirical Pavement Design Guide. We then derived the consequences of these effects on life-cycle cost by using a specific life-cycle cost model. Our results demonstrate that asphalt binder viscosity can strongly affect the expected pavement life and the PA, and thus needs to be taken into account in contract and construction management.     

A voltage controlled CMOS current divider with linear characteristic

A compact voltage controlled CMOS current divider is presented. Differently from previous implementations, exploiting the MOSFET translinear characteristics in subthreshold region, the proposed circuit is based on devices operating from moderate to strong inversion. An input current linearity range of nearly a decade is obtained by compensating for the deviations from the ideal drain current square-law approximation through an original empirical approach. The circuit is suitable for use as a building block in analog multipliers and for transconductor continuous tuning. The effectiveness of the proposed configuration is demonstrated by means of electrical simulations, performed on a prototype designed with a commercial process. The robustness of the linearization algorithm with respect to temperature variations is proven.