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921.
Ganglioside GM3, one of acidic components of membrane glycosphingolipids (GSL), has been known to change its content quantitatively during growth and differentiation of various cells in vitro. Detailed analysis of lipid portion of GM3 of rat renal glomerular SGE1 cells revealed that fatty acids with long carbon chains, especially that of C24:0 and C24:1 increased, while that of short C18:0 and C20:0 decreased after spontaneous dome formation. Since not only fatty acid composition of neutral GSL, sulfatide and phospholipid but also composition of long-chain bases (LCB) did not change, it was suggested that only C24 fatty acid of GM3 specifically increased in relation to dome formation. The spontaneous dome formation has been reported to be related with induction of cellular differentiation in many transporting epithelial cells. We thus assume that the change of fatty acid composition of GM3 is involved in cellular differentiation of SGE1 cells. 相似文献
922.
JY Jin DW Kim JW Lee CW Han WS Min CW Park CC Kim DJ Kim HK Kim HH Song 《Canadian Metallurgical Quarterly》1995,10(1):25-31
Calpains are intracellular cysteine proteases activated in a Ca(2+)-dependent manner. Previously, we found that the differentiation of K562 cells induced by 4 beta-phorbol 12-myristate 13-acetate treatment is accompanied by an increase in m-calpain levels and, at the same time, m-calpain becomes localized on the inside of plasma membranes, coated pits, and coated vesicles [Nakamura, M., Mori, M., Morishita, Y., Mori, S. & Kawashima, S. (1992) Exp. Cell Res. 200, 513-522]. We also reported that mu-calpain plays an essential role in morphological changes and membrane fusion of erythrocytes through the degradation of spectrin, a lining protein [Hayashi, M., Saito, Y. & Kawashima, S. (1992) Biochim. Biophys. Res. Commun. 182, 939-946]. Thus, calpains are implicated in endocytosis and/or exocytosis, processes stimulated by Ca2+ and involving intracellular membrane fusion. In this study, we report the biochemical characterization of calpains as components of purified coated vesicles from bovine brain. It was found by Western-blot analysis and chemical cross-linking of proteins that calpains are bound to the membranes of coated vesicles, and not to the coats. The binding of m-calpain to vesicles is Ca(2+)-dependent, while that of mu-calpain is less dependent on the presence of Ca2+. We also identified substrate proteins for calpains in coated vesicles. Upon activation of endogenous calpains, component proteins of coated vesicles such as the clathrin light chain, tubulins, and adaptins, but not the clathrin heavy chain, are highly sensitive to calpain digestion. In the case of exogenously added calpains, low concentrations degraded the same protein components. The degradation pattern differs slightly between added mu-calpain and m-calpain. These results strongly suggest that calpains are involved in the formation of coated vesicles and/or vesicle fusion to endosomes. 相似文献
923.
Photoactivated heterobifunctional cross-linking reagents which demonstrate the aggregation state of phospholipase A2 总被引:1,自引:0,他引:1
Two novel heterobifunctional cross-linking reagents, which can be used to attach photoactivatable nitroaryl azides to primary amino groups of proteins, have been synthesized. The two compounds, N-5-azido-2-nitrobenzoyloxy-succinimide and ethyl N-5-azido-2-nitrobenzoylaminoacet-imidate-HCl, as well as ethyl 4-azidobenzimidate-HCl have been attached to lysine residues of cobra venom phospholipase A2 without a loss in enzymatic activity. Subsequent illumination of the modified forms of the enzyme at appropriate wavelengths under conditions in which the native enzyme exists in an aggregated state led to the formation of covalently linked dimers and large aggregates which could be separated by electrophoresis on polyacrylamide gels in the presence of sodium dodecyl sulfate. 相似文献
924.
JJ Bookstein KM Moser ME Kalafer CB Higgins GB Davis WS James 《Canadian Metallurgical Quarterly》1977,72(5):658-661
A case is presented which demonstrates the value of bronchial arteriographic studies in localizing and treating hemoptysis. The patient was bleeding from an old post-tuberculous bulla and for several reasons was not a candidate for surgery. Bronchial arteriographic studies demonstrated both hypervascularity in the region of the cavity and also a mycotic aneurysm of a bronchial artery. After therapeutic embolization with gelatin (Gelfoam), the hypervascularity and aneurysm were no longer opacified. Active bleeding abruptly ceased and, except for a single mild recurrence, has not recurred during the three months since therapeutic embolization. The major theoretic risk of bronchial arteriographic study and therapeutic embolization is spinal injury. It is believed that permanent injury can almost be avoided, providing proper technique is used. 相似文献
925.
A novel technique is described in which the effect of the beta-adrenoceptor antagonists timolol and carteolol, and the vasodilators sodium nitroprusside (SNP) and verapamil on intraocular pressure (IOP) and the distribution of ocular flow in the bovine arterially perfused eye is investigated using radiolabelled microspheres. At maximum IOP-reducing dose timolol was found to significantly reduce perfusion in the choroid and, at higher dose, it was found to significantly reduce perfusion in the iris. By contrast, a maximal IOP-reducing dose of carteolol markedly reduced perfusion in the iris, ciliary body and choroid. Vasoconstriction induced by carteolol was not inhibited by the alpha-antagonist phentolamine. Against a background of vascular tone induced by noradrenaline, SNP and verapamil were found to significantly increase perfusion in the iris, ciliary body and choroid. The effects of these drugs upon the vasculature of the bovine perfused eye are varied and complex and may not bear a direct relationship to their ocular hypotensive effect. 相似文献
926.
RP Karlsberg I Eisenstein WS Aronow J Edelstein PA Chandraratna 《Canadian Metallurgical Quarterly》1980,66(1):18-26
2 cases of right coronary artery aneurysm visualized with M-mode and cross-sectional echocardiography are described. Echocardiographic visualization of right coronary artery aneurysms is possible when substantial dilation is located proximally. 相似文献
927.
928.
929.
ME Mitchell AC Waltman CA Athanasoulis WS Kerr SP Dretler 《Canadian Metallurgical Quarterly》1976,115(6):692-695
In the 4 cases reported herein angiographic techniques with selective bilateral hypogastric embolization were used to control severe prostatic hemorrhage. The technique is offered as an alternative, non-operative procedure in the management of bleeding when other modes of therapy contain significantly higher potentials for morbidity and mortality. 相似文献