Escherichia coli wild type and hydrogenase mutant cells growth and hydrogen production upon xylose and glycerol co-fermentation in media with different buffer capacities

In this study, the new strategy for long term bio-hydrogen (H₂) production using different substrates and waste materials is presented. Growth characteristics and H₂ production were investigated upon consumption of 0.4% xylose and 1% glycerol alone (which were optimal) or their mixture by Escherichia coli BW25113 wild type parental strain (PS) and ΔhyaB, ΔhybC, ΔhycE, ΔhyfG mutants with genes deletions for key subunits of hydrogenase (Hyd)-1 to Hyd-4, respectively, in high and low buffer capacity peptone (HPM, LPM) mediums, pH 5.5 and 7.5. Overall, pH 5.5 negatively affected bacterial growth and H₂ production. At pH 7.5, apart from Hyd-3 and Hyd-4 mutants, upon growth of PS, Hyd-1 and Hyd-2 mutants drop of Pt redox electrode readings from positive (～+150 mV) to negative (of ?400 to ?550 mV) values was detected during log growth phase mentioning H₂ formation. Xylose and glycerol co-utilization did not affect PS and Hyd-1 and Hyd-2 mutant's biomass and H₂ formation during log growth phase in LPM, but ～1.5 fold stimulated these parameters, especially in HPM, pH 7.5, during prolonged 96 h bacterial growth. Roles of Hyd-3 and Hyd-4 in H₂ production; and Hyd-1 and Hyd-2 in H₂ oxidation during bacterial log growth phase were stated under xylose and glycerol co-fermenting conditions. The results obtained might be valuable for industrial long term H₂ production by bacteria using mixture of carbon sources and combining various organic waste materials.     

Instant coffee with high chlorogenic acid levels protects humans against oxidative damage of macromolecules

Scope: Coffee is among the most frequently consumed beverages. Its consumption is inversely associated to the incidence of diseases related to reactive oxygen species; the phenomenon may be due to its antioxidant properties. Our primary objective was to investigate the impact of consumption of a coffee containing high levels of chlorogenic acids on the oxidation of proteins, DNA and membrane lipids; additionally, other redox biomarkers were monitored in an intervention trial. Methods and results: The treatment group (n=36) consumed instant coffee co‐extracted from green and roasted beans, whereas the control consumed water (800 mL/P/day, 5 days). A global statistical analysis of four main biomarkers selected as primary outcomes showed that the overall changes are significant. 8‐Isoprostaglandin F2α in urine declined by 15.3%, 3‐nitrotyrosine was decreased by 16.1%, DNA migration due to oxidized purines and pyrimidines was (not significantly) reduced in lymphocytes by 12.5 and 14.1%. Other markers such as the total antioxidant capacity were moderately increased; e.g. LDL and malondialdehyde were shifted towards a non‐significant reduction. Conclusion: The oxidation of DNA, lipids and proteins associated with the incidence of various diseases and the protection against their oxidative damage may be indicative for beneficial health effects of coffee.     

Artemisia dracunculus L. essential oil phytochemical components trigger the activity of cellular antioxidant enzymes

Investigation of dietary biologically active phytochemicals is of interest due to the availability, low cost, and low rate of side effects of these substances. The main objective of this work was to investigate the influence of the essential oil (EO) extracted from the aerial parts of Artemisia dracunculus on the antioxidant capacity of cells as this plant is one of the most available and widely used as spice and in folk medicine. For this, BV-2 microglial wild type (WT) and acyl-CoA oxidase type 1 (ACOX1) deficient cells (Acox1^−/−) were used. Acox1^−/− cells were applied as the model of cellular oxidative damage. The main component of EO of A. dracunculus was estragole, which was reaching 84.9% in plants cultivated at high altitude Armenian landscape. IC₅₀ value of EO in 1,1-diphenyl-2-picrylhydrazyl assay was 94.2 µg/ml. Sub-cytotoxic concentration in the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test for both BV-2 WT and Acox1^−/− cell lines was 5.10⁻¹ µg/ml. Seventy-two-hours treatment with EO leads to the increased viability (up to 12% in WT and up to 14% -in BV-2 Acox1^−/−cells). The 48-hr treatment increased the ACOX1 activity up to 70% in WT cells. Catalase and superoxide dismutase activities of both cell lines increased following the 24–48-hr treatment. These results indicate that A. dracunculus EO can be considered as a potential protective agent useful in preventive medicine.     

Multi-scale Simulation Methodology for Stress Assessment in 3D IC: Effect of Die Stacking on Device Performance

Potential challenges with managing mechanical stress distributions and the consequent effects on device performance for advanced 3D integrated circuit (IC) technologies are outlined. A set of physics-based compact models for a multi-scale simulation, to assess the mechanical stress across the device layers in silicon chips stacked and packaged with the 3D through-silicon-via (TSV) technology, is proposed. A calibration technique based on fitting to measured stress components and electrical characteristics of the test-chip devices is presented. For model validation, high-resolution strain measurements in Si channels of the test-chip devices are needed. At the nanoscale, the transmission electron microscopy (TEM) is the only technique available for sub-10 nm strain measurements so far.     

