Mechanisms responsible for resistance of sublines derived from leukemia cell lines to an antitumor agent 9-beta-D-arabinofuranosyl-2-fluoroadenine

An agent 9-beta-D-arabinofuranosyl-2-fluoroadenine (2-F-Ara-A) is a main metabolite of fludarabine, a fluorinated purine analogue with antitumor activity in lymphoproliferative malignancies. In this study, the mechanism responsible for the resistance of cancer cells to fludarabine was examined using the 2-F-Ara-A-resistant sublines JOK-1/F-Ara-A and L1210/F-Ara-A from a human hairy leukemic cell line (JOK-1) and a mouse leukemic cell line (L1210) respectively, which were established by continuous treatment of the parental cell lines with 2-F-AraA. JOK-1/F-Ara-A and L1210/F-Ara-A cells were more than 55 and 29 times more resistant to 2-F-Ara-A than were their parent cell lines, and showed a high cross-resistance to 1-beta-D-arabinofuranosylcytosine but not to doxorubicin or vincristine. These resistant sublines intracellularly accumulated almost the same amount of 2-F-Ara-A as did their parent cell lines. However, the amount of 2-F-Ara-ATP, a cytotoxic metabolite of 2-F-Ara-A, decreased by 2.6% (JOK-1/F-Ara-A C3), 6% (L1210/F-Ara-A C1) and 3.7% (L1210/F-Ara-A C7) relative to the levels in the parent cell lines. Enzymatically, these resistant cells hardly activated deoxycytidine (dCyd) and 2-F-Ara-A. In addition, the abilities to phosphorylate deoxyadenosine and deoxyguanosine were also decreased in the resistant cells in comparison with the parent cells. These findings suggest that the deficiency in activity of dCyd kinase may contribute to the resistance of 2-F-Ara-A.     

基于MVC架构文档报表管理系统的设计与实现

根据公司对文档报表管理的实际需求,通过对某房地产评估公司的日常报告文档管理的各方面的需求分析,将系统的需求抽象化、模块化、系统化,利用.NET开发平台MVC架构实现了报告信息管理、数据维护管理、公司收据和报告文档管理等功能,实现了管理文档的规范化和信息化,提高公司管理文档信息的工作质量、管理水平,为用户提供更为方便快捷的服务。     

复杂场景实时目标检测方法

针对含有非平稳背景的复杂场景,提出一种基于空间混合高斯模型的实时目标检测方法.该方法以混合高斯模型作为颜色分布的统计框架,并在空间邻域中利用背景与前景隶属度之间的竞争确定像素的归属,提高了检测准确率;同时通过基于偏差均值的匹配判断和低权重模型的移除提升了混合高斯统计框架的性能,以实时地对运动目标进行准确的检测.实验结果表明,文中方法对非平稳背景有很好的适应能力,在检测准确率和运行效率上均优于其他检测方法.     

基于仿人足球机器人协作定位技术研究

本文综述多机器人协作定位的研究现状,分析比较当前该领域的主要研究方向,介绍实现机器人协作定位的主要研究方法和关键技术,并总结多机器人协作定位面临的挑战和发展趋势.     

Entity-level sentiment prediction in Danmaku video interaction

The Journal of Supercomputing - Sentiment analysis in Danmaku video interaction aims at measuring public mood in respect of the video, which is helpful for the potential applications in behavioral...     

基于ZigBee和GPRS网络的温室大棚无线监测系统设计

为了监测温室大棚的温湿度、CO2浓度、土壤PH值及光照强度等参数,设计了一套基于ZigBee和GPRS网络的温室大棚无线监控系统;多个传感器网络节点采集温室大棚的环境参数,并通过ZigBee网络传给协调器节点,最后利用GPRS网络将数据发送到监控终端,以便农业人员调节温室大棚的环境;介绍了系统的总体设计方案,详细讲述了系统的硬件设计、网络协议的建立及软件的实现,经过现场实际运行测试,该系统长时问工作稳定可靠,具有一定的市场价值和很好的发展前景.     

基于PLC的火电厂电除尘器振打控制的研究

为了提高火电厂中静电除尘器的稳定性、可靠性、自动化水平,采用了高性能施耐德Twido可编程控制器(PLC)以及Twidosoft编程软件,设计了电除尘器电机振打控制方案,编制了振打控制梯形图程序;当沉降室侧壁振打3分钟,停7分钟;第一电场振打2分钟,停8分钟;第二电场振打2分钟,停18分钟;第三电场振打2分钟,停28分钟时,振打效果较好;运行结果表明:采用Twido可编程控制器之后,实现了电机振打的自动运行,与传统继电器振打控制系统相比,可靠性高,稳定性好,除尘效率高,粉尘排放浓度低于50mg/m3,为火电厂静电除尘器电机振打系统的自动控制提供了新的方法。     

空管系统中键盘快捷功能的研究与实现

目前国内空中交通管制系统人机界面的操作越来越趋于人性化,从可用、能用到好用的人机界面发展过程中,软件提供快捷的功能和方便的操作流程起到了很重要的作用,因此研究软件开发对键盘快捷键的支持就显得尤其重要。本文对可以实现的方法进行比较,分析实现方法的优劣,提出了一种实现键盘快捷功能的方法。     

Infants' sensitivity to optical flow for controlling posture.

Infants' sensitivity to optical flow for controlling sitting and standing was tested using a "moving room" in which all of the walls moved together, or only the side walls or front wall moved. Two questions motivated this research. (a) is the optical flow necessary for inducing postural compensations spatially distributed in the optic array? (b) Do visually induced compensations follow a developmental progression, or alternatively, emerge all at once? Experiment 1 was designed to test postural compensations by 14-month-old infants capable of standing in the moving room. Experiment 2 was designed to test postural compensations by 5- to 9-month-old infants who were passively supported while sitting in the moving room. The results revealed that partial flow is generally sufficient for inducing postural compensations, but that the amplitude and consistency of the response depend on the location of the flow in the optic array. In addition, there was evidence suggesting that compensatory responses become increasingly systematic during the second half of the first year. (PsycINFO Database Record (c) 2010 APA, all rights reserved)