Conformation in solution of side-chain liquid crystal polymers as a function of the mesogen-graft amount

The conformation of side-chain liquid crystal polymers with different mesogen-graft amounts has been studied by small-angle neutron scattering in dilute solutions of toluene-d8 and THF-d8. It is shown that the radius of gyration increases by about twofold when the mesogen-graft amount increases from 0 to 100%, which suggests that the persistence length of the backbone increases by about 4-fold. Comparison with the results reported in the literature on the melt state suggests that the persistence length is not an intrinsic property of side chain liquid crystal polymers, but depends on inter-chain interactions.     

Resistive Switching of Individual,Chemically Synthesized TiO2 Nanoparticles

Resistively switching devices are considered promising for next‐generation nonvolatile random‐access memories. Today, such memories are fabricated by means of “top–down approaches” applying thin films sandwiched between nanoscaled electrodes. In contrast, this work presents a “bottom–up approach” disclosing for the first time the resistive switching (RS) of individual TiO₂ nanoparticles (NPs). The NPs, which have sizes of 80 and 350 nm, respectively, are obtained by wet chemical synthesis and thermally treated under oxidizing or vacuum conditions for crystallization, respectively. These NPs are deposited on a Pt/Ir bottom electrode and individual NPs are electrically characterized by means of a nanomanipulator system in situ, in a scanning electron microscope. While amorphous NPs and calcined NPs reveal no switching hysteresis, a very interesting behavior is found for the vacuum‐annealed, crystalline TiO_2–x NPs. These NPs reveal forming‐free RS behavior, dominantly complementary switching (CS) and, to a small degree, bipolar switching (BS) characteristics. In contrast, similarly vacuum‐annealed TiO₂ thin films grown by atomic layer deposition show standard BS behavior under the same conditions. The interesting CS behavior of the TiO_2–x NPs is attributed to the formation of a core–shell‐like structure by re‐oxidation of the reduced NPs as a unique feature.     

Evaluation of DNA extraction methods for Bacillus anthracis spores spiked to food and feed matrices at biosafety level 3 conditions

The DNA extraction efficiency from milk, whey, soy, corn gluten meal, wheat powders and heat-treated corn grain that were spiked with Bacillus anthracis and Bacillus thuringiensis spores was determined. Two steps were critical: lysis of the spores and binding of the free DNA to the DNA binding magnetic beads in the presence of the interfering powders. For the guanidine-thiocyanate based Nuclisens lysis buffer from Biomerieux we found that between 15 and 30% of the spores survived the lysis step. As most lysis buffers in DNA/RNA extraction kits are guanidine based it is likely that other lysis buffers will show a similar partial lysis of the Bacillus spores. Our results show that soybean flour and wheat flour inhibited the DNA extraction process strongest, leading to unreliable DNA extractions when using too much of the matrix. For corn gluten meal, heat-treated corn grain and milk powders, DNA extraction efficiencies in the presence of 100mg and 10mg of powder resulted in 70%-95% reduced DNA recoveries. The inhibition was, however, reliable and intermediate compared to the inhibition by soy and wheat. Whey powder had the lowest inhibitory effect on DNA-extraction efficiency and recoveries of 70-100% could be reached when using 10mg of powder. The results show that reducing the amount of matrix leads to better DNA-extraction efficiencies, particularly for strongly inhibiting powders such as soy and wheat. Based on these results, a standard protocol to directly isolate DNA from micro-organisms present in complex matrixes such as food and feed powders was designed.     

Large‐scale Synthesis of Urchin‐like Mesoporous TiO2 Hollow Spheres by Targeted Etching and Their Photoelectrochemical Properties

A versatile targeted etching strategy is developed for the large‐scale synthesis of urchin‐like mesoporous TiO₂ hollow spheres (UMTHS) with tunable particle size. Its key feature is the use of a low‐temperature hydrothermal reaction of surface‐fluorinated, amorphous, hydrous TiO₂ solid spheres (AHTSS) under the protection of a polyvinylpyrrolidone (PVP) coating. With the confinement of PVP and water penetration, the highly porous AHTSS are selectively etched and hollowed by fluoride without destroying their spherical morphology. Meanwhile TiO₂ hydrates are gradually crystallized and their growth is preferentially along anatase (101) planes, reconstructing an urchin‐like shell consisting of numerous radially arranged single‐crystal anatase nanothorns. Complex hollow structures, such as core–shell and yolk–shell structures, can also be easily synthesized via additional protection of the interior by pre‐filling AHTSS with polyethylene glycol (PEG). The hollowing transformation is elucidated by the synergetic effect of etching, PVP coating, low hydrothermal reaction temperature, and the unique microstructure of AHTSS. The synthesized UMTHS with a large surface area of up to 128.6 m² g^‐1 show excellent light‐harvesting properties and present superior performances in photocatalytic removal of gaseous nitric oxide (NO) and photoelectrochemical solar energy conversion as photoanodes for dye‐sensitized mesoscopic solar cells.     

