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11.
Malatesta M Frigato E Baldelli B Battistelli S Foà A Bertolucci C 《Microscopy research and technique》2007,70(7):578-584
Reptiles represent an interesting animal model to investigate the influence of temperature on molecular circadian clocks. The ruin lizard Podarcis sicula lives in a continental climate and it is subjected to wide range of environmental temperatures during the course of the year. As consequence, ruin lizard daily activity pattern includes either the hibernation or periods of inactivity determined by hypothermia. Here we showed the rhythmic expression of two clock genes, lPer2 and lClock, in the liver of active lizards exposed to summer photo-thermoperiodic conditions. Interestingly, the exposition of lizards to hypothermic conditions, typical of winter season, induced a strong dampening of clock genes mRNA rhythmicity with a coincident decrease of levels. We also examined the qualitative and quantitative distribution of lPER2 and lCLOCK protein in different cellular compartments during the 24-h cycle. In the liver of active lizards both proteins showed a rhythmic expression profile in all cellular compartments. After 3 days at 6 degrees C, some temporal fluctuations of the lCLOCK and lPER2 are still detectable, although, with some marked modifications in respect to the values detected in the liver of active lizards. Besides demonstrating the influence of low temperature on the lizard liver circadian oscillators, present results could provide new essential information for comparative studies on the influence of temperature on the circadian system across vertebrate classes. 相似文献
12.
Changgeng Liu Beatrice Pazzucconi Juan Liu Xincheng Yao 《Journal of Modern Optics》2019,66(12):1311-1317
We demonstrated the feasibility of using a holographic waveguide imager for eye tracking. A holographic waveguide placed in front of the eye was used to capture images of the anterior segment of the eye and to guide the images to a camera distant from the eye. The pupil centre (PC) and corneal reflection (CR) of the eye was used to compute eye position. A custom-built model eye was used to validate the prototype eye tracker. A linear relationship between the angular eye position and the PC/CR vector was found over 60 horizontal degrees and 40 vertical degrees. The tracking accuracy and precision were 0.72 degree and 0.50 degree over these tracking ranges. These results confirmed that holographic waveguide could be a viable platform for developing compact, wearable, see-through eye trackers that can continuously monitor eye movements during real life tasks and thus can facilitate diagnosis of oculomotor disorders. 相似文献
13.
Romain Cauchois Mohamed Saadaoui Abdelwahhab Yakoub Karim Inal Beatrice Dubois-Bonvalot Jean-Christophe Fidalgo 《Journal of Materials Science》2012,47(20):7110-7116
The effect of thermal profile on microstructure is studied in the frame of thin films deposited by inkjet-printing technology. The role of sintering temperature and thermal ramp is particularly investigated. Fast heating ramps exhibit coarse grains and pores, especially when a hybrid microwave curing is performed. This enhanced growth is attributed to the quick activation of densifying sintering regimes without undergoing thermal energy loss at low temperature. Microstructural evolution of various sintered inkjet-printed films is correlated with electrical resistivity and with the Young’s modulus determined by nanoindentation. A strong link between those three parameters is highlighted during experiments giving credit to either a surface or a fully volumetric sintering, according to the process. Sintering is then mainly triggered by surface mass transfer or by grain boundary diffusion. Silver thin films with an electrical resistivity 4–5 times higher than the bulk, has been reached in a few minutes with a Young’s modulus of 38?GPa. 相似文献
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We consider the problem of solving a rational matrix equation arising in the solution of G-networks. We propose and analyze two numerical methods: a fixed point iteration and the Newton–Raphson method. The fixed point iteration is shown to be globally convergent with linear convergence rate, while the Newton method is shown to have a local convergence, with quadratic convergence rate. Numerical experiments show the effectiveness of the proposed methods. 相似文献
17.
