Quantitative analysis of sperm rheotaxis using a microfluidic device

Quite puzzling issue in biology is how sperm cells are selected naturally where human sperm has to maintain a correct swimming behavior during the various stages of reproduction process. In nature, sperm has to compete a long journey from cervix to oocyte to stand a chance for fertilization. Although various guidance mechanisms such as chemical and thermal gradients are proposed previously, these mechanisms may only be relevant as sperm reaches very close to the oocyte. Rheotaxis, a phenomenon where sperm cells swim against the flow direction, is possibly the long-range sperm guidance mechanism for successful fertilization. A little is known quantitatively about how flow shear effects may help guide human sperm cells over long distances. Here, we have developed microfluidic devices to quantitatively investigate sperm rheotaxis at various physiological flow conditions. We observed that at certain flow rates sperm actively orient and swim against the flow. Sperm that exhibit positive rheotaxis show better motility and velocity than the control (no-flow condition), however, rheotaxis does not select sperm based on hyaluronic acid (HA) binding potential and morphology. Morphology and HA binding potential may not be a significant factor in sperm transport in natural sperm selection.     

A hybrid lithium‐ion battery model for system‐level analyses

We describe an advanced lithium‐ion battery model for system‐level analyses such as electric vehicle fleet simulation or distributed energy storage applications. The model combines an empirical multi‐parameter model and an artificial neural network with particular emphasis on thermal effects such as battery internal heating. The model is fast and can accurately describe constant current charging and discharging of a battery cell at a variety of ambient temperatures. Comparison to a commonly used linear kilowatt‐hour counter battery model indicates that a linear model overestimates the usable capacity of a battery at low temperatures. We highlight the importance of including internal heating in a battery model at low temperatures, as more capacity is available when internal heating is taken into account. Copyright © 2016 John Wiley & Sons, Ltd.     

Analytic investigation of effect of electric field on elasto-plastic response of a functionally graded piezoelectric hollow sphere

In this work, the effect of electric potential on the mechanical (Stresses, strains, displacement) and electrical (electrical displacement and intensity) response of a Functionally graded piezoelectric (FGP) hollow sphere is analytically investigated. The sphere is under the action of internal/external pressure and temperature gradient as well. The inhomogeneity is based on power law in radial direction. The analysis is done in two parts: elastic response and plastic response, using Tresca yield criterion. It is shown by illustrative example that under internal pressure and assumed model parameters, the commencement of plastic region is from outside surface towards inside in the plastic zone is extended with the increase of electric potential. Interestingly, radial stress and displacement have an extreme not on the boundaries, but on the inside.     

A mixed analytical-numerical investigation of snap-through of low arches with a power-law variable thickness

Snap-through is an instability phenomenon that occurs in arch and dome-shaped structures, wherein the structure has to move from a stable equilibrium state through an unstable path into another stable equilibrated configuration in a jumping action. In this study, a linear elastic isotropic low arch is considered as a structure with power-law variable thickness. The phenomenon is investigated by considering the equation of the deflection for the variable thickness arch, solving it with an elegant analytical technique, and finding the snap-through critical load from an extreme condition. The effect of power-law exponent and geometry of the arch centerline on critical load is investigated and the constant thickness case and a very rare case of power-law thickness variation found in literature are considered for verification.     

CSH RNA Interference Reduces Global Nutrient Uptake and Umbilical Blood Flow Resulting in Intrauterine Growth Restriction

Deficiency of the placental hormone chorionic somatomammotropin (CSH) can lead to the development of intrauterine growth restriction (IUGR). To gain insight into the physiological consequences of CSH RNA interference (RNAi), the trophectoderm of hatched blastocysts (nine days of gestational age; dGA) was infected with a lentivirus expressing either a scrambled control or CSH-specific shRNA, prior to transfer into synchronized recipient sheep. At 90 dGA, umbilical hemodynamics and fetal measurements were assessed by Doppler ultrasonography. At 120 dGA, pregnancies were fitted with vascular catheters to undergo steady-state metabolic studies with the ³H₂O transplacental diffusion technique at 130 dGA. Nutrient uptake rates were determined and tissues were subsequently harvested at necropsy. CSH RNAi reduced (p ≤ 0.05) both fetal and uterine weights as well as umbilical blood flow (mL/min). This ultimately resulted in reduced (p ≤ 0.01) umbilical IGF1 concentrations, as well as reduced umbilical nutrient uptakes (p ≤ 0.05) in CSH RNAi pregnancies. CSH RNAi also reduced (p ≤ 0.05) uterine nutrient uptakes as well as uteroplacental glucose utilization. These data suggest that CSH is necessary to facilitate adequate blood flow for the uptake of oxygen, oxidative substrates, and hormones essential to support fetal and uterine growth.     

Influence of compliant walls on peristaltic motion with heat/mass transfer and chemical reaction

In this work the fundamental problem of peristalsis with heat and mass transfer is investigated in the presence of a chemical reaction. An incompressible viscous fluid is considered in a channel with compliant walls. Mathematical modeling is based upon the laws of mass, linear momentum, energy and concentration. Analysis is presented not only for long wavelength and low Reynolds number but also for small Grashof number. The solutions are carried out for the stream function, temperature, concentration field and heat transfer coefficient. The results of various interesting parameters are plotted and discussed.     

Experimental Measurement of Asymmetric Fluctuations of Poloidal Magnetic Field in Damavand Tokomak at Different Plasma Currents

Toroidal and Poloidal magnetic fields have an important effect on the tokomak topology. Damavand Tokomak is a small size tokomak characterized with k?=?1.2, B _t?=?1T, R ₀?=?36?cm, maximum plasma current is about 35?KA with a discharge time of 21?ms. In this experimental work, the variation of poloidal magnetic field on the torodial cross section is measured and analyzed. In order to measure the polodial magnetic field, 18 probes were installed on the edge of tokomak plasma with ?θ?=?18°, while a limiter was installed inside the torus. Plasma current, I _p, induces a polodial magnetic field, B _p, smaller than the torodial magnetic field B _t. Magnetic lines B produced as a combination of B _t and B _p, are localized on the nested toroidal magnetic surfaces. The presence of polodial magnetic field is necessary for particles confinement. Mirnov oscillations are the fluctuations of polodial magnetic field, detected by magnetic probes. Disrupted instability in Tokomak typically starts with mirnov oscillations which appear as fluctuations of polodial magnetic field and is detected by magnetic probes. Minor disruptions inside the plasma can contain principal magnetic islands and their satellites can cause the annihilation of plasma confinement. Production of thin layer of turbulent magnetic field lines cause minor disruption. Magnetic limiter may cause the deformation of symmetric equilibrium configuration and chaotic magnetic islands reveal in plasma occurring in thin region of chaotic field lines close to their separatrix. The width of this chaotic layer in the right side of poloidal profile of Damavand Tokomak is smaller than the width in the left side profile because of Shafranov displacement. Ergodic region in the left side of profile develops a perturbation on the magnetic polodial field lines, B _p, that are greater in magnitude than that in the right side, although the values of B _p on the left side are smaller than that on the right side of the profile. The Left side of profile is close to the principal magnetic axis and the right side is away from Z axis of Tokamak.