The intelligent help system COMFOHELP

The paper is concerned with the question of whether and under what conditions active help systems with plan recognition components that have been developed in the environment of artificial intelligence research are able to prove their value in the real context of commercial application programs. The question is investigated using the development of the COMFOHELP intelligent help system as an example. COMFOHELP supports the COMFOTEX graphical text processing program and has been developed by the Linguistic Information Science Group at the University of Regensburg since 1988. The system recognizes erroneous and suboptimal plans pursued by the user by analyzing the dialog history and comparing them with the correct plan for achieving the user's goal.Section 2 discusses the research situation and elaborates on those problems which up to now prevented research concepts for plan recognition and intelligent help systems from being practically applied. Testing error situations empirically is a first prerequisite since potential erroneous plans can only be established in real-world tests. The second prerequisite is a special system architecture which counteracts the problem of ambiguities in plan recognition. Section 3 introduces a first still restricted prototype version of COMFOHELP whose efficiency was verified in a statistical hypothesis test. The users performing their text processing tasks with the support of COMFOHELP came off significantly better than members of a reference group working without the intelligent help. Section 4 shows that the proposed COMFOHELP system architecture is reconfirmed by the results of extensive empirical investigations (with more than 100 users) of erroneous plans when using a more complex version of COMFOTEX. The architecture still proves to be worthwhile even when functionality is increased by a factor of three to four.     

Effects of practice on speed of information processing in children and adults: Age sensitivity and age invariance.

In 2 experiments, children and adults were exposed to 4 different information-processing tasks. Consistent with the global trend hypothesis, age-sensitive linear relations were observed between child and adult latencies, and 10- and 11-yr-olds were approximately 1.7 and 1.6 times slower than 19-yr-olds as predicted by R. Kail's (see record 1991-20909-001) growth function. In Exp 1, the relation between child and adult latencies did not change over 4 sessions of practice, implying that practice has equivalent effects on corresponding processing steps in children and adults. In both experiments, an age-invariant linear relation between dispersion and central tendency was observed, indicating that children's greater within-S variability is entirely due to their slower speed of processing. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Feasibility of the use of chilean nitrate as a nitrogen source for the growth of Chlorella pyrenoidosa

Potassic Chilean nitrate, a cheap natural product of northern Chile, was found to support the growth of the unicellular green alga Chlorella pyrenoidosa, a possible sheep fodder supplement in this region. Comparisons with conventional media containing urea or KNO₃ were favourable.     

Retrospective patient dose analysis of a digital radiography system in routine clinical use

The log files generated in the flat-panel detector based digital radiography systems (Philips Digital Diagnost) at 11 different hospitals were used to acquire data regarding the dose-area product (DAP) and the entrance air kerma (EAK) for all X-ray examinations performed in routine clinical use. A retrospective statistical analysis of the data with respect to the dose levels was performed for several examination types. The resulting average dose levels were compared with the diagnostic reference levels (DRLs). While for the same type of examination the median dose level at different hospitals could differ by up to a factor of 5, almost all the dose levels were found to be much below the published DRL. Only in one hospital for one type of examination (Thorax PA) the DRL for the DAP was slightly exceeded, while for the same site and examination the EAK was still below the DRL. In conclusion, examination log files can be used for retrospective dose analysis and correlation with DRLs.     

Urine testing for designer steroids by liquid chromatography with androgen bioassay detection and electrospray quadrupole time-of-flight mass spectrometry identification

New anabolic steroids show up occasionally in sports doping and in veterinary control. The discovery of these designer steroids is facilitated by findings of illicit preparations, thus allowing bioactivity testing, structure elucidation using NMR and mass spectrometry, and final incorporation in urine testing. However, as long as these preparations remain undiscovered, new designer steroids are not screened for in routine sports doping or veterinary control urine tests since the established GC/MS and LC/MS/MS methods are set up for the monitoring of a few selected ions or MS/MS transitions of known substances only. In this study, the feasibility of androgen bioactivity testing and mass spectrometric identification is being investigated for trace analysis of designer steroids in urine. Following enzymatic deconjugation and a generic solid-phase extraction, the samples are analyzed by gradient LC with effluent splitting toward two identical 96-well fraction collectors. One well plate is used for androgen bioactivity detection using a novel robust yeast reporter gene bioassay yielding a biogram featuring a 20-s time resolution. The bioactive wells direct the identification efforts to the corresponding well numbers in the duplicate plate. These are subjected to high-resolution LC using a short column packed with 1.7-microm C18 material and coupled with electrospray quadrupole time-of-flight mass spectrometry (LC/QTOFMS) with accurate mass measurement. Element compositions are calculated and used to interrogate electronic substance databases. The feasibility of this approach for doping control is demonstrated via the screening of human urine samples spiked with the designer anabolic steroid tetrahydrogestrinone. Application of the proposed methodology, complementary to the established targeted urine screening for known anabolics, will increase the chance of finding unknown emerging designer steroids, rather then being solely dependent on findings of the illicit preparations themselves.