Quantification of cell adhesion force with AFM: distribution of vitronectin receptors on a living MC3T3-E1 cell

Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy (AFM). First, the distribution of the integrin beta(5) subunit which constitutes a part of the VN receptor on the cell was confirmed by conventional immunohistochemistry after fixing the cell. To visualize the distribution of the receptor on a living cell by an independent and potentially a more quantitative method, the AFM was used with a microbead attached to the cantilever tip to increase the area of contact and VN was immobilized on the microbead. Force measurements were then performed over a large area of a living murine osteoblastic cell using the microbead covered with VN.     

An Automatic Guidance System of a Self-Controlled Vehicle

An automatically guided vehicle, traveling without fixed guide ways, has been developed. In this paper, the construction of the vehicle, the control algorithm, and its general performance are described.     

Daily variation in an intensity of kaolin-induced writhing reaction in mice

The daily variation in an intensity of kaolin-induced writhing reaction was examined in mice kept under conditions of light; 07:00 - 19:00 and dark; 19:00 - 07:00. The number of writhes was counted for 30 minutes after a single intraperitoneal injection of kaolin at 00:00, 02:00, 04:00, 06:00, 08:00, 10:00, 12:00, 14:00, 16:00, 18:00, 20:00 and 22:00. The number of writhes showed a daily variation with a peak at 18:00 and a trough at 06:00. The intensity of writhing reaction was significantly reduced by pretreatment with the bradykinin B1 (Des-Arg9-[Leu8]-BK) and B2 (icatibant) receptor antagonists. Significant daily variation in this parameter was still observed in the group with the B1 antagonist, but disappeared in the B2 antagonist-treated group. These results suggest that the kaolin-induced writhing reaction shows the daily variation with a peak at the end of the resting period and a trough at the end of the active period. The B2 receptor mediated stimuli appears to be involved in this phenomenon.     

A 144-Mb, eight-level NAND flash memory with optimized pulsewidthprogramming

We report a fast-programming, compact sense and latch (SL) circuit to realize an eight-level NAND flash memory. Fast programming is achieved by supplying optimized voltage and pulsewidth to the bit lines, according to the programming data. As a result, all data programming is completed almost simultaneously, and 0.67-MB/s program throughput, which is 1.7 times faster than conventional program throughput, is achieved. The compact layout of the SL circuit is made possible by four 3-bit latches sharing one unit of the read/verify control circuit. Using these techniques, we fabricated a 144-Mb, eight-level NAND flash memory using a 0.35-μm CMOS process, resulting in a 104.2-mm² die size and a 1.05-μm² effective cell size     

Separation of a Nonstationary Component from the EEG by a Nonlinear Digital Filter

A new nonlinear digital filter which separates nonstationary waves such as spikes from stationary background waves of the EEG is proposed. This filter is composed of a prediction filter and a simple nonlinear function. Some examples showing the separation of spikes from EEG data of epileptic patients are given.     

Ethylene carbonate/ether solvents for electrolytes in lithium secondary batteries

An examination has been made of the effectiveness of ethylene carbonate(EC)/2-methyltetrahydrofuran(2-MeTHF) solvents incorporating LiAsF₆ as the solute as electrolytes in secondary lithium batteries. From —10 to 30 °C, the conductivities of EC/2-MeTHF are higher than those of 2-MeTHF and EC/propylene carbonate (PC). For lithium-on-lithium cycling in a half cell, the FOM (figure of merit) of lithium in EC/2-MeTHF has a value 2.2 to 2.7 times higher than that in 2-MeTHF and EC/PC. A coin cell of Li/amorphous V₂O ₅-P₂O₅ with EC/2-MeTHF clearly exhibits higher capacity and longer cycle life than cells with 2-MeTHF or EC/PC. It is concluded that EC/2-MeTHF is a promising electrolyte system for secondary lithium battery applications.     

Power reduction techniques for a 1-Mb ECL-CMOS SRAM with an accesstime of 550 ps and an operating frequency of 900 MHz

This paper describes power reduction circuit techniques in an ultra-high-speed emitter-coupled logic (ECL)-CMOS SRAM. Introduction of a 0.25-μm MOS transistor allows a Y decoder and a bit-line driver to be composed of CMOS circuits, resulting in a power reduction of 34%. Moreover, a variable-impedance load has been proposed to reduce cycle time. A 1-Mb ECL-CMOS SRAM was developed by using these circuit techniques and 0.2-μm BiCMOS technology. The fabricated SRAM has an ultrafast access time of 550 ps and a high operating frequency of 900 MHz with a power dissipation of 43 W     

Low-driving-current temperature-stable 10 Gbit/s operation of p-doped 1.3 /spl mu/m quantum dot lasers between 20 and 90/spl deg/C

Low-driving-current temperature-stable 10 Gbit/s direct modulation was achieved for optimised 200 mum-long short cavity 1.3 mum p-doped quantum dot lasers. Driving conditions were 25.2 mA_p-p for the modulation current and 23.4 mA for the bias current through the whole temperature range from 20 to 90degC     

Novel Conjugated Organic Dyes for Efficient Dye‐Sensitized Solar Cells

Novel conjugated organic dyes that have N,N‐dimethylaniline (DMA) moieties as the electron donor and a cyanoacetic acid (CAA) moiety as the electron acceptor were developed for use in dye‐sensitized nanocrystalline‐TiO₂ solar cells (DSSCs). We attained a maximum solar‐energy‐to‐electricity conversion efficiency (η) of 6.8 % under AM 1.5 irradiation (100 mW cm^–2) with a DSSC based on 2‐cyano‐7,7‐bis(4‐dimethylamino‐phenyl)hepta‐2,4,6‐trienoic acid (NKX‐2569): short‐circuit photocurrent density (J_sc) = 12.9 mA cm^–2, open‐circuit voltage (V_oc) = 0.71 V, and fill factor (ff) = 0.74. The high performance of the solar cells indicated that highly efficient electron injection from the excited dyes to the conduction band of TiO₂ occurred. The experimental and calculated Fourier‐transform infrared (FT‐IR) absorption spectra clearly showed that these dyes were adsorbed on the TiO₂ surface with the carboxylate coordination form. A molecular‐orbital calculation indicated that the electron distribution moved from the DMA moiety to the CAA moiety by photoexcitation of the dye.