Multivariate distribution models with prescribed marginals and covariances

Two multivariate distribution models consistent with prescribed marginal distributions and covariances are presented. The models are applicable to arbitrary number of random variables and are particularly suited for engineering applications. Conditions for validity of each model and applicable ranges of correlation coefficients between the variables are determined. Formulae are developed which facilitate evaluation of the model parameters in terms of the prescribed marginals and covariances. Potential uses of the two models in engineering are discussed.     

Probabilistic Capacity Models and Fragility Estimates for Reinforced Concrete Columns based on Experimental Observations

A methodology to construct probabilistic capacity models of structural components is developed. Bayesian updating is used to assess the unknown model parameters based on observational data. The approach properly accounts for both aleatory and epistemic uncertainties. The methodology is used to construct univariate and bivariate probabilistic models for deformation and shear capacities of circular reinforced concrete columns subjected to cyclic loads based on a large body of existing experimental observations. The probabilistic capacity models are used to estimate the fragility of structural components. Point and interval estimates of the fragility are formulated that implicitly or explicitly reflect the influence of epistemic uncertainties. As an example, the fragilities of a typical bridge column in terms of maximum deformation and shear demands are estimated.     

Monolayer Silane‐Coated,Water‐Soluble Quantum Dots

A one‐step method to produce ≈12 nm hydrodynamic diameter water‐soluble CdSe/ZnS quantum dots (QDs), as well as CdS/ZnS, ZnSe/ZnMnS/ZnS, AgInS₂/ZnS, and CuInS₂/ZnS QDs, by ligand exchange with a near‐monolayer of organosilane caps is reported. The method cross‐links the surface‐bound silane ligands such that the samples are stable on the order of months under ambient conditions. Furthermore, the samples may retain a high quantum yield (60%) over this time. Several methods to functionalize aqueous QD dispersions with proteins and fluorescent dyes have been developed with reaction yields as high as 97%.     

Yeast extract as an effective nitrogen source stimulating cell growth and enhancing hydrogen photoproduction by Rhodobacter sphaeroides strains from mineral springs

The suitability and limitation of yeast extract as nitrogen source to support cell growth and to enhance hydrogen photoproduction by Rhodobacter sphaeroides strains MDC6521 and MDC6522 isolated from mineral springs in Armenia was investigated during the anaerobic growth. Yeast extract (2 g L⁻¹) was indicated to be an effective nitrogen source for bacterial cell growth stimulation and enhanced H₂ production (compared to glutamate). Both strains followed similar growth patterns in medium with yeast extract as nitrogen source and succinate or malate as carbon source. The highest growth rate was obtained for bacterial cells with yeast extract: the latter added gave a stimulated (2–3.5 fold) growth rate than using glutamate. R. sphaeroides suspension oxidation–reduction potential (ORP), which was measured with a platinum electrode, decreased down to low negative values with nitrogen source for both strains. ORP decreased down to more negative values (−610 ± 25 mV) in the presence of yeast extract than when adding glutamate (−405 ± 15 mV) compared to the control (without nitrogen source addition): the significant decrease of ORP indicated enhanced (∼6 fold) H₂ yield. The noticeable ORP decrease measured with the titanium-silicate electrode and simultaneously the increase of extracellular pH ([pH]_out) were observed; ORP was more negative at alkaline [pH]_out. Thus, the optimal culture conditions with nitrogen and carbon sources for bacterial growth stimulation and enhanced H₂ production were established. The ORP decrease together with the increase of [pH]_out point out a significant role of reduction processes in cell growth and ability of bacteria to live.     

Ni (II) and Mg (II) ions as factors enhancing biohydrogen production by Rhodobacter sphaeroides from mineral springs

Various metal ions play a key role in biohydrogen (H₂) production by phototrophic bacteria through incorporation into or stimulating the responsible enzymes and/or related pathways. The Ni (II) and Mg (II) ions effects on growth and H₂ production by Rhodobacter sphaeroides strain MDC6521 isolated from mineral springs in Armenia were established. The highest growth specific rate was obtained with 4–6 μM Ni²⁺ and 5 mM Mg²⁺. pH of the growth medium changed from 7.0 to 9.2–9.4 during the bacterial growth up to 72 h in spite of Ni²⁺ added but pH increased in different manner with Mg²⁺. In the presence of 2–4 μM Ni²⁺ external oxidation-reduction potential (ORP) decreased to more negative values (−800 ± 15 mV). This decrease of ORP indicated ∼2.7-fold enhanced H₂ yield (9.80 mmol L⁻¹) with Ni²⁺ compared with the control (without Ni²⁺). The H₂ yield determined in the medium with Mg²⁺ was ∼2.2 fold higher than that with 1 mM Mg²⁺. These results reveal new regulatory ways to improve H₂ production by R. sphaeroides those were depending on Ni²⁺ and Mg²⁺ of different concentrations.