Non-linear analysis for anisotropic materials

An elasto-plastic analysis of anisotropic work-hardening materials, using the finite element method is presented. The analysis is based on the generalized Huber-Mises yield criterion extended by Hill for anisotropic materials. General expressions for the anisotropic parameters in the yield criterion have been derived both for initial yielding as well as subsequent yielding in the case of work-hardening materials. The isoparametric ‘quadratic’ quadrilateral elements have been used for the analysis and the ‘initial stress technique’ has been adopted for the iterative solution of the non-linear problems. The results of the various numerical examples have been compared with the available solutions.     

Optical and magneto-optical properties of copper-nickel compound metal island films

The optical properties of copper Cu-Ni compound metal island (CMI) films composed of nanoclusters of an alloy of Cu and Ni have been experimentally investigated. The spectral characteristics of the Cu-Ni CMI films are intermediate between those of conventional Cu and Ni island films in visible and near-IR regions. In addition, the magneto-optic effect has been observed in the Cu-Ni CMI films. It is also shown that the Faraday rotation in the Cu-Ni CMI films can easily be controlled by selection of the mixture ratio of Cu and Ni.     

Numerical approach to hopping conduction

A numerical method for calculation of the polarization conductivity has been developed. This approach has been applied to the correlated barrier hopping (CBH) and quantum mechanical tunnelling (QMT) models in the case of monoelectronic hops. These calculations have been performed on well-known solids such as carbon (HTT 600° C) and badly organized molybdenum and tungsten sulphides. The agreement between theory and experimental results is more satisfying than in a previous approach. The parametersW _M andN ₀ are determined for the CBH model, andN(E _F) for the QMT model.     

Design of fractal patch antenna for size and radar cross-section reduction

This research study presents a novel design of star-shaped fractal patch antenna for miniaturisation and backscattering radar cross-section (RCS) reduction. The proposed fractal antenna gives 50% size reduction compared with a conventional circular microstrip patch (CCMP) antenna. The antenna is studied experimentally for return loss behaviour using vector network analyser R&S ZVA40. It can be useful for wireless application in 0.85-4 GHz frequency band. Further, the study focuses on backscattering RCS (both monostatic and bistatic) reduction by the proposed antenna compared with the CCMP antenna. It is found that increase in number of fractal iterations included in the conventional patch to design fractal antenna geometry reduces backscattering RCS at multiband compared to the conventional patch antenna. This reduction in backscattering RCS by the antenna is observed at multiband. The antenna can be tuned for low backscattering by variation in the substrate dielectric constant and thickness and the superstrate dielectric constant and thickness. For maximum RCS reduction by the antenna, optimisation of substrate thickness becomes necessary. The study also deals with effect of frequency and aspect angle variation on backscattering RCS reduction.     

MicroRNA Expression in Clear Cell Renal Cell Carcinoma Cell Lines and Tumor Biopsies: Potential Therapeutic Targets

This study was carried out to quantitate the expression levels of microRNA-17, -19a, -34a, -155, and -210 (miRs) expressed in nine clear cell renal cell carcinoma (ccRCC) and one chromophobe renal cell carcinoma cell line with and without sarcomatoid differentiation, and in six primary kidney tumors with matching normal kidney tissues. The data in the five non-sarcomatoid ccRCC cell lines—RC2, CAKI-1, 786-0, RCC4, and RCC4/VHL—and in the four ccRCC with sarcomatoid differentiation—RCJ41T1, RCJ41T2, RCJ41M, and UOK-127—indicated that miR-17 and -19a were expressed at lower levels relative to miR-34a, -155, and -210. Compared with RPTEC normal epithelial cells, miR-34a, miR-155, and miR-210 were expressed at higher levels, independent of the sarcomatoid differentiation status and hypoxia-inducible factors 1α and 2α (HIFs) isoform expression. In the one chromophobe renal cell carcinoma cell line, namely, UOK-276 with sarcomatoid differentiation, and expressing tumor suppressor gene TP53, miR-34a, which is a tumor suppressor gene, was expressed at higher levels than miR-210, -155, -17, and -19a. The pilot results generated in six tumor biopsies with matching normal kidney tissues indicated that while the expression of miR-17 and -19a were similar to the normal tissue expression profile, miR-210, -155, -and 34a were expressed at a higher level. To confirm that differences in the expression levels of the five miRs in the six tumor biopsies were statistically significant, the acquisition of a larger sample size is required. Data previously generated in ccRCC cell lines demonstrating that miR-210, miR-155, and HIFs are druggable targets using a defined dose and schedule of selenium-containing molecules support the concept that simultaneous and concurrent downregulation of miR-210, miR-155, and HIFs, which regulate target genes associated with increased tumor angiogenesis and drug resistance, may offer the potential for the development of a novel mechanism-based strategy for the treatment of patients with advanced ccRCC.     

CtpB Facilitates Mycobacterium tuberculosis Growth in Copper-Limited Niches

Copper is required for aerobic respiration by Mycobacterium tuberculosis and its human host, but this essential element is toxic in abundance. Copper nutritional immunity refers to host processes that modulate levels of free copper to alternately starve and intoxicate invading microbes. Bacteria engulfed by macrophages are initially contained within copper-limited phagosomes, which fuse with ATP7A vesicles that pump in toxic levels of copper. In this report, we examine how CtpB, a P-type ATPase in M. tuberculosis, aids in response to nutritional immunity. In vitro, the induced expression of ctpB in copper-replete medium inhibited mycobacterial growth, while deletion of the gene impaired growth only in copper-starved medium and within copper-limited host cells, suggesting a role for CtpB in copper acquisition or export to the copper-dependent respiration supercomplex. Unexpectedly, the absence of ctpB resulted in hypervirulence in the DBA/2 mouse infection model. As ctpB null strains exhibit diminished growth only in copper-starved conditions, reduced copper transport may have enabled the mutant to acquire a “Goldilocks” amount of the metal during transit through copper-intoxicating environments within this model system. This work reveals CtpB as a component of the M. tuberculosis toolkit to counter host nutritional immunity and underscores the importance of elucidating copper-uptake mechanisms in pathogenic mycobacteria.     

Microstructure characterization of CVI-densified carbon/carbon composites with various fiber distributions

Mechanical behavior of multi-phase composites is crucially influenced by volume fractions, orientation distributions and geometries of microconstituents. In the case of carbon–carbon composites manufactured by chemical vapor infiltration, the microconstituents are carbon fibers, pyrolytic carbon matrix, and pores. The local variable thickness of the pyrolytic carbon coating, distribution of the fibers and porosity are the main factors influencing the properties of these materials. Two types of fiber arrangements are considered in this paper: 2D laminated preform and random felt. The materials are characterized by determining their densities and their fiber distribution functions, by establishing types of pyrolytic carbon matrix present in the composites, and by studying the porosity. A technique utilizing X-ray computed tomography for estimation of the orientation distribution of the fibers and pores with arbitrary shapes is developed. A methodology based on the processing of microstructure images with subsequent numerical simulation of the coating growth around the fibers is proposed for estimation of the local thickness of the coating. The obtained information is appropriate for micromechanical modeling and prediction of the overall thermo-mechanical properties of the studied composites.