A simple spectrophotometric method for estimating total glucosinolates in mustard de-oiled cake

Glucosinolates are anti-nutritional factors present abundantly in the seed meal fraction of oilseed Brassica species. They are found in varying levels among different genotypes. Those genotypes containing less than 30 µmol/g are considered low/zero glucosinolate type and are preferred for edible purposes due to low pungency. Twenty two different genotypes were taken for the analysis of glucosinolates by spectrophotometry. A regression model was obtained using Ordinary Least Square technique which predicted a formula. Total glucosinolates (µmol/g) = 1.40 + 118.86 × A₄₂₅, where A₄₂₅ is the absorbance at 425 nm. The total glucosinolate content obtained by the prediction formula when compared with HPLC data showed a correlation coefficient of 0.942. This high correlation between the two data sets validated the developed methodology. This method also simplifies the estimation of total glucosinolates by excluding the use of HPLC or other sophisticated instruments.     

Effects of soil and crop management practices on yields,income and nutrients losses from upland farming systems in the Middle Mountains region of Nepal

On-farm runoff plots were established during 2004 and monitored for 4 years in the Pokhare Khola watershed (Nepal) in a completely randomized design with four replications of each three treatments: traditional Farmer Practice (FP) (Zea mays–Eleusine coracana), Reduced Tillage (RT; Z. mays–Vigna ungeuculata), and Commercial Vegetable with double dose of farm yard manure (CV; Z. mays–Capsicum species) to evaluate treatment effects on soil nutrient losses, nutrient balances and crop income on Bari land (rainfed terraces). Nutrient removal due to crop harvest was found to be significantly higher than nutrient loss through soil erosion, and CV treatment exhibited a significantly higher N uptake (123 kg ha⁻¹ year⁻¹) through crop harvest than other treatments. Moreover, the CV treatment produced significantly higher income per unit area of Bari land than the other treatments. Soil organic carbon and major nutrients losses (NPK) through soil erosion were minimal [25.5 kg ha⁻¹ year⁻¹ soil organic carbon (SOC) and 5.6:0.02:0.12 kg ha⁻¹ year⁻¹ nitrogen (N), phosphorus (P), potassium (K), respectively]. Result showed that no nutrients were lost through leaching. Nutrient losses due to soil erosion and runoff were lower than previously reported in the Middle Mountain region, indicating a need to re-evaluate the soil erosion and nutrient loss problems in this region. Interventions such as reduced tillage and double dose of FYM with vegetable production were found to be effective in maintaining soil fertility and increasing farm income compared to the traditional maize-millet production system. The nutrient balance calculations suggest that integrated nutrient management techniques such as residue incorporation and application of FYM with a minimum application of chemical fertilizer are potentially sustainable production approaches for the Mid-hills of Nepal.     

A review on solid oxide derived from waste shells as catalyst for biodiesel production

The waste eggs and mollusk shells are found to be the richest sources of calcium carbonate and have been utilized for various purposes after proper treatments. When calcined at a proper temperature calcium carbonate converts into CaO, which is a metal oxide. Researchers have found that the CaO prepared from the waste shells can be used as catalyst in biodiesel production process. Utilization of waste shells as a source of CaO not only gives an opportunity to use it as catalyst but also adds value to the waste generated. In this paper a brief discussion with recent development on biodiesel production using waste shell derived solid oxide as catalyst is presented.     

Software reliability growth supermodels

The aim of this paper is to present a method for obtaining a more accurate prediction for software reliability growth models (SRGMs). It is our belief that if we try to use a more general approach implying the building of a supermodel as a weighted sum of several SRGMs, it may be possible to obtain more accurate results in prediction. The weight factors will depend on the values of the prequential likelihood functions as calculated for each SRGM, the values varying each time a new error is observed. The basic models chosen are the Jelinski-Moranda, Goel-Okumoto, Duane, Littlewood-Verrall and Keiller-Littlewood models. Finally, we shall compare the SRGMs with the supermodels by using the median estimate and deciding if there are any benefits or constraints in applying this technique.     

Changes in macroelements,trace elements,heavy metal concentrations and chemical composition in milk of Croatian spotted goats during different lactation stages

The research was conducted on days 60, 90, 120 and 150 during the lactation with the aim to determine the effect of lactation stage on the concentration of crucial elements and chemical composition in Croatian spotted goats' milk. Milk yield significantly decreased (from 1.15 to 0.76 kg/day), while the concentration of Ca, Mg, P, Zn and Mo in the milk of the goats significantly increased during lactation. The concentrations of Cr, Cd and Pb in milk were very low, with no significant deviations during lactation. The quality of milk from Croatian spotted goats is adequate compared with other breeds in the surrounding area of Croatia.     

Immunochromatographic dipstick assay format using gold nanoparticles labeled protein-hapten conjugate for the detection of atrazine

The present study describes a lateral-flow-based dipstick immunoassay format using a novel hapten-protein-gold conjugate for the rapid screening of atrazine in water samples. The immunoassay is based on the competitive inhibition, in which a newly developed hapten-protein-gold conjugate competes with the free antigen present in the sample, for the limited antibody binding sites available at test zone on dipstick membrane, housed in a plastic cartridge. The tracer used as the detection reagent was prepared by first conjugating hapten (a derivative of atrazine) molecules to a carrier protein (bovine serum albumin) via its surface lysine residues and then linking colloidal gold nanoparticles to the hapten-protein conjugate via cysteine residues of the carrier protein. The developed conjugate showed a high level of stability as it did not show any significant loss of activity even after 8 weeks of storage at ambient conditions. The color developed due to conjugate, based on competitive inhibition approach, is correlated with the concentration of atrazine sample. The sensitivity of the developed dipstick was enhanced by gold nanoparticles, as an amplification tag, presenting detection limit of atrazine in standard water samples down to 1.0 ppb level. The kit could serve as a rapid screening methodology for visual screening of atrazine contamination of water samples within 5 min of analysis time, and, when coupled with a portable colorimeter, as an inexpensive semi-quantitative assay. The method reported can be useful for screening a large number of pesticides samples in a very short time in the field.     

DyProSD: a dynamic protocol specific defense for high-rate DDoS flooding attacks

Microsystem Technologies - High-rate distributed denial of service (HDDoS) flooding attacks pose as a major threat to the Internet. Most present solutions based on machine learning approach are...