Development of a polymerase chain reaction assay for species identification of goose and mule duck in foie gras products

Polymerase chain reaction amplification of a conserved region of the -actin gene has been used for the specific identification of goose (Anser anser) and mule duck (Anas platyrhynchos×Cairina moschata) foie gras. Universal primers were used for the amplification of a DNA fragment containing three introns and four exons of the -actin gene in goose and mule duck. Sequence analysis of the amplified fragments was necessary for the design of forward species-specific primers in the goose and mule duck -actin genes. The use of species-specific forward primers, together with a reverse universal primer, produced amplicons of different length, allowing clear identification of goose and mule duck foie gras samples. Analysis of experimental mixtures demonstrated that 1% of duck can be easily detected in goose foie gras using the PCR method developed here. This genetic marker can be very useful for the accurate identification of these two species in foie gras products.     

Simultaneous lactic acid and xylitol production from vine trimming wastes

Hemicellulosic hydrolyzates from vineshoot trimmings obtained by dilute sulfuric acid hydrolysis were evaluated for xylitol production by Debaryomyces hansenii NRRL Y‐7426. Bioconversion was not efficient, however, since a mixture of products (mainly ethanol) was achieved. Taking into account that hexoses (such as glucose or mannose) can inhibit xylose metabolism by repression and inactivation of the xylose transport system or catabolic enzymes and that these hemicellulosic hydrolyzates are characterized by a high glucose concentration, a novel technology was developed, sequentially transforming glucose into lactic acid by Lactobacillus rhamnosus followed by fermentation of xylose into xylitol by Debaryomyces hansenii after L. rhamnosus removal by microfiltration. Optimal conditions were achieved using detoxified concentrated hemicellulosic hydrolyzates, after CaCO₃ addition in both stages of fermentation and using nitrogen purges after sampling in order to reduce the oxygen dissolved. Under these conditions 31.5 g lactic acid L^?1 (Q_LA = 1.312 g L^?1 h^?1 and Y_LA/S = 0.93 g g^?1) and 27.5 g xylitol L^?1 (Q_Xylitol = 0.458 g L^?1 h^?1 and Y_Xylitol/S = 0.53 g g^?1) were produced. Finally, lactic acid was selectively recovered using the resin Amberlite IRA 400 (0.0374 g of lactic acid g^?1 of dry resin), allowing a further recovery of xylitol by sequential stages of adsorption, concentration, ethanol precipitation, concentration and crystallization to obtain food‐grade xylitol according to a developed process. Copyright © 2007 Society of Chemical Industry     

Study of woven fabric shear behaviour

The paper presents the shear frame test with a new method of gripping the fabric sample to describe the shear behaviour of fabrics used in the garment industry under high shear strain conditions (maximum shear angles of 20°). This shear frame test was also used to compare between hysteresis curves given by the Kawabata evaluation system (KES) test, the bias extension test and the shear frame test for 10 woven fabrics. The linear viscoelastic theory was used to analyse the shear relaxation behaviour of the woven fabrics. In addition, in this study, the kernel function of the generalised Maxwell model has been used to describe the viscoelastic properties of the fabrics.     

Numerical and Experimental Study on Fluid Dynamic Features of Combined Gas and Electromagnetic Stirring in Ladle Furnace

Basic fluid dynamic features of combined electromagnetic stirring, EMS, and gas stirring (EMGAS) have been studied in the present work. A transient and turbulent multiphase numerical flow model was built. Simulations of a real size ladle furnace were conducted for 7 cases, operating with and without combined stirring and varying the argon gas inlet plug position. The results of these simulations are compared considering melt velocity, melt turbulence, melt/slag‐interface turbulence and dispersion of gas bubbles. An experimental water model was also built to simulate the effects of combined stirring. The water model was numerically simulated and visual comparison of the gas plume shape and flow pattern in the numerical and in the experimental model was also done for 3 flow situations. The results show that EMGAS has a strong flexibility regarding the flow velocity, gas plume, stirring energy, mixing time, slag layer, etc.     

BETaaS: A Platform for Development and Execution of Machine-to-Machine Applications in the Internet of Things

Wireless Personal Communications - The integration of everyday objects into the Internet represents the foundation of the forthcoming Internet of Things (IoT). Smart objects will be the building...     

Electron beam penetration of poly (methyl methacrylate)/colemanite composite irradiated at low earth orbit space radiation environment

Poly (methyl methacrylate) modification by colemanite (CMT) addition has ensured beta shielding improvement. This study has connected with space environmental utilization with Kibo Japanese Experiment Module on International Space Station at low earth orbit. Space environment experiment was performed by using Kibo Experiment Handrail Attachment Mechanism–ExHAM. The changes in beta transmission of the composite were examined (by Sr-90 radioisotope) before and after space environmental utilization and details on beta penetration capacity was examined to protect the equipment (used in aviation and aerospace industries) from space radiation. Beta attenuation variations have indicated composite densification with beta attenuation enhancement after CMT addition.