Expanding the application field of post‐consumer poly(ethylene terephthalate) through structural modification by reactive blending
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With the aim of up‐grading the material properties of post‐consumer PET, making them suitable for extrusion of thermoformable thick sheets, a series of polyepoxy chain extenders have been comparatively evaluated as melt viscosity modifiers for a toughened compatibilized blend containing up to 80 wt % of bottle‐grade post‐consumer recycled poly(ethylene terephtalate) (r‐PET). Combinations of a commercial modifier with pentaerythritol were also successfully employed to cause simultaneous hyperbranching and controlled chain scission, thereby modifying the melt rheology of the material without excessively increasing the molecular weight, as highlighted by common technological melt viscosity measurements such as online torque and off‐line melt flow rate (MFR). Since the high melt fluidity of PET plays a critical role on its flame resistance, the combined effect of chain extenders and halogen‐free phosphorated additives on the fire resistance of the modified toughened blends was also investigated. Preliminary results indicate that the chemical reactions among polymer and additives must be taken into careful account to prevent unfavorable effects on the ultimate melt rheology and mechanical properties. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40881. 相似文献
18.
Chiara Bellocchi Angelica Carandina Beatrice Montinaro Elena Targetti Ludovico Furlan Gabriel Dias Rodrigues Eleonora Tobaldini Nicola Montano 《International journal of molecular sciences》2022,23(5)
The autonomic nervous system (ANS) and the immune system are deeply interrelated. The ANS regulates both innate and adaptive immunity through the sympathetic and parasympathetic branches, and an imbalance in this system can determine an altered inflammatory response as typically observed in chronic conditions such as systemic autoimmune diseases. Rheumatoid arthritis, systemic lupus erythematosus, and systemic sclerosis all show a dysfunction of the ANS that is mutually related to the increase in inflammation and cardiovascular risk. Moreover, an interaction between ANS and the gut microbiota has direct effects on inflammation homeostasis. Recently vagal stimulation techniques have emerged as an unprecedented possibility to reduce ANS dysfunction, especially in chronic diseases characterized by pain and a decreased quality of life as well as in chronic inflammation. 相似文献
19.
Beatrice Piola Maurizio Sabbatini Sarah Gino Marco Invernizzi Filippo Ren 《International journal of molecular sciences》2022,23(1)
In recent years, bioprinting has attracted much attention as a potential tool for generating complex 3D biological constructs capable of mimicking the native tissue microenvironment and promoting physiologically relevant cell–cell and cell–matrix interactions. The aim of the present study was to develop a crosslinked 3D printable hydrogel based on biocompatible natural polymers, gelatin and xanthan gum at different percentages to be used both as a scaffold for cell growth and as a wound dressing. The CellInk Inkredible 3D printer was used for the 3D printing of hydrogels, and a glutaraldehyde solution was tested for the crosslinking process. We were able to obtain two kinds of printable hydrogels with different porosity, swelling and degradation time. Subsequently, the printed hydrogels were characterized from the point of view of biocompatibility. Our results showed that gelatin/xanthan-gum bioprinted hydrogels were biocompatible materials, as they allowed both human keratinocyte and fibroblast in vitro growth for 14 days. These two bioprintable hydrogels could be also used as a helpful dressing material. 相似文献
20.
Giulia Sierri Roberta Dal Magro Barbara Vergani Biagio Eugenio Leone Beatrice Formicola Lorenzo Taiarol Stefano Fagioli Marcelo Kravicz Lucio Tremolizzo Laura Calabresi Francesca Re 《International journal of molecular sciences》2022,23(1)
The cerebral synthesis of cholesterol is mainly handled by astrocytes, which are also responsible for apoproteins’ synthesis and lipoproteins’ assembly required for the cholesterol transport in the brain parenchyma. In Alzheimer disease (AD), these processes are impaired, likely because of the astrogliosis, a process characterized by morphological and functional changes in astrocytes. Several ATP-binding cassette transporters expressed by brain cells are involved in the formation of nascent discoidal lipoproteins, but the effect of beta-amyloid (Aβ) assemblies on this process is not fully understood. In this study, we investigated how of Aβ1-42-induced astrogliosis affects the metabolism of cholesterol in vitro. We detected an impairment in the cholesterol efflux of reactive astrocytes attributable to reduced levels of ABCA1 transporters that could explain the decreased lipoproteins’ levels detected in AD patients. To approach this issue, we designed biomimetic HDLs and evaluated their performance as cholesterol acceptors. The results demonstrated the ability of apoA-I nanodiscs to cross the blood–brain barrier in vitro and to promote the cholesterol efflux from astrocytes, making them suitable as a potential supportive treatment for AD to compensate the depletion of cerebral HDLs. 相似文献